scholarly journals A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

RSC Advances ◽  
2018 ◽  
Vol 8 (5) ◽  
pp. 2418-2425 ◽  
Author(s):  
Haibo Xing ◽  
Wenchao Gu ◽  
Dang Xu ◽  
Fuxiang Tian ◽  
Linyun Yao ◽  
...  

A rapid biosensor for the detection of cyromazine in milk is reported based on a fluorescence quenching result.

RSC Advances ◽  
2013 ◽  
Vol 3 (38) ◽  
pp. 16962 ◽  
Author(s):  
Shenshan Zhan ◽  
Yuangen Wu ◽  
Le Liu ◽  
Haibo Xing ◽  
Lan He ◽  
...  

2019 ◽  
Vol 72 (7) ◽  
pp. 555
Author(s):  
Xu Dang ◽  
Wenchao Gu ◽  
Xiyin Zheng ◽  
Xuelian Fei ◽  
Fuxiang Tian ◽  
...  

Herein, a rapid, facile, and colourimetric sensor for the detection of cyromazine in raw milk is reported using an aptamer based on gold nanoparticles (AuNPs). A sequence-specific aptamer for cyromazine called Tcyr1 is designed to absorb on the surface of AuNPs and electrostatically interacts with poly(diallyldimethylammonium chloride) (PDDA), which prevents AuNPs from aggregating. It can also self-assemble to form a G-quadruplex-CYR complex with cyromazine. Because of its specificity and stability, the introduction of cyromazine in raw milk would influence the protection thus the following cationic polymer could aggregate AuNPs and cause a remarkable change in colour. According to this, the presence of cyromazine can be determined by the naked eye and means of absorbance. This sensor is selective for the detection of cyromazine in raw milk and has a limit of detection of 200 ppb by the naked eye and of 5.8 ppb by spectrophotometer, and has a detection range from 0.1 to 1 ppm.


2013 ◽  
Vol 41 (22) ◽  
pp. 10323-10333 ◽  
Author(s):  
Justin D. Lormand ◽  
Noah Buncher ◽  
Connor T. Murphy ◽  
Parminder Kaur ◽  
Marietta Y. Lee ◽  
...  

FEBS Letters ◽  
2019 ◽  
Vol 593 (22) ◽  
pp. 3149-3161 ◽  
Author(s):  
Priyanka Toshniwal ◽  
Michelle Nguyen ◽  
Aurore Guédin ◽  
Helena Viola ◽  
Diwei Ho ◽  
...  

2021 ◽  
Author(s):  
Masaki Hagihara

Tandem guanine repeat sequences can adopt guanine quadruplex (G-quadruplex) structures and consecutive guanine repeat sequences can potentially afford multiple G-quadruplex structures. By using a reverse transcriptase stop assay and biophysical...


2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Joan Casals ◽  
Júlia Viladoms ◽  
Enrique Pedroso ◽  
Carlos González

We have studied the structure and stability of the cyclic dodecamer d<pGGGTTAGGGTTA>, containing two copies of the human telomeric repeat. In the presence of sodium, NMR data are consistent with a dimeric structure of the molecule in which two cycles self-associate forming a quadruplex with three guanine tetrads connected by edgewise loops. The two macrocycles are arranged in a parallel way, and the dimeric structure exhibits a high melting temperature. These results indicate that cyclization of the phosphodiester chain does not prevent quadruplex formation, although it affects the global topology of the quadruplex.


ACS Omega ◽  
2017 ◽  
Vol 2 (8) ◽  
pp. 4790-4799
Author(s):  
Prachi Agarwala ◽  
Gargi Pal ◽  
Satyaprakash Pandey ◽  
Souvik Maiti

2019 ◽  
Vol 58 (8) ◽  
pp. 2331-2335 ◽  
Author(s):  
Blaž Bakalar ◽  
Brahim Heddi ◽  
Emmanuelle Schmitt ◽  
Yves Mechulam ◽  
Anh Tuân Phan

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