In vivo self-assembly induced retention of gold nanoparticles for enhanced photothermal tumor treatment

2017 ◽  
Vol 5 (30) ◽  
pp. 5931-5936 ◽  
Author(s):  
Xuefeng Hu ◽  
Peipei Yang ◽  
Jianping He ◽  
Ruijie Liang ◽  
Dechao Niu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Self Assembly ◽  
Retention Performance ◽  
Tumor Treatment ◽  
Simple Route ◽  
Photothermal Treatment ◽  
Spherical Gold Nanoparticles

A simple route to fabricate peptide modified spherical gold nanoparticles with enhanced retention performance in tumor sites for improved photothermal treatment.

Bio-distribution of Gold Nanoparticles in Tumor Mass and Different Organs in Implanted Mice with Mammary Adenocarcinoma AM3 (in vivo study)

2019 ◽  
Vol 43 (2) ◽  
pp. 17-22
Author(s):  
Sumaiah I Hussein
Keyword(s):  
Gold Nanoparticles ◽  
Intraperitoneal Administration ◽  
Kidney Tissue ◽  
The Other ◽  
Mammary Adenocarcinoma ◽  
Tumor Treatment ◽  
Medical Field ◽  
Tumor Mass ◽  
Kidney Liver

Nanoparticles has many properties, especially in treatment of medical field, but the controversy continues about their cytotoxicity. Hence, this research was conducted to estimate the concentration of gold nanoparticles (GNPs) in tumor and other organs such as kidney, liver, and spleen after injection GNPs by two routes, intratumor and intraperitoneal to compare between two methods in mice implanted with mammary adenocarcinoma for 28 days. Atomic Absorption Spectroscopy was used to measure the GNPs concentrations. The results revealed that the GNPs concentrations were significantly (P≤0.05) increased (3.75±1.75, 2.42 ±0.31 ppm) in kidney tissue after intratumor and intraperitoneal administration, respectively, when compared to the other organs (liver and spleen), followed by tumor mass (2.66±0.01, 1.09 ±0.06 ppm) in tissue. While the concentrations of GNPs in spleen and liver were (1.40 ±0.33, 0.726 ±0.01) and (0.602 ±0.03, 0.517 ±0.02) after intratumor and intraperitoneal administration respectively. Also, the experiment showed that the injection by intratumor was more efficient than intraperitonial method for tumor treatment, so, the nanoparticles were cleared by responsible lymphoid organs of body.

scholarly journals Single-step assembly of gold nanoparticles into plasmonic colloidosomes at the interface of oleic acid nanodroplets

2021 ◽  
Vol 3 (1) ◽  
pp. 198-205
Author(s):  
José M. López-de-Luzuriaga ◽  
Miguel Monge ◽  
Javier Quintana ◽  
María Rodríguez-Castillo
Keyword(s):  
Gold Nanoparticles ◽  
Oleic Acid ◽  
Self Assembly ◽  
Single Step ◽  
The Self ◽  
Au Nps ◽  
Spherical Gold Nanoparticles

Plasmonic gold colloidosomes (Au CSs) of sub-200 nm size are formed by the self-assembly of spherical gold nanoparticles (Au NPs) of ca. 4 nm size at the interface of oleic acid (OA) nanodroplets formed in n-hexane.

scholarly journals In Vivo Study of Spherical Gold Nanoparticles: Inflammatory Effects and Distribution in Mice

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e58208 ◽  
Author(s):  
Hui Chen ◽  
Alisha Dorrigan ◽  
Sonia Saad ◽  
Dominic J. Hare ◽  
Michael B. Cortie ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
In Vivo Study ◽  
Spherical Gold Nanoparticles

scholarly journals Ultrathin Gold Nanowires to Enhance Radiation Therapy

2020 ◽  
Author(s):  
Lin Bai ◽  
Fangchao Jiang ◽  
Renjie Wang ◽  
Chaebin Lee ◽  
Hui Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gold Nanoparticles ◽  
Radiation Therapy ◽  
Xenograft Model ◽  
Gold Nanowires ◽  
Gold Nanospheres ◽  
Radical Production ◽  
Spherical Gold Nanoparticles

