Devising new lipid-coated calcium phosphate/carbonate hybrid nanoparticles for controlled release in endosomes for efficient gene delivery

2017 ◽  
Vol 5 (34) ◽  
pp. 7194-7203 ◽  
Author(s):  
Yilun Wu ◽  
Wenyi Gu ◽  
Jie Tang ◽  
Zhi Ping Xu
Keyword(s):  
Controlled Release ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Cancer Cells ◽  
Hybrid Nanoparticles ◽  
Efficient Gene ◽  
Endosomal Ph ◽  
Ph Range ◽  
Phosphate Carbonate

New hybrid nanoparticles can efficiently deliver dsDNA/siRNA to cancer cells, with the gene release precisely controlled in the endosomal pH range.

High-Effective Gene Delivery Based on Cyclodextrins Multivalent Assembly in Target Cancer Cells

2022 ◽  
Author(s):  
Yao-Hua Liu ◽  
Yu Liu
Keyword(s):  
Gene Therapy ◽  
Controlled Release ◽  
Gene Delivery ◽  
Nucleic Acids ◽  
Cancer Cells ◽  
Chemical Biology ◽  
Supramolecular Assembly ◽  
Target Cancer

Nucleic acids condensation and controlled release remain significant challenges of gene therapy in chemical biology and nanotechnology fields. In this work, we have reported a polysaccharide supramolecular assembly constructed by...

scholarly journals Preparation of optimized lipid-coated calcium phosphate nanoparticles for enhanced in vitro gene delivery to breast cancer cells

2015 ◽  
Vol 3 (33) ◽  
pp. 6805-6812 ◽  
Author(s):  
Jie Tang ◽  
Li Li ◽  
Christopher B. Howard ◽  
Stephen M. Mahler ◽  
Leaf Huang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Calcium Phosphate ◽  
Cell Growth ◽  
Gene Delivery ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cancer Cell Growth ◽  
Calcium Phosphate Nanoparticles ◽  
Transfection Agent

The optimized lipid coated calcium phosphate nanoparticles more efficiently deliver functional siRNA and inhibit the cancer cell growth, in comparison with the commercial transfection agent OligofactamineTM.

Efficient Gene Delivery with Serum into Human Cancer Cells Using Targeted Anionic Liposomes

2004 ◽  
Vol 12 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Mohsen M. Mady ◽  
M.M. Ghannam ◽  
W.A. Khalil ◽  
R. Repp ◽  
M. Markus ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cancer Cells ◽  
Human Cancer ◽  
Human Cancer Cells ◽  
Efficient Gene ◽  
Anionic Liposomes

scholarly journals A Valid Bisphosphonate Modified Calcium Phosphate-Based Gene Delivery System: Increased Stability and Enhanced Transfection Efficiency In Vitro and In Vivo

Pharmaceutics ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 468 ◽  
Author(s):  
Zhao ◽  
Li ◽  
Chen ◽  
Hu
Keyword(s):  
Calcium Phosphate ◽  
Gene Delivery ◽  
Tissue Specificity ◽  
Transfection Efficiency ◽  
Hybrid Nanoparticles ◽  
In Vivo Studies ◽  
Adsorptive Capacity ◽  
Low Cytotoxicity

Calcium phosphate (CaP) nanoparticles, as a promising vehicle for gene delivery, have been widely used owing to their biocompatibility, biodegradability and adsorptive capacity for nucleic acids. Unfortunately, their utility in vivo has been profoundly restricted due to numerous technical barriers such as the lack of tissue specificity and limited transfection efficiency, as well as uncontrollable aggregation over time. To address these issues, an effective conjugate folate-polyethylene glycol-pamidronate (shortened as FA-PEG-Pam) was designed and coated on the surface of CaP/NLS/pDNA (CaP/NDs), forming a versatile gene carrier FA-PEG-Pam/CaP/NDs. Inclusion of FA-PEG-Pam significantly reduced the size of CaP nanoparticles, thus inhibiting the aggregation of CaP nanoparticles. FA-PEG-Pam/CaP/NDs showed better cellular uptake than mPEG-Pam/CaP/NDs, which could be attributed to the high-affinity interactions between FA and highly expressed FR. Meanwhile, FA-PEG-Pam/CaP/NDs had low cytotoxicity and desired effect on inducing apoptosis (71.1%). Furthermore, FA-PEG-Pam/CaP/NDs showed admirable transfection efficiency (63.5%) due to the presence of NLS peptides. What’s more, in vivo studies revealed that the hybrid nanoparticles had supreme antitumor activity (IR% = 58.7%) among the whole preparations. Altogether, FA-PEG-Pam/CaP/NDs was expected to be a hopeful strategy for gene delivery.

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