BODIPY-containing nanoscale metal–organic frameworks as contrast agents for computed tomography

2017 ◽  
Vol 5 (12) ◽  
pp. 2330-2336 ◽  
Author(s):  
Tao Zhang ◽  
Lei Wang ◽  
Chong Ma ◽  
Weiqi Wang ◽  
Jun Ding ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Contrast Agents ◽  
Metal Organic Frameworks ◽  
Ct Imaging ◽  
Imaging Agent ◽  
Metal Organic

A new computed tomography (CT) imaging agent is developed based on the BODIPY-containing nanoscale metal–organic frameworks (NMOFs). The bio-safety and CT imaging of such NMOFs have been well investigated both in vitro and in vivo.

Tantalum Oxide Nanoparticles as Versatile Contrast Agents for X-ray Computed Tomography

2020 ◽  
Author(s):  
Shatadru Chakravarty ◽  
Jeremy Hix ◽  
Kaitlyn Wieweora ◽  
Maximilian Volk ◽  
Elizabeth Kenyon ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Contrast Agents ◽  
Mesoporous Silica Nanoparticles ◽  
Sol Gel ◽  
Tantalum Oxide ◽  
High Signal ◽  
Drug Delivery Vehicles ◽  
X Ray Computed

Here we describe the synthesis, characterization and in vitro and in vivo performance of a series of tantalum oxide (TaOx) based nanoparticles (NPs) for computed tomography (CT). Five distinct versions of 9-12 nm diameter silane coated TaOx nanocrystals (NCs) were fabricated by a sol-gel method with varying degrees of hydrophilicity and with or without fluorescence, with the highest reported Ta content to date (78%). Highly hydrophilic NCs were left bare and were evaluated in vivo in mice for micro-CT of full body vasculature, where following intravenous injection, TaOx NCs demonstrate high CT contrast, circulation in blood for ~ 3 h, and eventual accumulation in RES organs; and following injection locally in the mammary gland, where the full ductal tree structure can be clearly delineated. Partially hydrophilic NCs were encapsulated within mesoporous silica nanoparticles (MSNPs; TaOx@MSNPs) and hydrophobic NCs were encapsulated within poly(lactic-co-glycolic acid) (PLGA; TaOx@PLGA) NPs, serving as potential CT-imagable drug delivery vehicles. Bolus intramuscular injections of TaOx@PLGA NPs and TaOx@MSNPs to mimic the accumulation of NPs at a tumor site produce high signal enhancement in mice. In vitro studies on bare NCs and formuated NPs demonstrate high cytocompatibility and low dissolution of TaOx. This work solidifies that TaOx-based NPs are versatile contrast agents for CT.

scholarly journals Zr- and Hf-based nanoscale metal–organic frameworks as contrast agents for computed tomography

2012 ◽  
Vol 22 (35) ◽  
pp. 18139 ◽  
Author(s):  
Kathryn E. deKrafft ◽  
William S. Boyle ◽  
Laurel M. Burk ◽  
Otto Z. Zhou ◽  
Wenbin Lin
Keyword(s):  
Computed Tomography ◽  
Contrast Agents ◽  
Metal Organic Frameworks ◽  
Metal Organic

scholarly journals A Simple in vivo Assay Using Amphipods for the Evaluation of Potential Biocompatible Metal-Organic Frameworks

2021 ◽  
Vol 9 ◽  
Author(s):  
Ines Sifaoui ◽  
Idaira Pacheco-Fernández ◽  
José E. Piñero ◽  
Verónica Pino ◽  
Jacob Lorenzo-Morales
Keyword(s):  
Ex Vivo ◽  
Colorimetric Assay ◽  
Metal Organic Frameworks ◽  
Screening Method ◽  
Toxicity Tests ◽  
Toxicity Screening ◽  
Metal Organic ◽  
Potential Use