Abstract Background: Radiation therapy is a main treatment option for cancer. Due to normal tissue toxicity, radiosensitizers are commonly used to enhance RT. In particular, heavy metal or high-Z materials, such as gold nanoparticles, have been investigated as radiosensitizers. So far, however, the related studies have been focused on spherical gold nanoparticles. In this study, we assessed the potential of ultra-thin gold nanowires as a radiosensitizer, which is the first time. Methods: Gold nanowires were synthesized by the reduction of HAuCl4 in hexane. The as-synthesized gold nanowires were then coated with a layer of PEGylated phospholipid to be rendered soluble in water. Spherical gold nanoparticles coated with the same phospholipid were also synthesized as a comparison. Gold nanowires and gold nanospheres were first tested in solutions for their ability to enhance radical production under irradiation. They were then incubated with 4T1 cells to assess whether they could elevate cell oxidative stress under irradiation. Lastly, gold nanowires and gold nanoparticles were intratumorally injected into a 4T1 xenograft model, followed by irradiation applied to tumors (3 Gy/per day for three days). Tumor growth was monitored and compared. Results: Our studies showed that gold nanowires are superior to gold nanospheres in enhancing radical production under X-ray radiation. In vitro analysis found that the presence of gold nanowires caused elevated lipid peroxidation and intracellular oxidative stress under radiation. When tested in vivo, gold nanowires plus irradiation led to better tumor suppression than gold nanospheres plus radiation. Moreover, gold nanowires were found to be gradually reduced to shorter nanowires by glutathione, which may benefit fractionated radiation. Conclusion: Our studies suggest that gold nanowires are a promising type of radiosensitizer that can be safely injected into tumors to enhance radiotherapy. While the current study was conducted in a breast cancer model, the approach can be extended to the treatment of other cancer types.

scholarly journals Preparation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside and their evaluation in vivo

2019 ◽  
Author(s):  
Gennady L Burygin ◽  
Polina I Abronina ◽  
Nikita M Podvalnyy ◽  
Sergey A Staroverov ◽  
Leonid O Kononov ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Wall ◽  
Synthetic Polymers ◽  
Enzyme Linked Immunosorbent Assay ◽  
Weak Response ◽  
E Coli ◽  
Serological Identification ◽  
Spherical Gold Nanoparticles ◽  
Preparation And Evaluation

Some bacterial glycans are specific markers for detection and serological identification of microorganisms and are widely used as antigenic components of vaccines. The use of gold nanoparticles as carriers for glyco-epitopes is becoming an important alternative to the traditional conjugation with proteins and synthetic polymers. In this study, we aimed at preparation and evaluation in vivo of glyco-gold nanoparticles (glyco-GNPs) bearing the terminal branched hexaarabinofuranoside fragment (Ara6) of arabinan domains of lipoarabinomannan and arabinogalactan, which are principal polysaccharides of the cell wall of Mycobacterium tuberculosis, the causative agent of tuberculosis. Specifically, we were interested in checking if the antibodies generated against Ara6-GNPs would recognize the natural saccharides on the cell surface of different mycobacterial strains. Two synthetic Ara6 glycosides with amino-functionalized spacer aglycons differing in length and hydrophilicity were directly conjugated with spherical gold nanoparticles (d = 15 nm) to give two sets of glyco-GNPs which were used for immunization of rabbits. Dot assay revealed cross-reactions for the two obtained antisera with the hexaarabinofuranoside with 2-aminoethyl aglycon used for the preparation of glyco-GNPs. Both antisera contained high titers of antibodies specific for Mycobacteria as shown by enzyme-linked immunosorbent assay using M. bovis and M. smegmatis cells as antigens while gave a weak response with M. phlei cells and no interaction with E. coli cells. The results obtained suggest that glyco-GNPs are promising agents for generation of anti-mycobacterial antibodies.

scholarly journals Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

2020 ◽  
Vol 11 ◽  
pp. 480-493 ◽  
Author(s):  
Gennady L Burygin ◽  
Polina I Abronina ◽  
Nikita M Podvalnyy ◽  
Sergey A Staroverov ◽  
Leonid O Kononov ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Wall ◽  
Synthetic Polymers ◽  
Enzyme Linked Immunosorbent Assay ◽  
In Vivo Evaluation ◽  
Weak Response ◽  
E Coli ◽  
Serological Identification ◽  
Spherical Gold Nanoparticles

A number of bacterial glycans are specific markers for the detection and the serological identification of microorganisms and are also widely used as antigenic components of vaccines. The use of gold nanoparticles as carriers for glyco-epitopes is becoming an important alternative to the traditional conjugation with proteins and synthetic polymers. In this study, we aimed to prepare and evaluate in vivo glyco-gold nanoparticles (glyco-GNPs) bearing the terminal-branched hexaarabinofuranoside fragment (Ara6) of arabinan domains of lipoarabinomannan and arabinogalactan, which are principal polysaccharides of the cell wall of Mycobacterium tuberculosis, the causative agent of tuberculosis. In particular, we were interested whether the antibodies generated against Ara6-GNPs would recognize the natural saccharides on the cell surface of different mycobacterial strains. Two synthetic Ara6 glycosides with amino-functionalized spacer aglycons differing in length and hydrophilicity were directly conjugated with spherical gold nanoparticles (d = 15 nm) to give two sets of glyco-GNPs, which were used for the immunization of rabbits. Dot assays revealed cross-reactions between the two obtained antisera with the hexaarabinofuranoside and the 2-aminoethyl aglycon used for the preparation of glyco-GNPs. Both antisera contained high titers of antibodies specific for Mycobacteria as shown by enzyme-linked immunosorbent assay using M. bovis and M. smegmatis cells as antigens while there was only a weak response to M. phlei cells and no interaction with E. coli cells. The results obtained suggest that glyco-GNPs are promising agents for the generation of anti-mycobacterial antibodies.

