Living cell intracellular temperature imaging with biocompatible dye-conjugated carbon dots

2017 ◽  
Vol 5 (18) ◽  
pp. 3383-3390 ◽  
Author(s):  
Lin Wei ◽  
Yanhong Ma ◽  
Xiaoya Shi ◽  
Yaxin Wang ◽  
Xin Su ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Living Cell ◽  
Living Cells ◽  
Local Temperature ◽  
Biological Functions ◽  
Temperature Imaging ◽  
Subcellular Organelles

Within living cells, the biological functions of subcellular organelles are highly dependent on the distribution of local temperature.

Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fusobacterium Nucleatum ◽  
Living Cells ◽  
First Time ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

scholarly journals Nanoarchitectonics on living cells

RSC Advances ◽  
2021 ◽  
Vol 11 (31) ◽  
pp. 18898-18914
Author(s):  
Katsuhiko Ariga ◽  
Rawil Fakhrullin
Keyword(s):  
Living Cell ◽  
Living Cells ◽  
Material Surfaces ◽  
As If

We can introduce functional structures with various components on a living cell as if architectures were constructed on material surfaces.

Probing nucleus-enriched proteins in single living cells via subcellular-resolved plasmonic immunosandwich assay

The Analyst ◽  
2021 ◽  
Author(s):  
Jia Liu ◽  
Dan Xie ◽  
Zhen Liu
Keyword(s):  
Living Cells ◽  
Nuclear Proteins ◽  
Biological Functions ◽  
Abnormal Expression ◽  
Single Living Cells ◽  
Single Living ◽  
In Cells

Nuclear proteins are crucial in cells and are greatly linked to various biological functions. Abnormal expression of nuclear proteins is associated with many diseases ranging from inflammation to cancer. However,...

scholarly journals Femtosecond Laser Manipulation of Subcellular Organelles in Living Cells

2005 ◽  
Author(s):  
Wataru Watanabe ◽  
Timoko Shimada ◽  
Kazuyoshi Itoh ◽  
Sachihiro Matsunaga ◽  
Kiichi Fukui
Keyword(s):  
Femtosecond Laser ◽  
Living Cells ◽  
Subcellular Organelles ◽  
Laser Manipulation

scholarly journals Cockayne Syndrome B protein selectively interacts and resolves intermolecular DNA G-quadruplex structures

2021 ◽  
Author(s):  
Denise Liano ◽  
Marco Di Antonio
Keyword(s):  
Ribosomal Dna ◽  
Living Cells ◽  
Cockayne Syndrome ◽  
Biological Functions ◽  
Loss Of Function ◽  
Endogenous Protein ◽  
G Quadruplex ◽  
Dna Strands ◽  
Low Probability ◽  
Dna Strand

AbstractGuanine-rich DNA can fold into secondary structures known as G-quadruplexes (G4s). G4s can form from a single DNA-strand (intramolecular) or from multiple DNA-strands (intermolecular), but studies on their biological functions have been often limited to intramolecular G4s, owing to the low probability of intermolecular G4s to form within genomic DNA. Herein, we report that the endogenous protein Cockayne Syndrome B (CSB) binds with picomolar affinity to intermolecular G4s, whilst displaying negligible binding towards intramolecular structures. We also observed that CSB can selectively resolve intermolecular G4s in an ATP independent fashion. Our study demonstrates that intermolecular G4s formed within ribosomal DNA are natural substrates for CSB, strongly suggesting that these structures might be formed in the nucleolus of living cells. Given that CSB loss of function elicits premature ageing phenotypes, our findings indicate that the interaction between CSB and ribosomal DNA intermolecular G4s is essential to maintain cellular homeostasis.

Graphene-Semiconductor Tubular Needles for Operations of Living Cells

2010 ◽  
Vol 5 (1) ◽  
pp. 90-96
Author(s):  
Aleksandr V. Kopylov ◽  
Viktor Ya. Prinz
Keyword(s):  
High Precision ◽  
Biological Material ◽  
Living Cell ◽  
Cylindrical Shells ◽  
Living Cells ◽  
The Novel ◽  
Graphene Structures

The possibility of application of the novel class of tubular needles for piercing cells and injecting biological material inside the cell is considered. Stability calculations of tubular (multiwall) needles were made. Calculations were made for the needles with walls formed from hybrid graphene-semiconductor or graphene structures and spires shaped as trapeziform open cylindrical shells. The possibility of mass fabrication of such needles and chips for AFM significantly broadens the range of available operations on the surface and inside the living cell and opens prospects of effective high-precision manipulations with individual cells.

Computational Foundations of the Anticipatory Artificial Autopoietic Cellular Automata

2021 ◽  
pp. 289-307
Author(s):  
Daniel M. Dubois ◽  
Stig C. Holmberg
Keyword(s):  
Artificial Intelligence ◽  
Cellular Automata ◽  
Living Cell ◽  
Artificial Life ◽  
Initial Conditions ◽  
Living Cells ◽  
Asymmetric Membrane

A survey of the Varela automata of autopoiesis is presented. The computation of the Varela program, with initial conditions given by a living cell, is not able to self-maintain the membrane of the living cell. In this chapter, the concept of anticipatory artificial autopoiesis (AAA) is introduced. In this chapter, the authors present a new algorithm of the anticipatory artificial autopoiesis, which extend the Varela automata. The main enhancement consists in defining an asymmetric membrane of the artificial lining cell. The simulations show the anticipatory generation of artificial living cells starting with any initial conditions. The new concept of anticipatory artificial autopoiesis is related to artificial life (Alife) and artificial intelligence (AI). This is a breakthrough in the computational foundation of autopoiesis.

scholarly journals Nanorheology of living cells measured by AFM-based force–distance curves

Nanoscale ◽  
2020 ◽  
Vol 12 (16) ◽  
pp. 9133-9143 ◽  
Author(s):  
Pablo D. Garcia ◽  
Carlos R. Guerrero ◽  
Ricardo Garcia
Keyword(s):  
Living Cell ◽  
Viscoelastic Properties ◽  
Living Cells

Method to measure the viscoelastic properties of a living cell by AFM-based force–distance curves.

Intrinsic lysosomal targeting fluorescent carbon dots with ultrastability for long-term lysosome imaging

2020 ◽  
Vol 8 (4) ◽  
pp. 736-742 ◽  
Author(s):  
Shuo Guo ◽  
Yuanqiang Sun ◽  
Xin Geng ◽  
Ran Yang ◽  
Lehui Xiao ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Living Cells ◽  
Apoptotic Cells ◽  
Drug Induced ◽  
Lysosomal Targeting ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Intrinsic lysosomal targeting carbon dots were synthesized with ultrastability for long-term lysosome imaging of living cells and drug-induced apoptotic cells.

Disulfide bond-based self-crosslinked carbon-dots for turn-on fluorescence imaging of GSH in living cells

The Analyst ◽  
2020 ◽  
Vol 145 (8) ◽  
pp. 2982-2987 ◽  
Author(s):  
Jia Li ◽  
Yuhui Wang ◽  
Shan Sun ◽  
A-Man Lv ◽  
Kai Jiang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Disulfide Bond ◽  
Carbon Dots ◽  
Living Cells ◽  
Turn On

A self-quenched nanoprobe built on a disulfide bond-based crosslink of carbon-dots has been constructed for intracellular GSH sensing.

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