scholarly journals Optimization of a minimal synergistic enzyme system for hydrolysis of raw cassava pulp

RSC Advances ◽  
2017 ◽  
Vol 7 (76) ◽  
pp. 48444-48453 ◽  
Author(s):  
Benjarat Bunterngsook ◽  
Thanaporn Laothanachareon ◽  
Suda Natrchalayuth ◽  
Sirithorn Lertphanich ◽  
Tatsuya Fujii ◽  
...  

Cassava pulp is an underused agricultural by-product comprising residual starch granules entrapped in cell wall polysaccharides, making it unique from other lignocellulosic wastes in terms of enzymatic processing.

1995 ◽  
Vol 68 (3) ◽  
pp. 389-394 ◽  
Author(s):  
Janice G Miller ◽  
Callum J Buchanan ◽  
Martin A Eastwood ◽  
Stephen C Fry

Author(s):  
F.B. de Castro ◽  
P.M. Hotten ◽  
E.R. Ørskov

Extensive hydrolysis of cell wall polysaccharides by rumen bacteria or free enzymes has been reported when lignocellulosic materials had been treated with steam and pressure (Dekker & Wallis, 1983; Castro & Machado, 1989). This has mainly been explained by complete hydrolysis of hemicellulose, lignin depolymerization and redistribution within the cell wall and increasing accessible pore volume by swelling of the cell walls. Physical treatment based on use of steam and pressure alone (auto-hydrolysis) is always associated with the release of toxic levels of furfural and phenolic monomers. These chemicals are able to inhibit the activity of rumen microorganisms, yeasts and free enzymes. To overcome this effect, dilute-acid-hydrolysis at low temperatures and pressures has been proposed (Grohmann et al., 1985). The aim of this study was to evaluate the effect of temperature, sulphuric acid concentration and reaction time on the utilization of treated wheat straw by dilute-acid-hydrolysis, either by rumen bacteria and free enzymes.


1960 ◽  
Vol 38 (6) ◽  
pp. 869-881 ◽  
Author(s):  
C. T. Bishop ◽  
F. Blank ◽  
P. E. Gardner

Cells of Candida albicans, a pathogenic yeast, have been shown to contain, in addition to chitin, a glucan ([α]D − 30°) and a mannan ([α]D + 78°) in the approximate ratio of 1.00:0.64. The two polysaccharides were easily distinguishable by moving boundary electrophoresis in borate buffer and were separated from each other by fractionation of their copper complexes. Methylation and hydrolysis of the glucan yielded the following O-methyl ethers of D-glucose: 2,3,4,6-tetra-O-methyl (7 moles); 2,3,4-tri-O-methyl (13 moles); 2,4,6-tri-O-methyl (trace); 2,4-di-O-methyl (6 moles); and 2-O-methyl (1 mole). It was concluded that the glucan was a highly branched polysaccharide containing β 1 → 6 and β 1 → 3 linked residues. Periodate oxidation of the glucan supported this conclusion.Methylation and hydrolysis of the mannan yielded the following O-methyl ethers of D-mannose: 2,3,4,6-tetra-O-methyl (1.65 moles); 3,4,6-tri-O-methyl (1.00 mole); 2,3,6-tri-O-methyl (0.18 mole); 3,4-di-O-methyl (1.90 moles). The mannan was therefore a highly branched polysaccharide with short chains of α 1 → 2 linked mannose residues joined together by α 1 → 6 linkages. Results of periodate oxidation agreed with this structure.The differences between these two polysaccharides and glucans and mannans found in other yeasts are discussed.


IAWA Journal ◽  
1983 ◽  
Vol 4 (2-3) ◽  
pp. 89-101 ◽  
Author(s):  
A. M. Catesson

The deposition of cell wall polysaccharides and subsequent lignin incrustation were followed during vessel differentiation in carnation stems. In the secondary walls, lignin deposition was closely accompanied by an apparent loss of free vic-glycol groups from polysaccharides. This apparent loss could be related to the progressive binding of lignin molecules to cell wall carbohydrates. The intensity of lignification in mature intervessel primary walls exhibited a positive gradient from the earliest- to the latestformed metaxylem elements. Intervessel pitmembrane hydrolysis appeared as a sequential and ordered process. A partial hydrolysis of the vessel primary wall was sometimes observed in vessel-to-parenchyma pits.


Sign in / Sign up

Export Citation Format

Share Document