scholarly journals Determination of protonation states of iminosugar–enzyme complexes using photoinduced electron transfer

2017 ◽  
Vol 8 (11) ◽  
pp. 7383-7393 ◽  
Author(s):  
Bo Wang ◽  
Jacob Ingemar Olsen ◽  
Bo W. Laursen ◽  
Jens Christian Navarro Poulsen ◽  
Mikael Bols
Keyword(s):  
Electron Transfer ◽  
Active Site ◽  
Photoinduced Electron Transfer ◽  
White Rot ◽  
White Rot Fungus ◽  
Amino Group ◽  
Enzyme Complexes

N-alkylated analogues of 1-deoxynojirimycin inhibit β-glucosidase from white rot fungus. The amino group in the β-glucosidase–iminosugar complex is unprotonated when bound, while an active site carboxylate is protonated.

Novel anthracene- and pyridine-containing Schiff base probe for selective “off–on” fluorescent determination of Cu2+ ions towards live cell application

2016 ◽  
Vol 40 (7) ◽  
pp. 6101-6108 ◽  
Author(s):  
Turibius Simon ◽  
Muthaiah Shellaiah ◽  
Venkatesan Srinivasadesikan ◽  
Ching-Chang Lin ◽  
Fu-Hsiang Ko ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Schiff Base ◽  
Photoinduced Electron Transfer ◽  
Transfer Mechanism ◽  
Live Cell ◽  
Live Cells ◽  
Electron Transfer Mechanism

A simple anthracene-based AP probe was synthesized to detect Cu2+ ions, via the photoinduced electron transfer mechanism, in live cells.

scholarly journals Late-Stage Functionalisation of Polycyclic (N-Hetero-) Aromatic Hydrocarbons by Detoxifying CYP5035S7 Monooxygenase of the White-Rot Fungus Polyporus arcularius

Biomolecules ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1708
Author(s):  
Nico D. Fessner ◽  
Christopher Grimm ◽  
Wolfgang Kroutil ◽  
Anton Glieder
Keyword(s):  
Aromatic Hydrocarbons ◽  
Active Site ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Cytochrome P450 Enzymes ◽  
Factors Influencing ◽  
Steric Factors ◽  
Polycyclic Aromatic ◽  
Insight Into

Functionalisation of polycyclic aromatic hydrocarbons (PAHs) and their N-heteroarene analogues (NPAHs) is a tedious synthetic endeavour that requires diverse bottom-up approaches. Cytochrome P450 enzymes of white-rot fungi were shown to participate in the fungal detoxification of xenobiotics and environmental hazards via hydroxylation of PAH compounds. In this paper, the recently discovered activity of the monooxygenase CYP5035S7 towards (N)PAHs was investigated in detail, and products formed from the substrates azulene, acenaphthene, fluorene, anthracene, and phenanthrene by whole-cell biocatalysis were isolated and characterised. The observed regioselectivity of CYP5035S7 could be explained by a combination of the substrate’s electron density and steric factors influencing the substrate orientation giving insight into the active-site geometry of the enzyme.

The Elusive 5′-Deoxyadenosyl Radical: Captured and Characterized by EPR and ENDOR Spectroscopies

2019 ◽  
Author(s):  
Hao Yang ◽  
Elizabeth C. McDaniel ◽  
Stella Impano ◽  
Amanda S. Byer ◽  
Richard Jodts ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Active Site ◽  
Photoinduced Electron Transfer ◽  
Bond Cleavage ◽  
Biological Role ◽  
Report Generation ◽  
Half Century ◽  
Enzyme Active Site ◽  
Novel Approach

The 5'-deoxyadenosyl radical (5'-dAdo•) abstracts a substrate H-atom as the first step in radical-based transformations catalyzed by adenosylcobalamin-dependent and radical <i>S</i>-adenosyl-l-methionine (RS) enzymes. Notwithstanding its central biological role, 5'-dAdo• has eluded characterization despite efforts spanning more than a half-century. Here we report generation of 5'-dAdo• in a RS enzyme active site at 12 K, using a novel approach involving cryogenic photoinduced electron transfer from the [4Fe-4S]<sup>+</sup>cluster to the coordinated <i>S</i>-adenosylmethionine (SAM) to induce homolytic S-C' bond cleavage.

The Elusive 5′-Deoxyadenosyl Radical: Captured and Characterized by EPR and ENDOR Spectroscopies

2019 ◽  
Author(s):  
Hao Yang ◽  
Elizabeth C. McDaniel ◽  
Stella Impano ◽  
Amanda S. Byer ◽  
Richard Jodts ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Active Site ◽  
Photoinduced Electron Transfer ◽  
Bond Cleavage ◽  
Biological Role ◽  
Report Generation ◽  
Half Century ◽  
Enzyme Active Site ◽  
Novel Approach

The 5'-deoxyadenosyl radical (5'-dAdo•) abstracts a substrate H-atom as the first step in radical-based transformations catalyzed by adenosylcobalamin-dependent and radical <i>S</i>-adenosyl-l-methionine (RS) enzymes. Notwithstanding its central biological role, 5'-dAdo• has eluded characterization despite efforts spanning more than a half-century. Here we report generation of 5'-dAdo• in a RS enzyme active site at 12 K, using a novel approach involving cryogenic photoinduced electron transfer from the [4Fe-4S]<sup>+</sup>cluster to the coordinated <i>S</i>-adenosylmethionine (SAM) to induce homolytic S-C' bond cleavage.

scholarly journals Single electron transfer by an extracellular laccase from the white-rot fungus Pleurotus ostreatus

Microbiology ◽  
1995 ◽  
Vol 141 (2) ◽  
pp. 393-398 ◽  
Author(s):  
H.-D. Youn ◽  
K.-J. Kim ◽  
J.-S. Maeng ◽  
Y.-H. Han ◽  
I.-B. Jeong ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Pleurotus Ostreatus ◽  
Single Electron ◽  
White Rot ◽  
White Rot Fungus ◽  
Single Electron Transfer ◽  
Extracellular Laccase
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