scholarly journals The anti-cancer potency of photodynamic therapy of a novel chlorin derivative Amidochlorin p6 (ACP)

RSC Advances ◽  
2017 ◽  
Vol 7 (65) ◽  
pp. 40873-40880 ◽  
Author(s):  
Hongyue Zhang ◽  
Wenting Li ◽  
Guanghui Tan ◽  
Guohua Ding ◽  
Zhiqiang Wang ◽  
...  
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Cell Viability ◽  
Hela Cells ◽  
Anti Cancer ◽  
Chlorin Derivative

Amidochlorin p6 (ACP) was uptaken by HeLa cells, showing excellent phototoxicity (the cell viability was 21% at a concentration of 8 μmol L−1), resulting in cell death.

scholarly journals Anticancer Effects of Propionic Acid Inducing Cell Death in Cervical Cancer Cells

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4951
Author(s):  
Chau-Ha Pham ◽  
Joo-Eun Lee ◽  
Jinha Yu ◽  
Sung-Hoon Lee ◽  
Kyung-Rok Yu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Death ◽  
Hela Cell ◽  
Cell Viability ◽  
Propionic Acid ◽  
Hela Cells ◽  
Cancer Therapeutics ◽  
Short Chain Fatty Acids ◽  
Bacterial Fermentation ◽  
Protein Levels

Recent studies found that short-chain fatty acids (SCFAs), which are produced through bacterial fermentation in the gastrointestinal tract, have oncoprotective effects against cervical cancer. The most common SCFAs that are well known include acetic acid, butyric acid, and propionic acid, among which propionic acid (PA) has been reported to induce apoptosis in HeLa cells. However, the mechanism in which SCFAs suppress HeLa cell viability remain poorly understood. Our study aims to provide a more detailed look into the mechanism of PA in HeLa cells. Flow cytometry analysis revealed that PA induces reactive oxygen species (ROS), leading to the dysfunction of the mitochondrial membrane. Moreover, PA inhibits NF-κB and AKT/mTOR signaling pathways and induces LC3B protein levels, resulting in autophagy. PA also increased the sub-G1 cell population that is characteristic of cell death. Therefore, the results of this study propose that PA inhibits HeLa cell viability through a mechanism mediated by the induction of autophagy. The study also suggests a new approach for cervical cancer therapeutics.

scholarly journals An Assessment of InP/ZnS as Potential Anti-Cancer Therapy: Quantum Dot Treatment Increases Apoptosis in HeLa Cells

2021 ◽  
Vol 2 (1) ◽  
pp. 16-32
Author(s):  
Victoria Davenport ◽  
Cullen Horstmann ◽  
Rishi Patel ◽  
Qihua Wu ◽  
Kyoungtae Kim
Keyword(s):  
Cell Viability ◽  
Hela Cells ◽  
Fluorescent Imaging ◽  
Oxygen Species ◽  
Transcriptome Profile ◽  
Xtt Assay ◽  
Anti Cancer ◽  
Cervical Cancer Cells ◽  
Nitrogen Radicals ◽  
Ic50 Concentration

InP/ZnS quantum dots (QDs) are an emerging option in QD technologies for uses of fluorescent imaging as well as targeted drug and anticancer therapies based on their customizable properties. In this study we explored effects of InP/ZnS when treated with HeLa cervical cancer cells. We employed XTT viability assays, reactive oxygen species (ROS) analysis, and apoptosis analysis to better understand cytotoxicity extents at different concentrations of InP/ZnS. In addition, we compared the transcriptome profile from the QD-treated HeLa cells with that of untreated HeLa cells to identify changes to the transcriptome in response to the QD. RT-qPCR assay was performed to confirm the findings of transcriptome analysis, and the QD mode of action was illustrated. Our study determined both IC50 concentration of 69 µg/mL and MIC concentration of 167 µg/mL of InP/ZnS. It was observed via XTT assay that cell viability was decreased significantly at the MIC. Production of superoxide, measured by ROS assay with flow cytometry, was decreased, whereas levels of nitrogen radicals increased. Using analysis of apoptosis, we found that induced cell death in the QD-treated samples was shown to be significantly increased when compared to untreated cells. We conclude InP/ZnS QD to decrease cell viability by inducing stress via ROS levels, apoptosis induction, and alteration of transcriptome.