Polymer anchored gold nanoparticles: Synthesis, characterization and antimicrobial activities

2020 ◽  
Vol 10 ◽  
Author(s):  
Pranesh Chowdhury ◽  
Bishnupada Roy ◽  
Suprabhat Mukherjee ◽  
Niladri Mukherjee ◽  
Nikhilesh Joardar ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Near Infrared ◽  
Wistar Rat ◽  
Antimicrobial Activities ◽  
X Rays ◽  
Photothermal Treatment ◽  
Surface Enhanced Raman ◽  
Polymer Anchored

Abstract : Gold nanoparticles (GNPs) have diverse optical and photo-thermal properties. It is used for diagnostic imaging because of their ability to absorb near infrared (NIR) and X-rays, and their surface enhanced Raman scattering properties. Recently they are used for drug delivery and more particularly photothermal treatment. Their ability to absorb NIR energy and convert it to heat by a Surface Plasmon Resonance mechanism has made these materials promising for the treatment of tumors. GNPs having diameter higher than 1.4–1.5 nm is found nontoxic to the environment. In the present work, polymer anchored GNPs are synthesized by reducing AuIII to Au0 in presence of either chitosan or polyvinyl alcohol, which act as in situ reducers cum stabilizers. Microscopic techniques (TEM, SEM and DLS) are used to analyse the size, surface morphology and size distribution respectively. The unique mucoadhesive properties of chitosan particularly make the system promising with respect to antimicrobial (anti-bacteria as well as anti-fungus) activities. An attempt has been made to understand the mechanistic path involved in antimicrobial activities. Antimicrobial potential of chitosan anchored gold nanoparticles (GNPc) are noticed even at very low dose. The results of bio-chemical analysis (MDA, NBT, DNA fragmentation and over-expression of heat shock protein) clearly explain antimicrobial activities. Additionally, gold chitosan systems interact with microbial DNA and inhibit the action of DNA repair enzyme. Interestingly, in vitro (rat peritoneal MФ) or in vivo (Wistar rat) analysis exhibits negligible cytotoxicy. Thus the synthesized material (particularly GNPc) is promising as an effective nano therapeutic agent.

scholarly journals Ultrathin gold nanowires to enhance radiation therapy

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Lin Bai ◽  
Fangchao Jiang ◽  
Renjie Wang ◽  
Chaebin Lee ◽  
Hui Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gold Nanoparticles ◽  
Radiation Therapy ◽  
Xenograft Model ◽  
Gold Nanowires ◽  
Gold Nanospheres ◽  
Radical Production ◽  
Spherical Gold Nanoparticles

Abstract Background Radiation therapy is a main treatment option for cancer. Due to normal tissue toxicity, radiosensitizers are commonly used to enhance RT. In particular, heavy metal or high-Z materials, such as gold nanoparticles, have been investigated as radiosensitizers. So far, however, the related studies have been focused on spherical gold nanoparticles. In this study, we assessed the potential of ultra-thin gold nanowires as a radiosensitizer, which is the first time. Methods Gold nanowires were synthesized by the reduction of HAuCl4 in hexane. The as-synthesized gold nanowires were then coated with a layer of PEGylated phospholipid to be rendered soluble in water. Spherical gold nanoparticles coated with the same phospholipid were also synthesized as a comparison. Gold nanowires and gold nanospheres were first tested in solutions for their ability to enhance radical production under irradiation. They were then incubated with 4T1 cells to assess whether they could elevate cell oxidative stress under irradiation. Lastly, gold nanowires and gold nanoparticles were intratumorally injected into a 4T1 xenograft model, followed by irradiation applied to tumors (3 Gy/per day for three days). Tumor growth was monitored and compared. Results Our studies showed that gold nanowires are superior to gold nanospheres in enhancing radical production under X-ray radiation. In vitro analysis found that the presence of gold nanowires caused elevated lipid peroxidation and intracellular oxidative stress under radiation. When tested in vivo, gold nanowires plus irradiation led to better tumor suppression than gold nanospheres plus radiation. Moreover, gold nanowires were found to be gradually reduced to shorter nanowires by glutathione, which may benefit fractionated radiation. Conclusion Our studies suggest that gold nanowires are a promising type of radiosensitizer that can be safely injected into tumors to enhance radiotherapy. While the current study was conducted in a breast cancer model, the approach can be extended to the treatment of other cancer types.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

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