scholarly journals Isolation of label-free and viable circulating tumour cells (CTCs) from blood samples of cancer patients through a two-step process: negative selection-type immunomagnetic beads and spheroid cell culture-based cell isolation

RSC Advances ◽  
2017 ◽  
Vol 7 (47) ◽  
pp. 29339-29349 ◽  
Author(s):  
Chia-Jung Liao ◽  
Chia-Hsun Hsieh ◽  
Hung-Ming Wang ◽  
Wen-Pin Chou ◽  
Tzu-Keng Chiu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cancer Patients ◽  
Negative Selection ◽  
Circulating Tumour Cells ◽  
Label Free ◽  
Immunomagnetic Beads ◽  
Purification Method ◽  
Blood Samples ◽  
Step Process ◽  
Spheroid Cell Culture

A two-step CTCs purification method to isolate viable, label-free, all possible, and purity improved CTCs.

Simultaneous biochemical and functional phenotyping of single circulating tumor cells using ultrahigh throughput and recovery microfluidic devices

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yang Liu ◽  
Wujun Zhao ◽  
Rui Cheng ◽  
Jamie Hodgson ◽  
Mary Egan ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Devices ◽  
Tumour Cells ◽  
Circulating Tumour Cells ◽  
Dynamic Nature ◽  
Blood Samples

Profiling circulating tumour cells (CTCs) in cancer patients’ blood samples is critical to understanding the complex and dynamic nature of metastasis. This task is challenged by the fact that CTCs...

scholarly journals The Integration of a Three-Dimensional Spheroid Cell Culture Operation in a Circulating Tumor Cell (CTC) Isolation and Purification Process: A Preliminary Study of the Clinical Significance and Prognostic Role of the CTCs Isolated from the Blood Samples of Head and Neck Cancer Patients

Cancers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 783 ◽  
Author(s):  
Chia-Jung Liao ◽  
Chia-Hsun Hsieh ◽  
Feng-Chun Hung ◽  
Hung-Ming Wang ◽  
Wen-Pin Chou ◽  
...  
Keyword(s):  
Cell Culture ◽  
Tumor Cell ◽  
Head And Neck ◽  
Negative Selection ◽  
Cancer Metastasis ◽  
Circulating Tumor Cell ◽  
Isolation Method ◽  
Isolation And Purification ◽  
Mesenchymal Transition ◽  
Spheroid Cell Culture

Conventional positive and negative selection-based circulating tumor cell (CTC) isolation methods might generally ignore metastasis-relevant CTCs that underwent epithelial-to- mesenchymal transition and suffer from a low CTC purity problem, respectively. To address these issues, we previously proposed a 2-step CTC isolation method integrating a negative selection CTC isolation and subsequent spheroid cell culture. In addition to its ability to isolate CTCs, more importantly, the spheroid cell culture used could serve as a cell culture model mimicking the process of new tumor tissue formation during cancer metastasis. Therefore, it is promising not only to selectively isolate metastasis-relevant CTCs but also to test the potential of cancer metastasis and thus the prognosis of disease. To explore these issues, experiments were performed. The key findings of this study demonstrated that the method was able to harvest both epithelial (E)- and mesenchymal (M)-type CTCs without selection bias. Moreover, both the M-type CTC count and the information obtained from the multidrug resistance-associated protein 2 (MRP2) and MRP5 gene expression analysis of the CTCs isolated via the 2-step CTC isolation method might be able to serve as prognostic factors for progression-free survival in head and neck squamous cell carcinoma.

scholarly journals Acoustic separation of circulating tumor cells

2015 ◽  
Vol 112 (16) ◽  
pp. 4970-4975 ◽  
Author(s):  
Peng Li ◽  
Zhangming Mao ◽  
Zhangli Peng ◽  
Lanlan Zhou ◽  
Yuchao Chen ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Peripheral Blood ◽  
Cancer Biology ◽  
Cancer Metastasis ◽  
Surface Acoustic Waves ◽  
Clinical Samples ◽  
Label Free ◽  
Blood Samples