scholarly journals Role of DNA Damage and MMP Loss in Radiosensitization-Induced Apoptosis by Ellagic Acid in HeLa Cells

2020 ◽  
Vol 25775790 ◽  
pp. 1-1
Author(s):  
Vidhula R Ahire ◽  
◽  
Amit Kumar ◽  
Sushma Bhosle ◽  
Kaushala Prasad Mishra ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Cell Survival ◽  
Hela Cells ◽  
Ellagic Acid ◽  
Mitochondrial Membrane ◽  
Radiation Treatment ◽  
Γ Radiation ◽  
Anti Cancer

Ellagic acid (EA) is a polyphenol found in grapes, pomegranates, walnuts, etc. exhibits anti-cancer properties. The current study was conducted to understand the radiosensitizing role of EA on HeLa cells. Monotherapy of EA and radiation was initially studied on HeLa cells. The addition of EA before the radiation treatment subsequently made DNA more susceptible to damage thereby developing DNA beaks, which are known to be lethal for cell survival. This was evaluated by performing comet and γ-foci formation assay. Other assays which included cell-cycle distribution, clonogenic cell survival assay, mitochondrial membrane drop, and apoptosis were performed to evaluate the effect of EA with radiation. Our results demonstrate that, when cells were exposed to the combinatorial treatment of EA (10µM) and 2Gy of γ-radiation there was augmented cell death, lesser cell-proliferation, reduction in the colony-forming ability, increased DNA tail length, more number of γ-foci persisting even after 24h, enhanced apoptosis, augmented drop in the mitochondrial membrane potential and a G1 cell-cycle arrest. These results suggest that EA exhibits not only anti-cancer properties in terms of cell-death but also exhibits a radiosensitizing effect when given in combination with γ-radiation. Thus, it can be concluded that EA not only exhibits anticancer effects but also has potential in radiosensitizing HeLa cells.

Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs

Biomaterials ◽  
2011 ◽  
Vol 32 (14) ◽  
pp. 3637-3646 ◽  
Author(s):  
Yan-Qing Guan ◽  
Zhibin Li ◽  
Jiamei Chen ◽  
Huimin Tao ◽  
Wenwen Wang ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Hela Cells ◽  
Cancer Drugs ◽  
Anti Cancer

scholarly journals Inhibition Capacity of the n-Hexane Fraction of Myrmecodia pendens as a Potential Anti-Cancer in Breast and Cervical Cancer: In Vitro Study

2020 ◽  
Vol 11 (3) ◽  
pp. 115
Author(s):  
Muhammad Hasan Bashari ◽  
Eveline Yuniarti ◽  
Tenny Putri ◽  
Nurul Qomarilla ◽  
Dikdik Kurnia ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cervical Cancer ◽  
Cell Death ◽  
Cell Migration ◽  
Mtt Assay ◽  
Hela Cells ◽  
Hexane Fraction ◽  
Scratch Assay ◽  
Anti Cancer ◽  
Mcf 7

Breast cancer (BC) and cervical cancer (CC) have a high prevalence and mortality rate worldwide. Despite the availability of advanced treatment, resistance to conventional chemotherapies has emerged. Myrmecodia pendens, one of the species of Sarang Semut (local name), possess a potential of antitumor effects by inducing cell death different cancer cell entities. This study aimed to assess anti-tumor activities of n-hexane fraction of M. pendens in inhibiting cell survival and cell migration in BC and CC cells. M. pendens was extracted in methanol then fractionated using n-hexane or ethyl acetate. BC cells including MCF-7 (luminal A), HCC-1954 (HER2+) cells and CC Hela cells were treated with M. pendens extracts to evaluate cytotoxic activity using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay as well as anti-cell migration using scratch assay. We also analyzed inhibitory concentration 50 (IC50) of n-hexane fraction in BC and CC cells. We started with comparing cytotoxicity activities of methanol extract, ethyl acetate and n-hexane fractions of M. pendens. Data showed that the n-hexane fraction was the most potent inducing BC cell death. Therefore, we used the n-hexane fraction for further experiments. Interestingly, IC50 of this fraction in HCC-1954 and Hela cells were lower than in MCF-7 cells, 16; 13 and 60 ppm, respectively. Moreover, the low concentrations of n-hexane fraction inhibited HeLa cells migration, compared to control group (p<0.05). The n-hexane fraction of M. pendens shows promising anti-cancer agent, by inhibiting BC and CC cell survival as well as inhibiting CC cells migration.Keywords: breast cancer, cervical cancer, MTT assay, Sarang Semut, scratch assay

