scholarly journals Effect of composition and macropore percentage on mechanical and in vitro cell proliferation and differentiation properties of 3D printed HA/β-TCP scaffolds

RSC Advances ◽  
2017 ◽  
Vol 7 (68) ◽  
pp. 43186-43196 ◽  
Author(s):  
Ningbo Zhao ◽  
Yanen Wang ◽  
Lei Qin ◽  
Zhengze Guo ◽  
Dehua Li
Keyword(s):  
Cell Proliferation ◽  
3D Printing ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
3D Printed

HA/β-TCP scaffolds were fabricated by 3D printing and exhibited desirable biocompatibilityin vitro.

scholarly journals 3234 Cell Proliferation and Differentiation in 3D printed Polycarbonate Urethane Porous Scaffolds

2019 ◽  
Vol 3 (s1) ◽  
pp. 141-141
Author(s):  
Bijan Abar ◽  
Alejandro Aalleja ◽  
Cambre Kelly ◽  
Natalia Von Windheim ◽  
Jennifer West ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cellular Proliferation ◽  
Porous Scaffold ◽  
Biological Response ◽  
Collagen Gel ◽  
Porous Scaffolds ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Study Population ◽  
3D Printed

OBJECTIVES/SPECIFIC AIMS: The aim of this study is to understand how porosity and collagen filling impact cell proliferation and differentiation in 3D printed scaffolds. METHODS/STUDY POPULATION: 3 groups of scaffolds will be 3D printed using FDM: solid scaffold, porous scaffold and porous scaffold with collagen gel (n=10 for each group) Internal geometries and surface structure will be analyzed using micro CT and Scanning Electron Mi RESULTS/ANTICIPATED RESULTS: We hypothesize that porosity and collagen filler will increase signal from Picogreen assay and ALP assay when normalized to scaffold surface area, indicating enhanced cell proliferation and differentiation. DISCUSSION/SIGNIFICANCE OF IMPACT: 3D printing PCU is a relatively new technique with very little published in the literature. Previous work has focused on the mechanical properties and not the biological response to the polymer. Understanding how to optimize cellular proliferation and differentiation can lead to the development of better implants that will integrate into the host’s structure and facilitate tissue regeneration.

scholarly journals Improved Muscle Function After Injury with the Application of a Biological Decellularized Matrix

2020 ◽  
Author(s):  
Amina El Ayadi ◽  
Melody R.S. Threlkeld ◽  
Steven E. Wolf ◽  
Juquan Song
Keyword(s):  
Cell Proliferation ◽  
Muscle Cell ◽  
Muscle Function ◽  
Potential Benefit ◽  
Time Course ◽  
Isometric Force ◽  
Cell Proliferation And Differentiation ◽  
Function Recovery ◽  
Proliferation And Differentiation

Abstract Background: Skeletal muscle injury leads to loss of muscle function that lasts well into recovery and can be permanent. Application of the novel bio-scaffold termed porcine-derived urinary bladder matrix (UBM) has a potential benefit to mitigate injury through tissue regeneration. To date, findings of potential benefit in animal models were limited to short assessment times. The purpose of this study was to investigate whether UBM treatment 14 days after injury sustainably improves the recovery of muscle function in injured mice. Methods: C57BL/6 adult male mice received bilateral laceration injuries on the gastrocnemius (GN) muscle under anesthesia and were then treated with vehicle or 150 µg of UBM nanoparticles. Treatment was applied immediately after injury or 14 days later. Muscle isometric force was measured 60 days after injury. Previous time course analyses have shown that muscle function did not start to improve until after 42 days after injury. Therefore, we designed a second experiment to trace the time course of UBM effects on muscle function recovery by measuring the isometric muscle force at 49 and 90 days after injury. In vitro, we analyzed the effects of UBM on muscle cell proliferation and differentiation. Results: UBM promotes muscle cell proliferation and differentiation. Twitch (Pt), tetanic (Po) force and maximal fatigue were significantly decreased in the injured mice on day 60. Muscle fatigue maximum force significantly recovered when UBM treatment was applied 14 days after injury (p<0.05) but not when UBM was applied immediately after the injury. Time course analysis demonstrated that UBM improvement of Pt and Po was evident by day 49 after injury (p<0.05). However, no further muscle function improvement was observed on day 90. Conclusions: Delayed treatment with the UBM improves muscle function recovery following laceration injury starting 49 days after injury. These effects may be mediated by improvements in muscle cell proliferation and differentiation. This animal model is suitable to test other therapeutic strategies to improve muscle function after injury.

A review on 3D printing in tissue engineering applications

2022 ◽  
Vol 0 (0) ◽  
Author(s):  
Mohan Prasath Mani ◽  
Madeeha Sadia ◽  
Saravana Kumar Jaganathan ◽  
Ahmad Zahran Khudzari ◽  
Eko Supriyanto ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Cell Adherence ◽  
Engineering Applications ◽  
Proliferation And Differentiation ◽  
3D Printed ◽  
Printing Techniques

Abstract In tissue engineering, 3D printing is an important tool that uses biocompatible materials, cells, and supporting components to fabricate complex 3D printed constructs. This review focuses on the cytocompatibility characteristics of 3D printed constructs, made from different synthetic and natural materials. From the overview of this article, inkjet and extrusion-based 3D printing are widely used methods for fabricating 3D printed scaffolds for tissue engineering. This review highlights that scaffold prepared by both inkjet and extrusion-based 3D printing techniques showed significant impact on cell adherence, proliferation, and differentiation as evidenced by in vitro and in vivo studies. 3D printed constructs with growth factors (FGF-2, TGF-β1, or FGF-2/TGF-β1) enhance extracellular matrix (ECM), collagen I content, and high glycosaminoglycan (GAG) content for cell growth and bone formation. Similarly, the utilization of 3D printing in other tissue engineering applications cannot be belittled. In conclusion, it would be interesting to combine different 3D printing techniques to fabricate future 3D printed constructs for several tissue engineering applications.

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