A bioreducible supramolecular nanoparticle gene delivery system based on cyclodextrin-conjugated polyaspartamide and adamantyl-terminated polyethylenimine

2018 ◽  
Vol 6 (5) ◽  
pp. 797-808 ◽  
Author(s):  
Yunti Zhang ◽  
Qimin Jiang ◽  
Bo Bi ◽  
Luming Xu ◽  
Jia Liu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Cellular Internalization ◽  
Gene Delivery System ◽  
Supramolecular Nanoparticles

Reduction degradable Pasp-SS-CD/Ad4-PEI/pDNA supramolecular nanoparticles via host–guest interaction exhibited improved cellular internalization and higher gene transfection efficiency with lower cytotoxicity.

The Combination of Drug or Gene Delivery System Responding to Cellular Signals (D-RECS) and Sonoporation System for Effective and Safe Gene Delivery

2009 ◽  
Vol 1237 ◽  
Author(s):  
Akira Tsuchiya ◽  
Takeshi Mori ◽  
Yuki Naritomi ◽  
Jeong-Hun Kang ◽  
Daisuke Asai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Regulation ◽  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Regulation System ◽  
Gene Delivery System

AbstractWe have developed new gene expression-regulating polymer that can activate transgene expression in response to target intracellular signals. Here, we tried applying sonoporation system to this gene regulation system to enhance the gene expression efficacy. Sonoporation is the method for effective gene transfection in vitro and in vivo. Therefore, the method might enhance the transfection efficiency in our polymer and realize an efficient and safe gene delivery system. Results suggested that the combination of our polymer and sonoporation could improve the gene expression compared to the system using only our polymer that transfers genes into cells via endocytosis. It also kept the ability of the gene regulation responding to cellular signals.

A Review on Gene Delivery System of Layered Double Hydroxide

2013 ◽  
Vol 320 ◽  
pp. 533-539
Author(s):  
Li E Dong ◽  
Guo Jing Gou ◽  
Jie Huang ◽  
Yue Sun ◽  
Bing Xue
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Release Mechanism ◽  
Gene Delivery System ◽  
Dna Fragment ◽  
Double Hydroxide ◽  
Double Hydroxides ◽  
Dna Complex

This paper aims to provide a concise review on the study of a gene delivery system with layered double hydroxides (LDH) as carrier. The issues, such as preparation and cytobiology effect of LDH-DNA hybrids, and the release mechanism of DNA from LDH-DNA complex, were discussed in detail. For achieving a ideal cell transfection efficiency, it would be prospective to improve both the intercalation effect of giant DNA, such as plasmid DNA and long chain DNA fragments, and the gene transfection technique of small DNA fragment.

Receptor-Mediated Gene Delivery Using Chitosan Derivatives In Vitro and In Vivo

2007 ◽  
Vol 342-343 ◽  
pp. 449-452 ◽  
Author(s):  
Tae Hee Kim ◽  
Hua Jin ◽  
Hyun Woo Kim ◽  
Myung Haing Cho ◽  
Jae Woon Nah ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Viral Gene ◽  
Gene Delivery System ◽  
Targeted Gene Delivery ◽  
Cell Specificity ◽  
Selective Delivery

The key strategy for the advancement of gene therapy is the development of an efficient targeted gene delivery system into cells. The targeted gene delivery system is especially important in non-viral gene transfer which shows the relatively low transfection efficiency. It also opens the possibility of selective delivery of therapeutic plasmids to specific tissues. Chitosan has been considered to be a good candidate for gene delivery system, since it is already known as a biocompatible, biodegradable, and low toxic material with high cationic potential. However, low specificity and low transfection efficiency of chitosan need to be overcome prior to clinical trial. In this study, we focused on the chemical modification of chitosan for enhancement of cell specificity and transfection efficiency. Also, the potential of clinical application was investigated.

Zinc ion coordination significantly improved the transfection efficiency of low molecular weight polyethylenimine

2019 ◽  
Vol 7 (4) ◽  
pp. 1716-1728 ◽  
Author(s):  
Huapan Fang ◽  
Lin Lin ◽  
Jie Chen ◽  
Jiayan Wu ◽  
Huayu Tian ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Zinc Ion ◽  
Low Molecular Weight ◽  
Gene Delivery System ◽  
System P

A zinc ion coordination-contained polycationic gene delivery system.

A Poly(β-Amino Ester) of Spermine and Poly(ethylene Glycol) Diacrylate as a Gene Carrier

2007 ◽  
Vol 342-343 ◽  
pp. 425-428 ◽  
Author(s):  
Dhananjay Jere ◽  
Tae Hee Kim ◽  
Rohidas B. Arote ◽  
Hu Lin Jiang ◽  
Myung Haing Cho ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ethylene Glycol ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Water Soluble ◽  
Gene Carrier ◽  
Gene Delivery System ◽  
Poly Ethylene Glycol ◽  
Wide Range ◽  
Poly Ethylene

Vectors are vital aspect of gene delivery system which decides the success of gene therapy. Efficient transfection with minimum or no toxicity, are two principal aims of any gene delivery system. In this our study, we rationally developed biodegradable water soluble poly(ßamino ester) (PAE) based on spermine (SPR) and poly (ethylene glycol) (PEG), by Michael-type addition reaction and further studied for its potential as a gene carrier. Confirmation of synthesized PAE was done by proton NMR spectroscopy. In gel retardation assay, the PAEs have shown good DNA binding ability over wide range of polyplexes. The addition of PEG over SPR resulted in a novel PAE with higher degree of safety and transfection efficiency as compared with polyethylenimine 25K (PEI) when studied in 293T human kidney carcinoma cells.

Dual-functionalized calcium carbonate based gene delivery system for efficient gene delivery

RSC Advances ◽  
2014 ◽  
Vol 4 (73) ◽  
pp. 38623-38629 ◽  
Author(s):  
Chao-Qun Wang ◽  
Meng-Qing Gong ◽  
Jin-Long Wu ◽  
Ren-Xi Zhuo ◽  
Si-Xue Cheng
Keyword(s):  
Calcium Carbonate ◽  
Gene Delivery ◽  
Delivery System ◽  
Gene Transfection ◽  
Cell Penetrating Peptide ◽  
Gene Delivery System ◽  
Dna Nanoparticles ◽  
Efficient Gene ◽  
Cell Penetrating ◽  
A Cell

Dual-functionalized KALA/PS/CaCO3/DNA nanoparticles containing a cell penetrating peptide (KALA) and protamine sulfate (PS) could effectively mediate gene transfection at a low DNA concentration.

CAGW and TAT‐NLS peptides functionalized multitargeting gene delivery system with high transfection efficiency

2019 ◽  
Vol 30 (10) ◽  
pp. 2567-2576
Author(s):  
Xinghong Duo ◽  
Lingchuang Bai ◽  
Jun Wang ◽  
Hao Ji ◽  
Jintang Guo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Gene Delivery System ◽  
High Transfection Efficiency
Sign in / Sign up

Export Citation Format

Share Document