scholarly journals A three-dimensional hydroxyapatite/polyacrylonitrile composite scaffold designed for bone tissue engineering

RSC Advances ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 1730-1736 ◽  
Author(s):  
Shuyi Wu ◽  
Jieda Wang ◽  
Leiyan Zou ◽  
Lin Jin ◽  
Zhenling Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Mineralization Process ◽  
Electrospinning Technique ◽  
Fibrous Scaffold

A fluffy and porous (3D) HA composite fibrous scaffold was fabricated by employing an improved electrospinning technique combined with a bio-mineralization process.

Preparation and Performance Analysis of Mesh Hydroxyapatite/Sodium Alginate Composite Scaffold

2015 ◽  
Vol 1119 ◽  
pp. 239-244
Author(s):  
Yan Xu ◽  
Jian Pin Zhou ◽  
Zheng Ying Wei ◽  
Li Yan Dang ◽  
Feng Lin Wu
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Sodium Alginate ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Three Dimensional Printing ◽  
Artificial Bones ◽  
Key Factor ◽  
And Performance

Scaffolds material is the key factor for bone tissue engineering, and construction of the scaffolds is also an important part. Adopting the biocompatible, biodegradable, hydroxyapatite (HAP) and sodium alginate (SA) as the molding material, using three-dimensional printing technology, choosing cross grid filling paths, we manufactured the artificial bones through self-developed 3D printing equipment. Then we measured and analyzed important parameters of the work, and did composite culture experiment. It can be seen that the prepared artificial bone scaffold has good biocompatibility. The paper provides a reference for the study of bone tissue engineering materials.

scholarly journals Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Hui Fan ◽  
Junfeng Hui ◽  
Zhiguang Duan ◽  
Daidi Fan ◽  
Yu Mi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Mechanical Tests ◽  
Cell Counting ◽  
Cross Linking ◽  
The Cross ◽  
Cell Adhesion And Proliferation

We investigated the feasibility of oleuropein as a cross-linking agent for fabricating three-dimensional (3D) porous composite scaffolds for bone tissue engineering. Human-like collagen (HLC) and nanohydroxyapatite (n-HAp) were used to fabricate the composite scaffold by way of cross-linking. The mechanical tests revealed superior properties for the cross-linked scaffolds compared to the uncross-linked scaffolds. The as-obtained composite scaffold had a 3D porous structure with pores ranging from 120 to 300 μm and a porosity of73.6±2.3%. The cross-linked scaffolds were seeded with MC3T3-E1 Subclone 14 mouse osteoblasts. Fluorescence staining, the Cell Counting Kit-8 (CCK-8) assay, and scanning electron microscopy (SEM) indicated that the scaffolds enhanced cell adhesion and proliferation. Our results indicate the potential of these scaffolds for bone tissue engineering.

Collagen-Inspired Nano-fibrous Poly(L-lactic acid) Scaffolds for Bone Tissue Engineering Created from Reverse Solid Freeform Fabrication

2004 ◽  
Vol 823 ◽  
Author(s):  
Victor J. Chen ◽  
Laura A. Smith ◽  
Peter X. Ma
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Computer Aided Design ◽  
Type I Collagen ◽  
Polymer Concentration ◽  
Three Dimensional ◽  
Type I ◽  
Fibrous Scaffold

AbstractReverse solid freeform (SFF) fabrication was used to create highly-controlled macroporous structures in nano-fibrous poly (L-lactic acid) (PLLA) scaffolds. By using a computer-aided design (CAD) program to create a negative template for the scaffold, the three-dimensional (3-D) mold was created on a 3-D printer using a wax. After the template was printed, a solution of PLLA in tetrahydrofuran (THF) was cast into the mold, and was subsequently phase separated at -70°C which gives the nano-fibrous morphology. This resulted in a 3-D nano-fibrous scaffold with a uniform fiber mesh throughout the entire matrix, and greatly increased the surface area within the scaffold. Fiber diameters in these scaffolds were 50-500 nm, similar to type I collagen, and the densities of the fiber meshes can be altered by changing the polymer concentration. To examine the scaffold's potential for tissue regeneration, MC3T3-E1 osteoblasts were seeded and cultured on the scaffolds. Results show that the osteoblasts attached and proliferated on the scaffolds. After 6 weeks in culture, bone-like tissue was evident within the nano-fibrous scaffolds. By having the ability to control the macroporous architecture, interconnectivity, orientation, and external shape of the scaffold, as well as the nanometer-scaled fibrous features in the pore walls, this SFF fabrication/phase separation technique has great potential to design and create ideal scaffolds for bone tissue engineering.

Preparation and characterisation of a novel polylactic acid/hydroxyapatite/graphene oxide/aspirin drug-loaded biomimetic composite scaffold

2021 ◽  
Author(s):  
Shuqiong Liu ◽  
Wu Xiaoyan ◽  
Jiapeng Hu ◽  
Zhenzeng Wu ◽  
Yuying Zheng
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Polylactic Acid ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Composite Scaffolds ◽  
Biomimetic Scaffolds

Biomimetic scaffolds loaded with drugs can be applied in bone tissue engineering. In this study, a series of three-dimensional polylactic acid/hydroxyapatite/graphene oxide (PLA/HA/GO) drug-loaded biomimetic composite scaffolds with different concentrations...

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

scholarly journals Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
pp. 57-69
Author(s):  
Amirhosein Fathi ◽  
Farzad Kermani ◽  
Aliasghar Behnamghader ◽  
Sara Banijamali ◽  
Masoud Mozafari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

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