Cell death pathway investigations on Gd2O3:Yb,Tm-MC540 mediated photodynamic therapy of HeLa cells

2018 ◽  
Vol 14 (5) ◽  
pp. 1743-1744
Author(s):  
Yajun Sun ◽  
Weihua Wang ◽  
Weikang Wang ◽  
Nailin Yang ◽  
Wei Wang ◽  
...  
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Hela Cells ◽  
Cell Death Pathway

ER stress, autophagy and immunogenic cell death in photodynamic therapy-induced anti-cancer immune responses

2014 ◽  
Vol 13 (3) ◽  
pp. 474-487 ◽  
Author(s):  
Abhishek D. Garg ◽  
Patrizia Agostinis
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Immune System ◽  
Immune Responses ◽  
Er Stress ◽  
Cancer Cell ◽  
Immunogenic Cell Death ◽  
Danger Signals ◽  
Anti Cancer

Few, selected anticancer therapeutic regimens like Hypericin-PDT are able to induce a promising kind of cancer cell demise called immunogenic cell death (ICD), which can activate the immune system owing to the spatiotemporally defined emission of danger signals.

Fluorous photosensitizers enhance photodynamic therapy with perfluorocarbon nanoemulsions

2017 ◽  
Author(s):  
Ellen Sletten ◽  
Rachael A. Day ◽  
Daniel A. Estabrook ◽  
Jessica K. Logan
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Induce Cell Death ◽  
Perfluorocarbon Nanoemulsions

<p>Photodynamic therapy (PDT) requires photosensitizer, light, and oxygen to induce cell death. The majority of efforts to advance PDT focus only on the first two components. Here, we employ perfluorocarbon nanoemulsions to simultaneously deliver oxygen and photosensitizer. We find that the implementation of fluorous soluble photosensitizers enhances the efficacy of PDT. </p>

Stew in its Own Juice: Protein Homeostasis Machinery Inhibition Reduces Cell Viability in Multiple Myeloma Cell Lines

2019 ◽  
Vol 19 (2) ◽  
pp. 112-119 ◽  
Author(s):  
Mariana B. de Oliveira ◽  
Luiz F.G. Sanson ◽  
Angela I.P. Eugenio ◽  
Rebecca S.S. Barbosa-Dantas ◽  
Gisele W.B. Colleoni
Keyword(s):  
Multiple Myeloma ◽  
Cell Death ◽  
Cell Viability ◽  
Cell Lines ◽  
Treatment Options ◽  
Compensatory Mechanism ◽  
Protein Homeostasis ◽  
Protein Response ◽  
Rpmi 8226

Introduction:Multiple myeloma (MM) cells accumulate in the bone marrow and produce enormous quantities of immunoglobulins, causing endoplasmatic reticulum stress and activation of protein handling machinery, such as heat shock protein response, autophagy and unfolded protein response (UPR).Methods:We evaluated cell lines viability after treatment with bortezomib (B) in combination with HSP70 (VER-15508) and autophagy (SBI-0206965) or UPR (STF- 083010) inhibitors.Results:For RPMI-8226, after 72 hours of treatment with B+VER+STF or B+VER+SBI, we observed 15% of viable cells, but treatment with B alone was better (90% of cell death). For U266, treatment with B+VER+STF or with B+VER+SBI for 72 hours resulted in 20% of cell viability and both treatments were better than treatment with B alone (40% of cell death). After both triplet combinations, RPMI-8226 and U266 presented the overexpression of XBP-1 UPR protein, suggesting that it is acting as a compensatory mechanism, in an attempt of the cell to handle the otherwise lethal large amount of immunoglobulin overload.Conclusion:Our in vitro results provide additional evidence that combinations of protein homeostasis inhibitors might be explored as treatment options for MM.

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