A graphene–polyurethane composite hydrogel as a potential bioink for 3D bioprinting and differentiation of neural stem cells

2017 ◽  
Vol 5 (44) ◽  
pp. 8854-8864 ◽  
Author(s):  
Chao-Ting Huang ◽  
Lok Kumar Shrestha ◽  
Katsuhiko Ariga ◽  
Shan-hui Hsu
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Composite Hydrogel ◽  
3D Bioprinting ◽  
Tissue Constructs ◽  
Polyurethane Composite ◽  
Associated Proteins

The composite hydrogel ink containing a small amount of graphene (25 ppm) was printed with neural stem cells (NSCs) into 3D cell-laden tissue constructs, expressing neural-associated proteins after culture for only seven days without induction.

scholarly journals 3D bioprinting of co-cultured osteogenic spheroids for bone tissue fabrication

2020 ◽  
Author(s):  
Dong Nyoung Heo ◽  
Bugra Ayan ◽  
Madhuri Dey ◽  
Dishary Banerjee ◽  
Hwabok Wee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Building Blocks ◽  
Specific Gene ◽  
3D Bioprinting ◽  
Cell Seeding ◽  
Native Bone ◽  
Tissue Constructs

AbstractConventional top-down approaches in tissue engineering involving cell seeding on scaffolds have been widely used in bone engineering applications. However, scaffold-based bone tissue constructs have had limited clinical translation due to constrains in supporting scaffolds, minimal flexibility in tuning scaffold degradation, and low achievable cell seeding density as compared with native bone tissue. Here, we demonstrate a pragmatic and scalable bottom-up method, inspired from embryonic developmental biology, to build three-dimensional (3D) scaffold-free constructs using spheroids as building blocks. Human umbilical vein endothelial cells (HUVECs) were introduced to human mesenchymal stem cells (hMSCs) (hMSC/HUVEC) and spheroids were fabricated by an aggregate culture system. Bone tissue was generated by induction of osteogenic differentiation in hMSC/HUVEC spheroids for 10 days, with enhanced osteogenic differentiation and cell viability in the core of the spheroids compared to hMSC-only spheroids. Aspiration-assisted bioprinting (AAB) is a new bioprinting technique which allows precise positioning of spheroids (11% with respect to the spheroid diameter) by employing aspiration to lift individual spheroids and bioprint them onto a hydrogel. AAB facilitated bioprinting of scaffold-free bone tissue constructs using the pre-differentiated hMSC/HUVEC spheroids. These constructs demonstrated negligible changes in their shape for two days after bioprinting owing to the reduced proliferative potential of differentiated stem cells. Bioprinted bone tissues showed interconnectivity with actin-filament formation and high expression of osteogenic and endothelial-specific gene factors. This study thus presents a viable approach for 3D bioprinting of complex-shaped geometries using spheroids as building blocks, which can be used for various applications including but not limited to, tissue engineering, organ-on-a-chip and microfluidic devices, drug screening and, disease modeling.

Sign in / Sign up

Export Citation Format

Share Document