scholarly journals Comparative bone regeneration study of hardystonite and hydroxyapatite as filler in critical-sized defect of rat calvaria

RSC Advances ◽  
2017 ◽  
Vol 7 (60) ◽  
pp. 37522-37533 ◽  
Author(s):  
Kunal Khanna ◽  
Amit Jaiswal ◽  
Rohit V. Dhumal ◽  
Nilakash Selkar ◽  
Pradip Chaudhari ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Healing ◽  
Bone Grafts ◽  
Skull Defect ◽  
Defect Model ◽  
Rat Calvaria ◽  
Ceramic Hydroxyapatite ◽  
Regeneration Study ◽  
Critical Sized Defect

Bone grafts made from nanofibrous polycaprolactone loaded with bone-mimicking ceramic hydroxyapatite or hardystonite showed efficient bone healing in an in vivo rat skull defect model.

scholarly journals Plain and Stromal Cells Seeded Collagen Nanofibers Promote Bone Regeneration In vivo. A Rat Calvaria Defect Model

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Bou-Akl Therese ◽  
Wu Bin ◽  
Daly-Seiler Conor ◽  
Dietz Paula ◽  
Ren Weiping ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Stromal Cells ◽  
Defect Model ◽  
Rat Calvaria

scholarly journals Canine Mesenchymal Stromal Cell-Mediated Bone Regeneration is Enhanced in the Presence of Sub-Therapeutic Concentrations of BMP-2 in a Murine Calvarial Defect Model

2021 ◽  
Vol 9 ◽  
Author(s):  
Lauren K. Dobson ◽  
Suzanne Zeitouni ◽  
Eoin P. McNeill ◽  
Robert N. Bearden ◽  
Carl A. Gregory ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Healing ◽  
Human Mscs ◽  
Calvarial Defect ◽  
Defect Model ◽  
Alp Activity ◽  
Calvarial Defects ◽  
Species Specific

Novel bone regeneration strategies often show promise in rodent models yet are unable to successfully translate to clinical therapy. Sheep, goats, and dogs are used as translational models in preparation for human clinical trials. While human MSCs (hMSCs) undergo osteogenesis in response to well-defined protocols, canine MSCs (cMSCs) are more incompletely characterized. Prior work suggests that cMSCs require additional agonists such as IGF-1, NELL-1, or BMP-2 to undergo robust osteogenic differentiation in vitro. When compared directly to hMSCs, cMSCs perform poorly in vivo. Thus, from both mechanistic and clinical perspectives, cMSC and hMSC-mediated bone regeneration may differ. The objectives of this study were twofold. The first was to determine if previous in vitro findings regarding cMSC osteogenesis were substantiated in vivo using an established murine calvarial defect model. The second was to assess in vitro ALP activity and endogenous BMP-2 gene expression in both canine and human MSCs. Calvarial defects (4 mm) were treated with cMSCs, sub-therapeutic BMP-2, or the combination of cMSCs and sub-therapeutic BMP-2. At 28 days, while there was increased healing in defects treated with cMSCs, defects treated with cMSCs and BMP-2 exhibited the greatest degree of bone healing as determined by quantitative μCT and histology. Using species-specific qPCR, cMSCs were not detected in relevant numbers 10 days after implantation, suggesting that bone healing was mediated by anabolic cMSC or ECM-driven cues and not via engraftment of cMSCs. In support of this finding, defects treated with cMSC + BMP-2 exhibited robust deposition of Collagens I, III, and VI using immunofluorescence. Importantly, cMSCs exhibited minimal ALP activity unless cultured in the presence of BMP-2 and did not express endogenous canine BMP-2 under any condition. In contrast, human MSCs exhibited robust ALP activity in all conditions and expressed human BMP-2 when cultured in control and osteoinduction media. This is the first in vivo study in support of previous in vitro findings regarding cMSC osteogenesis, namely that cMSCs require additional agonists to initiate robust osteogenesis. These findings are highly relevant to translational cell-based bone healing studies and represent an important finding for the field of canine MSC-mediated bone regeneration.

scholarly journals Zn-Containing Membranes for Guided Bone Regeneration in Dentistry