In this study, the application of amphipods in vivo assays was evaluated. The main aim of this work was to check the potential use of this model in biocompatibility assessments of metal-organic frameworks (MOFs). Hence, six different MOFs were synthesized and the in vitro and ex vivo cytotoxicity was first assessed using a colorimetric assay and a macrophage cell line. Obtained results were compared to validate the in vivo toxicity tests carried out using amphipods and increasing concentrations of the different MOFs. Amphipods do not require the need of ethics approval and also are less expensive to keep than conventional in vivo models, showing its potential as a fast and reliable platform in toxicity studies. The obtained results showed that the amphipods based-assay was simple, easy to replicate and yielded toxicity data corresponding to the type of MOFs tested. In addition, it was observed that only CIM-80(Al) and CIM-84(Zr) did not show any toxicity to the animals at the different tested concentrations. Therefore, the developed in vivo model could be applied as a high-throughput toxicity screening method to evaluate the toxicity of numerous materials, chemicals and therapeutic agents among others.

Tantalum Oxide Nanoparticles as Versatile Contrast Agents for X-ray Computed Tomography

2020 ◽  
Author(s):  
Shatadru Chakravarty ◽  
Jeremy Hix ◽  
Kaitlyn Wieweora ◽  
Maximilian Volk ◽  
Elizabeth Kenyon ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Contrast Agents ◽  
Mesoporous Silica Nanoparticles ◽  
Sol Gel ◽  
Tantalum Oxide ◽  
High Signal ◽  
Drug Delivery Vehicles ◽  
X Ray Computed

Here we describe the synthesis, characterization and in vitro and in vivo performance of a series of tantalum oxide (TaOx) based nanoparticles (NPs) for computed tomography (CT). Five distinct versions of 9-12 nm diameter silane coated TaOx nanocrystals (NCs) were fabricated by a sol-gel method with varying degrees of hydrophilicity and with or without fluorescence, with the highest reported Ta content to date (78%). Highly hydrophilic NCs were left bare and were evaluated in vivo in mice for micro-CT of full body vasculature, where following intravenous injection, TaOx NCs demonstrate high CT contrast, circulation in blood for ~ 3 h, and eventual accumulation in RES organs; and following injection locally in the mammary gland, where the full ductal tree structure can be clearly delineated. Partially hydrophilic NCs were encapsulated within mesoporous silica nanoparticles (MSNPs; TaOx@MSNPs) and hydrophobic NCs were encapsulated within poly(lactic-co-glycolic acid) (PLGA; TaOx@PLGA) NPs, serving as potential CT-imagable drug delivery vehicles. Bolus intramuscular injections of TaOx@PLGA NPs and TaOx@MSNPs to mimic the accumulation of NPs at a tumor site produce high signal enhancement in mice. In vitro studies on bare NCs and formuated NPs demonstrate high cytocompatibility and low dissolution of TaOx. This work solidifies that TaOx-based NPs are versatile contrast agents for CT.

Abstract MP207: A Precision Medicine Approach For Non-invasive, Longitudinal, And Quantitative Monitoring Of Cardiac Tissue-engineered Scaffolds

2021 ◽  
Vol 129 (Suppl_1) ◽  
Author(s):  
Carmen Gil ◽  
Connor Evans ◽  
Lan Li ◽  
Merlyn Vargas ◽  
Gabriella Kabboul ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Precision Medicine ◽  
Cardiac Tissue ◽  
Ct Imaging ◽  
3D Bioprinting ◽  
Au Nps ◽  
Non Invasive ◽  
Functional Features

