scholarly journals Michael addition-based probes for ratiometric fluorescence imaging of protein S-depalmitoylases in live cells and tissues

2017 ◽  
Vol 8 (11) ◽  
pp. 7588-7592 ◽  
Author(s):  
Michael W. Beck ◽  
Rahul S. Kathayat ◽  
Candace M. Cham ◽  
Eugene B. Chang ◽  
Bryan C. Dickinson
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescent Probes ◽  
Michael Addition ◽  
Enzyme Activities ◽  
Protein S ◽  
Live Cell ◽  
Tissue Imaging ◽  
Live Cells ◽  
Ratiometric Fluorescence

Ratiometric fluorescent probes for cysteine palmitoylation “erasers” permit live cell and tissue imaging of endogenous enzyme activities.

scholarly journals Correction: Michael addition-based probes for ratiometric fluorescence imaging of protein S-depalmitoylases in live cells and tissues

2017 ◽  
Vol 8 (11) ◽  
pp. 7879-7879
Author(s):  
Michael W. Beck ◽  
Rahul S. Kathayat ◽  
Candace M. Cham ◽  
Eugene B. Chang ◽  
Bryan C. Dickinson
Keyword(s):  
Fluorescence Imaging ◽  
Michael Addition ◽  
Protein S ◽  
Live Cells ◽  
Ratiometric Fluorescence

Correction for ‘Michael addition-based probes for ratiometric fluorescence imaging of protein S-depalmitoylases in live cells and tissues’ by Michael W. Beck et al., Chem. Sci., 2017, DOI: 10.1039/c7sc02805a

scholarly journals Fluorescent Probes for Live Cell Thiol Detection

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3575
Author(s):  
Shenggang Wang ◽  
Yue Huang ◽  
Xiangming Guan
Keyword(s):  
Fluorescent Probes ◽  
Biological System ◽  
High Sensitivity ◽  
Intracellular Distribution ◽  
Live Cell ◽  
Live Cells ◽  
High Demand ◽  
Non Invasive

Thiols play vital and irreplaceable roles in the biological system. Abnormality of thiol levels has been linked with various diseases and biological disorders. Thiols are known to distribute unevenly and change dynamically in the biological system. Methods that can determine thiols’ concentration and distribution in live cells are in high demand. In the last two decades, fluorescent probes have emerged as a powerful tool for achieving that goal for the simplicity, high sensitivity, and capability of visualizing the analytes in live cells in a non-invasive way. They also enable the determination of intracellular distribution and dynamitic movement of thiols in the intact native environments. This review focuses on some of the major strategies/mechanisms being used for detecting GSH, Cys/Hcy, and other thiols in live cells via fluorescent probes, and how they are applied at the cellular and subcellular levels. The sensing mechanisms (for GSH and Cys/Hcy) and bio-applications of the probes are illustrated followed by a summary of probes for selectively detecting cellular and subcellular thiols.

Near-infrared ratiometric fluorescence imaging of lysosomal polarity in live cells and in vivo

2021 ◽  
pp. 130397
Author(s):  
Xijuan Chao ◽  
Yan Hu ◽  
Rongjian Liu ◽  
Dejun Huang ◽  
Yingmei Zhang
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Live Cells ◽  
Ratiometric Fluorescence

scholarly journals A 2-aza-Cope reactivity-based platform for ratiometric fluorescence imaging of formaldehyde in living cells

2017 ◽  
Vol 8 (5) ◽  
pp. 4073-4081 ◽  
Author(s):  
Thomas F. Brewer ◽  
Guillermo Burgos-Barragan ◽  
Niek Wit ◽  
Ketan J. Patel ◽  
Christopher J. Chang
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescent Probes ◽  
Visible Region ◽  
Living Cells ◽  
Reactive Species ◽  
Ratiometric Fluorescence

We present the first ratiometric fluorescent probes for formaldehyde, a major reactive species in biology, operating in the visible region.

scholarly journals A Trojan Horse for live-cell super-resolution microscopy

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Gerti Beliu ◽  
Markus Sauer
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescent Probes ◽  
Super Resolution ◽  
Trojan Horse ◽  
Live Cell ◽  
Cell Labeling ◽  
Intracellular Organelles ◽  
Live Cell Labeling ◽  
Super Resolution Microscopy ◽  
The Way

AbstractNew peptide vehicles enable the efficient live-cell labeling of intracellular organelles with cell-impermeable fluorescent probes by simple coincubation, paving the way for refined multicolor super-resolution fluorescence imaging.

scholarly journals A dual-fluorophore sensor approach for ratiometric fluorescence imaging of potassium in living cells

2021 ◽  
Author(s):  
Zeming Wang ◽  
Tyler C. Detomasi ◽  
Christopher J. Chang
Keyword(s):  
Fluorescence Imaging ◽  
Internal Standard ◽  
Living Cells ◽  
Fluorescent Imaging ◽  
Live Cells ◽  
Ratiometric Fluorescence ◽  
Coumarin 343

We report a dual-fluorophore approach for ratiometric fluorescent imaging of K+ levels in live cells. Intracellular esterases cleave RPS-1 to detach the K+-responsive fluorophore (PS525) from its internal standard (Coumarin 343).

scholarly journals Author Correction: Simple and efficient delivery of cell-impermeable organic fluorescent probes into live cells for live-cell superresolution imaging

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Meng Zhang ◽  
Meihua Li ◽  
Wenting Zhang ◽  
Yubing Han ◽  
Yu-Hui Zhang
Keyword(s):  
Fluorescent Probes ◽  
Live Cell ◽  
Live Cells ◽  
Superresolution Imaging ◽  
Efficient Delivery

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

α-Methylene-β-Lactone Probe for Measuring Live-Cell Reactions of Small Molecules

2020 ◽  
Author(s):  
Lei Wang ◽  
Louis Riel ◽  
Bekim Bajrami ◽  
Bin Deng ◽  
Amy Howell ◽  
...  
Keyword(s):  
Mass Spectra ◽  
Live Cell ◽  
Live Cells ◽  
The Novel ◽  
Proteomics Analysis ◽  
Chemical Proteomics ◽  
Tandem Mass Spectra ◽  
Modified Peptides ◽  
Covalent Probes ◽  
Ht 29

The novel use of the α-methylene-β-lactone (MeLac) moiety as a warhead of multiple electrophilic sites is reported. In this study, we demonstrate that a MeLac-alkyne is a competent covalent probe and reacts with diverse proteins in live cells. Proteomics analysis of affinity-enriched samples identifies probe-reacted proteins, resolves their modified peptides/residues, and thus characterizes probe-protein reactions. Unique methods are developed to evaluate confidence in the identification of the reacted proteins and modified peptides. Tandem mass spectra of the peptides reveal that MeLac reacts with nucleophilic cysteine, serine, lysine, threonine, and tyrosine residues, through either Michael addition or acyl addition. A peptide-centric proteomics platform, using MeLac-alkyne as the measurement probe, successfully analyzes the Orlistat selectivity in live HT-29 cells. MeLac is a versatile warhead demonstrating enormous potential to expedite the development of covalent probes and inhibitors in interrogating protein (re)activity. MeLac-empowered platforms in chemical proteomics are widely adaptable for measuring the live-cell action of reactive molecules.

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