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1797
Author(s):  
Manuel Toledano ◽  
Marta Vallecillo-Rivas ◽  
María T. Osorio ◽  
Esther Muñoz-Soto ◽  
Manuel Toledano-Osorio ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bone Regeneration ◽  
Bone Healing ◽  
Bone Repair ◽  
Complex Modulus ◽  
Bacterial Colonization ◽  
Guided Bone Regeneration ◽  
M2 Macrophage

Barrier membranes are employed in guided bone regeneration (GBR) to facilitate bone in-growth. A bioactive and biomimetic Zn-doped membrane with the ability to participate in bone healing and regeneration is necessary. The aim of the present study is to state the effect of doping the membranes for GBR with zinc compounds in the improvement of bone regeneration. A literature search was conducted using electronic databases, such as PubMed, MEDLINE, DIMDI, Embase, Scopus and Web of Science. A narrative exploratory review was undertaken, focusing on the antibacterial effects, physicochemical and biological properties of Zn-loaded membranes. Bioactivity, bone formation and cytotoxicity were analyzed. Microstructure and mechanical properties of these membranes were also determined. Zn-doped membranes have inhibited in vivo and in vitro bacterial colonization. Zn-alloy and Zn-doped membranes attained good biocompatibility and were found to be non-toxic to cells. The Zn-doped matrices showed feasible mechanical properties, such as flexibility, strength, complex modulus and tan delta. Zn incorporation in polymeric membranes provided the highest regenerative efficiency for bone healing in experimental animals, potentiating osteogenesis, angiogenesis, biological activity and a balanced remodeling. Zn-loaded membranes doped with SiO2 nanoparticles have performed as bioactive modulators provoking an M2 macrophage increase and are a potential biomaterial for promoting bone repair. Zn-doped membranes have promoted pro-healing phenotypes.

scholarly journals Bone Regeneration Assessment of Polycaprolactone Membrane on Critical-Size Defects in Rat Calvaria

Membranes ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 124
Author(s):  
Ana Paula Farnezi Bassi ◽  
Vinícius Ferreira Bizelli ◽  
Tamires Mello Francatti ◽  
Ana Carulina Rezende de Moares Ferreira ◽  
Járede Carvalho Pereira ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Healing ◽  
Inflammatory Responses ◽  
Blood Clot ◽  
Healing Process ◽  
Male Rats ◽  
Control Group ◽  
Collagen Membrane ◽  
Large Area ◽  
Rat Calvaria

Biomaterials for use in guided bone regeneration (GBR) are constantly being investigated and developed to improve clinical outcomes. The present study aimed to comparatively evaluate the biological performance of different membranes during the bone healing process of 8 mm critical defects in rat calvaria in order to assess their influence on the quality of the newly formed bone. Seventy-two adult male rats were divided into three experimental groups (n = 24) based on the membranes used: the CG—membrane-free control group (only blood clot, negative control), BG—porcine collagen membrane group (Bio-Guide®, positive control), and the PCL—polycaprolactone (enriched with 5% hydroxyapatite) membrane group (experimental group). Histological and histometric analyses were performed at 7, 15, 30, and 60 days postoperatively. The quantitative data were analyzed by two-way ANOVA and Tukey’s test (p < 0.05). At 7 and 15 days, the inflammatory responses in the BG and PCL groups were significantly different (p < 0.05). The PCL group, at 15 days, showed a large area of newly formed bone. At 30 and 60 days postoperatively, the PCL and BG groups exhibited similar bone healing, including some specimens showing complete closure of the critical defect (p = 0.799). Thus, the PCL membrane was biocompatible, and has the potential to help with GBR procedures.

Melatonin decorated 3D-printed beta-tricalcium phosphate scaffolds promoting bone regeneration in a rat calvarial defect model

2019 ◽  
Vol 7 (20) ◽  
pp. 3250-3259 ◽  
Author(s):  
Yali Miao ◽  
Yunhua Chen ◽  
Xiao Liu ◽  
Jingjing Diao ◽  
Naru Zhao ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Calvarial Defect ◽  
Defect Model ◽  
Beta Tricalcium Phosphate ◽  
3D Printed ◽  
Rat Calvarial Defect

3D-printed β-TCP scaffolds decorated with melatonin via dopamine mussel-inspired chemistry enhance the osteogenesis and in vivo bone regeneration.

scholarly journals Effect of herbal extracts on bone regeneration in a rat calvaria defect model and screening system

