scholarly journals Trace determination of chlorpheniramine in human plasma using magnetic dispersive solid-phase extraction based on a graphene oxide/Fe3O4@polythionine nanocomposite combined with high-performance liquid chromatography

RSC Advances ◽  
2017 ◽  
Vol 7 (84) ◽  
pp. 53210-53218 ◽  
Author(s):  
Maryam Ahmadi Daryakenary ◽  
Mohsen Zeeb
Keyword(s):  
High Performance Liquid Chromatography ◽  
Graphene Oxide ◽  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Human Plasma ◽  
High Performance ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction

Graphene oxide/Fe3O4@polythionine (GO/Fe3O4@PTh) nanocomposite was fabricated for magnetic dispersive solid-phase extraction and high-performance liquid chromatography-ultraviolent detection (HPLC) of chlorpheniramine in human plasma.

scholarly journals Separation and Determination of Paraquat and Diquat in Human Plasma and Urine by Magnetic Dispersive Solid Phase Extraction Coupled with High-Performance Liquid Chromatography

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Ou Sha ◽  
Bowen Cui ◽  
Xiaobing Chen ◽  
Hua Liu ◽  
Jiawei Yao ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Human Plasma ◽  
High Performance ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Human Plasma And Urine

A magnetic dispersive solid phase extraction method coupled with high-performance liquid chromatography was proposed for the simultaneous separation and determination of paraquat (PQ) and diquat (DQ) in human plasma and urine samples. Based on the reduction of PQ and DQ to a blue radical and yellow-green radical by sodium dithionite in an alkaline medium, a fast colorimetric method was also developed for the fast detection of PQ or DQ. In this paper, CoFe2O4@SiO2 magnetic nanoparticles were used as the adsorbent for the magnetic dispersive solid phase extraction of paraquat and diquat, and these two analytes were found to be eluted directly from the adsorbent by NaOH solution. The main factors affecting the extraction efficiency including amount of extractant, extraction time, sample volume, sample solution pH, and elution volume were optimized. Under the optimized experimental conditions, the calibration curve was linear at a concentration range of 28.5–570.2 μg/L, and the correlation coefficient of paraquat and diquat was 0.9986 and 0.9980, respectively. The limits of detection of paraquat and diquat were 4.5 μg/L and 4.3 μg/L. The proposed MSPE-HPLC method was successfully applied to the detection of the paraquat and diquat in human plasma and urine with satisfied recoveries of PQ and DQ in the range of 87.5%–98.7%.

scholarly journals Magnetic-Dispersive Solid Phase Extraction Based on Graphene Oxide–Fe3O4 Nanocomposites Followed by High Performance Liquid Chromatography-Fluorescence for the Preconcentration and Determination of Terazosin Hydrochloride in Human Plasma

2019 ◽  
Vol 58 (2) ◽  
pp. 178-186 ◽  
Author(s):  
Yaser Pashaei ◽  
Bahram Daraei ◽  
Maryam Shekarchi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Graphene Oxide ◽  
Human Plasma ◽  
High Performance ◽  
Solid Phase ◽  
Phase Extraction ◽  
Plasma Samples ◽  
Dispersive Solid Phase Extraction ◽  
X Ray

Abstract In the present study, a facile modified impregnation method was employed to synthesize superparamagnetic graphene oxide–Fe3O4 (GO–Fe3O4) nanocomposites. Based on the GO–Fe3O4 as adsorbent, a simple and fast magnetic-dispersive solid phase extraction followed by high performance liquid chromatography with fluorescence detection (M-dSPE–HPLC–FL) method was established and validated for the preconcentration and determination of terazosin hydrochloride (TRZ) in human plasma samples. The obtained nanomaterials were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy and vibrating sample magnetometry. Different parameters affecting the extraction efficiency, such as sample pH, amount of sorbent, extraction time, elution solvent and its volume and desorption time, were evaluated and optimized. The linearity of the proposed method was excellent over the range 0.3–50.0 ng mL−1 with an acceptable coefficient of determination (R2 = 0.9989). The limit of quantification and limit of detection were found to be 0.3 and 0.09 ng mL−1, respectively, and the preconcentration factor of 10 was achieved. Intra- and inter-day precision expressed as relative standard deviation (RSD %, n = 6) were between 2.2–3.8% and 4.7–6.4%, respectively. Accuracy, estimated by recovery assays, was 97.7–106.6% with RSD ≤ 5.2%. Ultimately, the applicability of the method was successfully confirmed by the extraction and determination of TRZ in human plasma samples.

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