scholarly journals A microfluidic model of the blood–brain barrier to study permeabilization by pulsed electric fields

RSC Advances ◽  
2017 ◽  
Vol 7 (68) ◽  
pp. 42811-42818 ◽  
Author(s):  
M. Bonakdar ◽  
P. M. Graybill ◽  
R. V. Davalos
Keyword(s):  
Real Time ◽  
Blood Brain Barrier ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Post Treatment ◽  
Brain Barrier ◽  
Real Time Monitoring ◽  
Cell Monolayers ◽  
Blood Brain

This device enables real-time monitoring of permeability across cell monolayers pre- and post-treatment by pulsed electric fields.

scholarly journals Real-time monitoring of human blood-brain barrier disruption

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0174072 ◽  
Author(s):  
Vesa Kiviniemi ◽  
Vesa Korhonen ◽  
Jukka Kortelainen ◽  
Seppo Rytky ◽  
Tuija Keinänen ◽  
...  
Keyword(s):  
Real Time ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
Brain Barrier ◽  
Real Time Monitoring ◽  
Barrier Disruption ◽  
Blood Brain Barrier Disruption ◽  
Blood Brain ◽  
Brain Barrier Disruption

scholarly journals Real-Time MRI Guidance for Reproducible Hyperosmolar Opening of the Blood-Brain Barrier in Mice

2018 ◽  
Vol 9 ◽  
Author(s):  
Chengyan Chu ◽  
Guanshu Liu ◽  
Miroslaw Janowski ◽  
Jeff W. M. Bulte ◽  
Shen Li ◽  
...  
Keyword(s):  
Real Time ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Mri Guidance ◽  
Blood Brain

scholarly journals Real-Time, Transcranial Monitoring of Safe Blood-Brain Barrier Opening in Non-Human Primates

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e84310 ◽  
Author(s):  
Fabrice Marquet ◽  
Tobias Teichert ◽  
Shih-Ying Wu ◽  
Yao-Sheng Tung ◽  
Matthew Downs ◽  
...  
Keyword(s):  
Real Time ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Blood Brain ◽  
Barrier Opening ◽  
Safe Blood ◽  
Blood Brain Barrier Opening

scholarly journals Targeting blood-brain barrier leakage for visualizing , monitoring, and evaluating ischemia reperfusion injury with Rus-Tc99m Probe

2020 ◽  
Author(s):  
Shuaishuai Gong ◽  
Jieman Wang ◽  
Zhuo Chen ◽  
Xuewei Pan ◽  
Yunhao Wu ◽  
...  
Keyword(s):  
Real Time ◽  
Blood Brain Barrier ◽  
Ischemia Reperfusion Injury ◽  
Protective Efficacy ◽  
Ischemia Reperfusion ◽  
In Vitro Study ◽  
Brain Barrier ◽  
Blue Staining ◽  
Blood Brain

Abstract Background : Cerebral ischemia-reperfusion (I/R) injury as a serious threat to human health is characterized by cerebral endothelial leakage, as a result of the damage of blood-brain barrier (BBB). It is thus quite attractive to realize real-time monitoring of BBB damage for therapeutic surveillance.Methods : In this study, a radioactive probe is constructed by conjugating ruscogenin (Rus), a neuroprotectants, to technetium-99m (Tc 99m ) to assess the damage of cerebral endothelial in BBB.Results : In vitro study proves that the probe can penetrate more efficiently in damaged BBB. Then, longitudinal nuclear imaging distinguishes mice with BBB leakage from normal ones, which is validated by evans blue staining of brain tissue. Higher nuclear signal also correlates with poorer blood circulation in brain. Further, by visualizing brain signal during drug treatment, the probe finds that the most obvious protective efficacy of Rus occurs at 12 h post administration, which is superior than edaravone (Edara).Conclusion : Altogether, the probe is promising to monitor I/R injury real-time by radioactive-imaging of BBB integrity. Importantly, Rus as a neuroprotectants may serve as a potential theranostic agent for I/R treatment.

Disruption of the Blood-Brain Barrier by Extracellular Vesicles From Preeclampsia Plasma and Hypoxic Placentae: Attenuation by Magnesium Sulfate

Hypertension ◽  
2021 ◽  
Author(s):  
José León ◽  
Jesenia Acurio ◽  
Lina Bergman ◽  
Juán López ◽  
Anna Karin Wikström ◽  
...  
Keyword(s):  
Magnesium Sulfate ◽  
Blood Brain Barrier ◽  
Extracellular Vesicles ◽  
Normal Pregnancy ◽  
Brain Barrier ◽  
Cell Monolayers ◽  
Cerebrovascular Complications ◽  
Blood Brain

Preeclampsia, a pregnancy-related endothelial disorder, is associated with both cardiovascular and cerebrovascular complications. Preeclampsia requires the presence of a placenta as part of its pathophysiology, yet the role of this organ in the cerebrovascular complications remains unclear. Research has shown that circulating small extracellular vesicles (also known as exosomes) present in preeclampsia plasma can generate endothelial dysfunction, but it is unclear whether the impairment of function of brain endothelial cells at the blood-brain barrier is secondary to plasma-derived or placental-derived exosomes. In this study, we evaluated the effect of small extracellular vesicles isolated from plasma samples of women with preeclampsia (n=12) and women with normal pregnancy (n=11) as well as from human placental explants from normotensive pregnancies (n=6) subjected to hypoxia (1% oxygen) on the integrity of the blood-brain barrier, using both in vitro and animal models. Exposure of human-derived brain endothelial cell monolayers to plasma and plasma-derived small extracellular vesicles from preeclamptic pregnancies increased the permeability and reduced the transendothelial electrical resistance. A similar outcome was observed with hypoxic placental-derived small extracellular vesicles, which also increased the permeability to Evan’s blue in the brain of C57BL6 nonpregnant mice. Cotreatment with magnesium sulfate reversed the effects elicited by plasma, plasma-derived, and hypoxic placental-derived small extracellular vesicles in the employed models. Thus, circulating small extracellular vesicles in plasma from women with preeclampsia or from hypoxic placentae disrupt the blood-brain barrier, which can be prevented using magnesium sulfate. These findings provide new insights into the pathophysiology of cerebral complications associated with preeclampsia.

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