2018 ◽  
Vol 44 (2) ◽  
pp. 79 ◽  
Author(s):  
Dong-Hwan Lee ◽  
Il-Kyu Kim ◽  
Hyun-Young Cho ◽  
Ji-Hoon Seo ◽  
Jun-Min Jang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Screening System ◽  
Herbal Extracts ◽  
Defect Model ◽  
Rat Calvaria

Ultraviolet-Crosslinkable and Injectable Chitosan/Hydroxyapatite Hybrid Hydrogel for Critical Size Calvarial Defect Repair In Vivo

2015 ◽  
Vol 6 (4) ◽  
Author(s):  
Baoqiang Li ◽  
Lei Wang ◽  
Yu Hao ◽  
Daqing Wei ◽  
Ying Li ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Critical Size ◽  
Rabbit Model ◽  
Single Step ◽  
Calvarial Defect ◽  
Defect Model ◽  
Calvarial Bone ◽  
Hybrid Hydrogel ◽  
Defect Site

To promote bone regeneration in vivo using critical-size calvarial defect model, hybrid hydrogel was prepared by mixing chitosan with hydroxyapatite (HA) and ultraviolet (UV) irradiation in situ. The hydrosoluble, UV-crosslinkable and injectable N-methacryloyl chitosan (N-MAC) was synthesized via single-step N-acylation reaction. The chemical structure was confirmed by 1H-NMR and FTIR spectroscopy. N-MAC hydrogel presented a microporous structure with pore sizes ranging from 10 to 60 μm. Approximately 80% cell viability of N-MAC hydrogel against encapsulated 3T3 cell indicated that N-MAC is an emerging candidate for mimicking native extracellular matrix (ECM). N-MAC hydrogel hybridized with HA was used to accelerate regeneration of calvarial bone using rabbit model. The effects of hybrid hydrogels to promote bone regeneration were evaluated using critical size calvarial bone defect model. The healing effects of injectable hydrogels with/without HA for bone regeneration were investigated by analyzing X-ray image after 4 or 6 weeks. The results showed that the regenerated new bone for N-MAC 100 was significantly greater than N-MAC without HA and untreated controls. The higher HA content in N-MAC/HA hybrid hydrogel benefited the acceleration of bone regeneration. About 50% closure of defect site after 6 weeks postimplantation demonstrated potent osteoinductivity of N-MAC 100 UV-crosslinkable and injectable N-MAC/HA hybrid hydrogel would allow serving as a promising biomaterial for bone regeneration using the critical-size calvarial defect.

scholarly journals The Influence of Eggshell on Bone Regeneration in Preclinical In Vivo Studies

Biology ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 476
Author(s):  
Horia Opris ◽  
Cristian Dinu ◽  
Mihaela Baciut ◽  
Grigore Baciut ◽  
Ileana Mitre ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Animal Studies ◽  
Meta Analysis ◽  
Bone Matrix ◽  
In Vivo Studies ◽  
Defect Model ◽  
Exclusion Criteria ◽  
Freeze Dried ◽  
Grafting Materials

The aim of this study is to systemically review the available evidence on the in vivo behavior of eggshell as a guided bone regeneration substitute material. Five databases (PubMed, Cochrane, Web of Science, Scopus, EMBASE) were searched up to October 2020. In vivo animal studies with a bone defect model using eggshell as a grafting material were included. Risk of bias was assessed using SYRCLE tool and the quality assessment using the ARRIVE guidelines. Overall, a total of 581 studies were included in the study, 187 after duplicate removal. Using the inclusion and exclusion criteria 167 records were further excluded. The full text of the remaining 20 articles was assessed for eligibility and included in the qualitative and quantitative assessment synthesis. There were different methods of obtaining eggshell grafting materials. Eggshell is a biocompatible grafting material, with osteoconduction proprieties. It forms new bone similar to Bio-Oss and demineralized freeze-dried bone matrix. It can be combined with other materials to enhance its proprieties. Due to the high variability of the procedures, animals, production and assessment methods, no meta-analysis could be performed. Eggshell might be considered a promising biomaterial to be used in bone grafting procedures, though further research is needed.

scholarly journals Effect of Multilaminate Small Intestinal Submucosa as a Barrier Membrane on Bone Formation in a Rabbit Mandible Defect Model