Circulating tumor cells (CTCs) are important targets for cancer biology studies. To further elucidate the role of CTCs in cancer metastasis and prognosis, effective methods for isolating extremely rare tumor cells from peripheral blood must be developed. Acoustic-based methods, which are known to preserve the integrity, functionality, and viability of biological cells using label-free and contact-free sorting, have thus far not been successfully developed to isolate rare CTCs using clinical samples from cancer patients owing to technical constraints, insufficient throughput, and lack of long-term device stability. In this work, we demonstrate the development of an acoustic-based microfluidic device that is capable of high-throughput separation of CTCs from peripheral blood samples obtained from cancer patients. Our method uses tilted-angle standing surface acoustic waves. Parametric numerical simulations were performed to design optimum device geometry, tilt angle, and cell throughput that is more than 20 times higher than previously possible for such devices. We first validated the capability of this device by successfully separating low concentrations (∼100 cells/mL) of a variety of cancer cells from cell culture lines from WBCs with a recovery rate better than 83%. We then demonstrated the isolation of CTCs in blood samples obtained from patients with breast cancer. Our acoustic-based separation method thus offers the potential to serve as an invaluable supplemental tool in cancer research, diagnostics, drug efficacy assessment, and therapeutics owing to its excellent biocompatibility, simple design, and label-free automated operation while offering the capability to isolate rare CTCs in a viable state.

scholarly journals A frogspawn-like Ag@C core–shell structure for an ultrasensitive label-free electrochemical immunosensing of carcinoembryonic antigen in blood plasma

RSC Advances ◽  
2021 ◽  
Vol 11 (27) ◽  
pp. 16339-16350
Author(s):  
Mengkui Ding ◽  
Ling Zha ◽  
Hui Wang ◽  
Jinyao Liu ◽  
Peiwu Chen ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Carcinoembryonic Antigen ◽  
Human Blood ◽  
Shell Structure ◽  
Core Shell ◽  
Label Free ◽  
Blood Samples ◽  
Human Blood Samples ◽  
Core Shell Structure ◽  
Electrochemical Immunosensing

Novel frogspawn-like Ag@C nanoparticles were successfully used to fabricate an ultrasensitive electrochemical immunosensing platform toward CEA in human blood samples.

scholarly journals Erratum: CORRIGENDUM: Separable Bilayer Microfiltration Device for Viable Label-free Enrichment of Circulating Tumour Cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ming-Da Zhou ◽  
Sijie Hao ◽  
Anthony J. Williams ◽  
Ramdane A. Harouaka ◽  
Brett Schrand ◽  
...  
Keyword(s):  
Tumour Cells ◽  
Circulating Tumour Cells ◽  
Label Free ◽  
Free Enrichment

scholarly journals A new anion exchange purification method for Cu stable isotopes in blood samples

2018 ◽  
Vol 411 (3) ◽  
pp. 765-776 ◽  
Author(s):  
Shun-Chung Yang ◽  
Lisa Welter ◽  
Anand Kolatkar ◽  
Jorge Nieva ◽  
Kathryn R. Waitman ◽  
...  
Keyword(s):  
Stable Isotopes ◽  
Anion Exchange ◽  
Purification Method ◽  
Blood Samples

A PCR-free technology to detect and quantify microRNAs directly from human plasma

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5676-5682 ◽  
Author(s):  
Antonio Marín-Romero ◽  
Agustín Robles-Remacho ◽  
Mavys Tabraue-Chávez ◽  
Bárbara López-Longarela ◽  
Rosario M. Sánchez-Martín ◽  
...  
Keyword(s):  
Human Plasma ◽  
Absolute Quantification ◽  
Label Free ◽  
Blood Samples ◽  
Label Free Detection ◽  
Unique Method

A novel and unique method for a PCR/label-free detection and absolute quantification of miR-451a in blood samples.

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