scholarly journals Thiol–ene photoimmobilization of chymotrypsin on polysiloxane gels for enzymatic peptide synthesis

RSC Advances ◽  
2018 ◽  
Vol 8 (22) ◽  
pp. 11843-11849
Author(s):  
Meng Wang ◽  
Jun Xing ◽  
Yu-Tang Sun ◽  
Ling-Xiang Guo ◽  
Bao-Ping Lin ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Catalytic Activity ◽  
Click Chemistry ◽  
Peptide Synthesis ◽  
Enzymatic Peptide Synthesis

Here we report a strategy to photoimmobilize chymotrypsin onto polysiloxane gels via thiol-ene click chemistry. The polysiloxane-immobilized chymotrypsin shows enhanced catalytic activity, thermal stability and recyclability.

Catalytic activity of α-chymotrypsin in enzymatic peptide synthesis in ionic liquids

2009 ◽  
Vol 47 (1-3) ◽  
pp. 27-30 ◽  
Author(s):  
Hidetaka Noritomi ◽  
Katsuyuki Suzuki ◽  
Manabu Kikuta ◽  
Satoru Kato
Keyword(s):  
Catalytic Activity ◽  
Ionic Liquids ◽  
Peptide Synthesis ◽  
Enzymatic Peptide Synthesis

Catalytic activity of niobium oxynitride and carbide I. Preparation, characterization and thermal stability of finely divided niobium oxynitrides

1994 ◽  
Vol 119 (2) ◽  
pp. 223-240 ◽  
Author(s):  
Hak Soo Kim ◽  
Chae Ho Shin ◽  
Guy Bugli ◽  
Monique Bureau-Tardy ◽  
Gerald Djega-Mariadassou
Keyword(s):  
Thermal Stability ◽  
Catalytic Activity ◽  
Thermal Stability Of

scholarly journals Influence of Water Activity on Protease Adsorbed on Biochar in Organic Solvents

2017 ◽  
Vol 6 (4) ◽  
pp. 96 ◽  
Author(s):  
Hidetaka Noritomi ◽  
Jumpei Nishigami ◽  
Nobuyuki Endo ◽  
Satoru Kato ◽  
Katsumi Uchiyama
Keyword(s):  
Thermal Stability ◽  
Catalytic Activity ◽  
Organic Solvents ◽  
Water Activity ◽  
Initial Rate ◽  
Butyl Ester ◽  
Nitrogen Atmosphere ◽  
Bamboo Charcoal ◽  
Influence Of Water ◽  
Hydrolysis Of

We have found that the organic solvent-resistance of Alpha-chymotrypsin (Alpha-CT) is enhanced by adsorbing Alpha-CT onto bamboo charcoal powder (BCP), which is obtained by pyrolyzing bamboo waste under nitrogen atmosphere, and is markedly dependent on the thermodynamic water activity (aw) in organic solvents. When BCP-adsorbed　Alpha-CT was immersed in acetonitrile at an appropriate water activity, it effectively enhanced the transesterification of N-acetyl-L-tyrosine ethyl ester (N-Ac-Tyr-OEt) with n-butanol (BuOH) to produce N-acetyl-L-tyrosine butyl ester (N-Ac-Tyr-OBu), compared to the hydrolysis of N-Ac-Tyr-OEt with water to give N-acetyl-L-tyrosine (N-Ac-Tyr-OH). When the water activity was 0.28, the initial rate of transesterification catalyzed by BCP-adsorbed Alpha-CT was about sixty times greater than that catalyzed by free Alpha-CT. Regarding the reaction selectivity which is defined as a ratio of the initial rate of transesterification to that of hydrolysis, BCP-adsorbed α-CT was much superior to free Alpha-CT. The catalytic activity of BCP-adsorbed Alpha-CT was markedly dependent on the reaction temperature. Furthermore, concerning the thermal stability at 50 oC, the half-life of BCP-adsorbed Alpha-CT exhibited 3.8-fold, compared to that of free Alpha-CT.

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