scholarly journals A highly sensitive electrochemical IFN-γ aptasensor based on a hierarchical graphene/AuNPs electrode interface with a dual enzyme-assisted amplification strategy

RSC Advances ◽  
2017 ◽  
Vol 7 (71) ◽  
pp. 45053-45060 ◽  
Author(s):  
Yu Yin ◽  
Lei Shi ◽  
Zhenyu Chu ◽  
Wanqin Jin
Keyword(s):  
Modified Electrode ◽  
Signal Amplification ◽  
Electrochemical Aptasensor ◽  
Highly Sensitive ◽  
Electrode Interface ◽  
Ifn Γ ◽  
Amplification Strategy

A new signal-on electrochemical aptasensor for IFN-γ assay is constructed on a hierarchical graphene/AuNPs modified electrode coupled with a dual enzyme-assisted signal amplification strategy.

An electrochemical aptasensor for detection of IFN-γ using graphene and a dual signal amplification strategy based on the exonuclease-mediated surface-initiated enzymatic polymerization

The Analyst ◽  
2015 ◽  
Vol 140 (22) ◽  
pp. 7784-7791 ◽  
Author(s):  
Chang Liu ◽  
Guiming Xiang ◽  
Dongneng Jiang ◽  
Linlin Liu ◽  
Fei Liu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Enzymatic Polymerization ◽  
Electrochemical Aptasensor ◽  
Target Recycling ◽  
Ifn Γ ◽  
Amplification Strategy ◽  
Dual Signal Amplification

A novel aptasensor based on exonuclease-catalyzed target recycling and surface-initiated enzymatic polymerization for amplification.

A highly sensitive electrochemical aptasensor for detection of microcystin-LR based on a dual signal amplification strategy

The Analyst ◽  
2019 ◽  
Vol 144 (5) ◽  
pp. 1671-1678 ◽  
Author(s):  
Xiaoqiang Liu ◽  
Yunfei Tang ◽  
Peipei Liu ◽  
Liwei Yang ◽  
Lele Li ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Signal Amplification ◽  
Electrochemical Aptasensor ◽  
Ternary Composite ◽  
Highly Sensitive ◽  
Amplification System ◽  
Amplification Strategy ◽  
Dual Signal Amplification ◽  
Signal Amplification System

In this work, a sensitive and selective electrochemical aptasensor for detection of microcystin-LR was developed based on a dual signal amplification system consisted of a novel ternary composite and horseradish peroxidase.

A highly sensitive electrochemical microRNA-21 biosensor based on intercalating methylene blue signal amplification and a highly dispersed gold nanoparticles/graphene/polypyrrole composite

The Analyst ◽  
2021 ◽  
Vol 146 (8) ◽  
pp. 2679-2688
Author(s):  
Chammari Pothipor ◽  
Noppadol Aroonyadet ◽  
Suwussa Bamrungsap ◽  
Jaroon Jakmunee ◽  
Kontad Ounnunkad
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Highly Sensitive ◽  
Highly Dispersed ◽  
Polypyrrole Composite ◽  
Amplification Strategy ◽  
Dispersed Gold ◽  
Potential Tool

An ultrasensitive electrochemical biosensor based on a gold nanoparticles/graphene/polypyrrole composite modified electrode and a signal amplification strategy employing methylene blue is developed as a potential tool for the detection of miRNA-21.

An electrochemical aptasensor based on cocoon-like DNA nanostructure signal amplification for the detection of Escherichia coli O157:H7

The Analyst ◽  
2020 ◽  
Vol 145 (22) ◽  
pp. 7340-7348
Author(s):  
Huasong Bai ◽  
Shengjun Bu ◽  
Wensen Liu ◽  
Chengyu Wang ◽  
Zhongyi Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Signal Amplification ◽  
Selective Detection ◽  
Escherichia Coli O157 ◽  
Dna Nanostructures ◽  
Electrochemical Aptasensor ◽  
Dna Nanostructure ◽  
Highly Sensitive

We developed an electrochemical aptasensor based on cocoon-like DNA nanostructures as signal tags for highly sensitive and selective detection of Escherichia coli O157:H7.

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

BMC Chemistry ◽  
2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3′ end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3′–5′ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0 × 10−3–1000 ng mL−1 with a low limit of detection of 0.15 pg mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

Homogeneous electrochemical aptasensor based on a dual amplification strategy for sensitive detection of profenofos residues

2018 ◽  
Vol 42 (17) ◽  
pp. 14642-14647 ◽  
Author(s):  
Yancui Jiao ◽  
Jiayun Fu ◽  
Wenjie Hou ◽  
Zhaoqiang Shi ◽  
Yemin Guo ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Homogeneous Type ◽  
Electrochemical Aptasensor ◽  
Dual Amplification ◽  
Amplification Strategy

A homogeneous type of electrochemical aptasensor was designed based upon the principle of target-induced and tool enzyme-assisted signal amplification, which was employed for the detection of profenofos residues.

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