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Weiyi Wu ◽  
Bowen Li ◽  
Yuhua Liu ◽  
Xinzhi Wang ◽  
Lin Tang
Keyword(s):  
Bone Regeneration ◽  
Porous Scaffold ◽  
Small Intestinal Submucosa ◽  
Control Group ◽  
Collagen Membrane ◽  
Defect Model ◽  
Barrier Membrane ◽  
Small Intestinal ◽  
Intestinal Submucosa

A barrier membrane (BM) is essential for guided bone regeneration (GBR) procedures. Absorbable BMs based on collagen have been widely applied clinically due to their excellent biocompatibility. The extracellular matrix (ECM) provides certain advantages that can compensate for the rapid degradation and insufficient mechanical strength of pure collagen membrane due to the porous scaffold structure. Recently, small intestinal submucosa (SIS), one of the most widely used ECM materials, has drawn much attention in bone tissue engineering. In this study, we adopted multilaminate SIS (mSIS) as a BM and evaluated its in vivo and in vitro properties. mSIS exhibited a multilaminate structure with a smooth upper surface and a significantly coarser bottom layer according to microscopic observation. Tensile strength was 13.10 ± 2.56 MPa. In in vivo experiments, we selected a rabbit mandibular defect model and subcutaneous implantation to compare osteogenesis and biodegradation properties with one of the most commonly used commercial collagen membranes. mSIS was retained for up to 3 months and demonstrated longer biodegradation time than commercial collagen membrane. Quantification of bone regeneration revealed significant differences in each group. Micro-computed tomography (micro-CT) revealed that the quantity and maturity of bones in the mSIS group were significantly higher than those in the blank control group (P < 0.05) and were similar to those in a commercial collagen membrane group (P > 0.05) at 4 and 12 weeks after surgery. Hematoxylin and eosin staining revealed large amounts of mature lamellar bone at 12 weeks in mSIS and commercial collagen membrane groups. Therefore, we conclude that mSIS has potential as a future biocompatible BM in GBR procedures.

scholarly journals Longitudinal in Vivo Analysis of the Region-Specific Efficacy of Parathyroid Hormone in a Rat Cortical Defect Model

Endocrinology ◽  
2008 ◽  
Vol 150 (4) ◽  
pp. 1570-1579 ◽  
Author(s):  
David E. Komatsu ◽  
Kellie A. Brune ◽  
Hong Liu ◽  
Allen L. Schmidt ◽  
Bomie Han ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Bone Healing ◽  
Quantitative Computed Tomography ◽  
Type I ◽  
Defect Model ◽  
Procollagen Type ◽  
Cortical Defect ◽  
Defect Sites ◽  
Procollagen Type I

PTH has been shown to enhance fracture repair; however, exactly when and where PTH acts in this process remains to be elucidated. Therefore, we conducted a longitudinal, region-specific analysis of bone regeneration in mature, osteopenic rats using a cortical defect model. Six-month-old rats were ovariectomized, and allowed to lose bone for 2 months, before being subjected to bilateral 2-mm circular defects in their femoral diaphyses. They were then treated for 5 wk with hPTH1–38 at doses of 0, 3, 10, or 30 μg/kg · d and scanned weekly by in vivo quantitative computed tomography. Quantitative computed tomography analyses showed temporal, dose-dependent increases in mineralization in the defects, intramedullary (IM) spaces, and whole diaphyses at the defect sites. Histomorphometry confirmed PTH stimulation of primarily woven bone in the defects and IM spaces, but not the periosteum. After necropsy, biomechanical testing identified an increase in strength at the highest PTH dose. Serum procollagen type I N-terminal propeptide concentration showed a transient increase due to drilling, but procollagen type I N-terminal propeptide also increased with PTH treatment, whereas tartrate-resistant acid phosphatase unexpectedly decreased. Analyses of lumber vertebra confirmed systemic efficacy of PTH at a nonfracture site. In summary, PTH dose dependently induced new bone formation within defects, at endocortical surfaces, and in IM spaces, resulting in faster and greater bone healing, as well as efficacy at other skeletal sites. The effects of PTH were kinetic, region specific, and most apparent at high doses that may not be entirely clinically relevant; therefore, clinical studies are necessary to clarify the therapeutic utility of PTH in bone healing.

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