3D bioprinting has revolutionized personalized and precision medicine by enabling the manufacturing of tissue constructs that precisely recapitulate the cellular and functional features of native tissues. In cardiac regenerative medicine, printed scaffolds have shown tremendous potential in repairing damaged heart, however, their clinical applications have been limited by the lack of precise noninvasive tools to monitor the patch function following implantation. By integrating state-of-the-art 3D bioprinting and photon-counting computed tomography (PCCT), this study introduces a new approach for bioengineering defect-specific scaffolds and monitoring their function. We prepared distinct CT-visible bioinks containing a variety of molecular or nanoparticle (NP) contrast agents, including iodine and gadolinium molecules, Au NPs, Gd 2 O 3 NPs, and iodine-loaded liposomes ( Fig 1A-B ). In vitro release experiments showed relatively rapid diffusion-controlled depletion of molecular contrast agents from scaffolds. In contrast, NP agents showed more stable encapsulation and only a partial, degradation-mediated release for up to 3 weeks of incubation ( Fig 1C-D ). Next, PCCT imaging was performed on various scaffold geometries printed using bioinks laden with Gd 2 O 3 or Au NPs. Results demonstrated CT visibility with differential contrast between different patch regions that corresponded to the designed geometries ( Fig 1E ). Finally, we evaluated the in vivo CT imaging of bioprinted patches after their subcutaneous implantation in a mouse model. CT images demonstrated adequate signal from implanted grafts ( Fig 1F ). Together, these results establish a novel precision medicine platform for non-invasive monitoring of medical devices which can open new prospects for a broad range of tissue engineering applications. Figure 1. 3D Bioprinting of CT-visible cardiac patches. A-B: Design of bioinks functionalized with molecular (left) and nanoparticle (right) CT contrast agents ( A ) and their bioprinting ( B ). C-D: In vitro release of contrast agents from printed patches. E: CAD design (left), CT image (middle), and PCCT material decomposition (right) for multi-contrast bioprinted scaffolds. F: In vivo CT imaging of printed patch, laden with Au NPs, implanted subcutaneously into a mouse torso.

scholarly journals Correction: BODIPY-containing nanoscale metal–organic frameworks as contrast agents for computed tomography

2020 ◽  
Vol 8 (48) ◽  
pp. 11107-11108
Author(s):  
Tao Zhang ◽  
Lei Wang ◽  
Chong Ma ◽  
Weiqi Wang ◽  
Jun Ding ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Contrast Agents ◽  
Metal Organic Frameworks ◽  
Metal Organic

Correction for ‘BODIPY-containing nanoscale metal–organic frameworks as contrast agents for computed tomography’ by Tao Zhang et al., J. Mater. Chem. B, 2017, 5, 2330–2336, DOI: 10.1039/C7TB00392G.

Encapsulating doxorubicin in PEGylation metal-organic frameworks for combined radiation therapy in liver cancer treatment

2020 ◽  
Vol 10 (8) ◽  
pp. 1197-1203
Author(s):  
Dawei Liu ◽  
Bing Bai ◽  
Yanbin Sun ◽  
Youxin Guo
Keyword(s):  
Radiation Therapy ◽  
Drug Carrier ◽  
Drug Loading ◽  
Metal Organic Frameworks ◽  
Diagnosis And Treatment ◽  
X Rays ◽  
Chemotherapy Drugs ◽  
Metal Organic

It is well known that nanoscale metal-organic frameworks (NMOFs) have exhibited brilliant advantages in cancer diagnosis and treatment due to their structural diversity, high intrinsic biodegradability, and drug-loading capacities. Herein, based on our previous results, hypoxia was suppressed by inhibiting the expression of the CA IX protein, produced from Zr-MOF nanoparticles, and enhancing radiation therapy (RT) effectiveness. We designed a nanocomposite by encapsulating doxorubicin into Zr-MOF nanoparticles. It is a highly effective drug for chemotherapy and MOF that can absorb X-rays well, applied to enhance RT as the radiosensitizer. PEG is used for surface modification in the nanoparticles and to improve circulation time by intravenous administration. These nanoparticles could be applied for RT combing with chemotherapy in vitro and vivo, obtaining excellent anticancer efficacy. Most importantly, Zr-MOF@Dox demonstrates high clearance from HepG2 tumor cells, reducing the circulation of toxicity in vivo. Our research exhibits a new approach to establishing Zr-MOF@Dox as a biodegradable drug-carrier system, containing chemotherapy drugs and functional elements that totally perform the diagnosis and treatment roles of RT.

Methotrexate-loaded metal-organic frameworks on the basis of γ-cyclodextrin: Design, characterization, in vitro and in vivo investigation

2020 ◽  
Vol 111 ◽  
pp. 110774 ◽  
Author(s):  
Iliya Kritskiy ◽  
Tatyana Volkova ◽  
Tatyana Sapozhnikova ◽  
Anton Mazur ◽  
Peter Tolstoy ◽  
...  
Keyword(s):  
Metal Organic Frameworks ◽  
Metal Organic
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