scholarly journals Intelligent ECM mimetic injectable scaffolds based on functional collagen building blocks for tissue engineering and biomedical applications

RSC Advances ◽  
2017 ◽  
Vol 7 (34) ◽  
pp. 21068-21078 ◽  
Author(s):  
R. Ravichandran ◽  
C. Astrand ◽  
H. K. Patra ◽  
Anthony P. F. Turner ◽  
V. Chotteau ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Biomedical Applications ◽  
Building Blocks ◽  
Ph Responsive ◽  
One Pot ◽  
Injectable Scaffolds ◽  
Responsive Polymer ◽  
Crosslinked Networks ◽  
Responsive Hydrogels

A one-pot approach to fabricate in situ-gellable, thermo- and pH-responsive, hydrogels based on covalently crosslinked networks of collagen-I and thermo-responsive polymer.

Symmetrical tetra-β″-sulfoleno-meso-aryl-porphyrins — synthesis, spectroscopy and structural characterization

2014 ◽  
Vol 18 (01n02) ◽  
pp. 115-122 ◽  
Author(s):  
Srinivas Banala ◽  
Klaus Wurst ◽  
Bernhard Kräutler
Keyword(s):  
Metal Complexes ◽  
Crystal Structures ◽  
Structural Characterization ◽  
Building Blocks ◽  
Cycloaddition Reactions ◽  
One Pot

We report here the preparation (in "one-pot") of a tetra-β″-sulfoleno-meso-aryl-porphyrin in about 80% yield by using an optimized modification of Lindsey's variant of the Adler–Longo approach. The Zn ( II )-, Cu ( II )- and Ni ( II )-complexes of the symmetrical porphyrin were prepared and characterized spectroscopically. Crystal structures of the fluorescent Zn ( II )- and of the non-fluorescent Ni ( II )-tetra-β″-sulfoleno-meso-aryl-porphyrinates showed the highly substituted porphyrin ligands to be nearly perfectly planar. The Zn ( II )-complex of this porphyrin has been used as a thermal precursor of a reactive diene, and — formally — of lateral and diagonal bis-dienes, of a tris-diene and of a tetra-diene, which all underwent [4 + 2]-cycloaddition reactions in situ with a range of dienophiles. Thus, the tetra-β″-sulfoleno-meso-aryl-porphyrin and its metal complexes represent reactive building blocks, "programmed" for the syntheses of symmetrical and highly functionalized porphyrins.

One-pot three-component polymerization for in situ generation of AIE-active poly(tetraarylethene)s using Grignard reagents as building blocks

2020 ◽  
Vol 11 (35) ◽  
pp. 5601-5609
Author(s):  
Zijie Qiu ◽  
Qingqing Gao ◽  
Ting Han ◽  
Xiaolin Liu ◽  
Jacky W. Y. Lam ◽  
...  
Keyword(s):  
Building Blocks ◽  
Grignard Reagents ◽  
One Pot ◽  
Aggregation Induced Emission ◽  
In Situ Generation

A facile polymerization route for in situ generation of polymers with aggregation-induced emission (AIE) characteristics has been developed.

scholarly journals Highly deformable hydrogels constructed by pH-triggered polyacid nanoparticle disassembly in aqueous dispersions

Soft Matter ◽  
2018 ◽  
Vol 14 (18) ◽  
pp. 3510-3520 ◽  
Author(s):  
Wenkai Wang ◽  
Dongdong Lu ◽  
Mingning Zhu ◽  
Jennifer M. Saunders ◽  
Amir H. Milani ◽  
...  
Keyword(s):  
Small Molecule ◽  
Building Blocks ◽  
Polymer Nanoparticles ◽  
Ph Responsive ◽  
Aqueous Dispersions ◽  
Responsive Hydrogels

We study remarkably deformable and resilient pH-responsive hydrogels constructed using crosslinker-free polymer nanoparticles as the only gel building blocks in the absence of added small molecule monomers.

Fabrication of Genetically Engineered Silk-Elastin-Like Protein Polymer Fibers

2008 ◽  
Author(s):  
Weiguo Qiu ◽  
Joseph Cappello ◽  
Xiaoyi Wu
Keyword(s):  
Tissue Engineering ◽  
Biomedical Applications ◽  
Recombinant Dna ◽  
Building Blocks ◽  
Genetically Engineered ◽  
Polymer Fibers ◽  
Structural Support ◽  
Protein Polymer ◽  
Protein Fibers ◽  
Cellular Matrices

Micro- and submicro-diameter protein fibers are fundamental building blocks of extra- and intra-cellular matrices, providing structural support, stability and protection to cells, tissues and organism [1]. Fabricating performance fibers of both naturally derived and genetically engineered proteins has been extensively pursued for a variety of biomedical applications, including tissue engineering and drug delivery [2]. Silk-elastin-like proteins (SELPs), consisting of tandemly repeated polypeptide sequences derived from silk and elastin, have been biosynthesized using recombinant DNA technique [3]. Their potential as a biomaterials in the form of hydrogels continues to be explored [4, 5]. This study will focus on the fabrication of robust, micro-diameter SELP fibers as biomaterials for tissue engineering applications.

A New Synergic-Assembly Strategy Towards Three-Dimensional (3D) Hollow Nanoarchitectures

2008 ◽  
Vol 8 (12) ◽  
pp. 6208-6222 ◽  
Author(s):  
Changzheng Wu ◽  
Yi Xie
Keyword(s):  
Large Scale ◽  
Three Dimensional ◽  
Building Blocks ◽  
Growth Direction ◽  
The Self ◽  
Assembly Process ◽  
Next Generation ◽  
One Pot ◽  
Assembly Strategy

Large-scale synthesis and assembly of meso-, micro- and nanostructured building blocks with the desired orientations are of great interest for the next-generation nanoarchitecture design. On the consideration that the traditional synthetic methodologies for nanostructures often produce tangled nanounits, how to align the nanounits into the ordered orientation at high production yield is a great challenge to current methods. The present review describes a facile and controllable way to grow and assemble the 3D hollow nanoarchitectures, with the utilization of the synergic effects of hollowing process from the self-produced templates and the highly anisotropic growth of nanounits of the target materials in one-pot reaction. In this process, the building block nanounits spontaneously in-situ form owing to their highly anisotropic internal structure, while the self-produced templates act as the supporter and growth-direction guidance for the in-situ formed nanounits. Therefore, the whole assembly process is simple, controllable and without the complicated manipulations. Herein, in the light of the different kinds of self-produced templates involved in the assembly process, recent developments based on the new synergic-assembly strategy are reviewed according to the classifications: (1) self-produced gas bubble template strategy; (2) self-produced homogeneous solid template strategy; (3) self-produced heterogeneous solid template strategy. Notably, the synergic-assembly methodology described in this review provides a newly essential way to construct and assemble nanoarchitectures facilely and controllably, and is also a crucial step for the next-generation of nanoarchitecture design in the near future. In conclusion, the challenges and prospects for the future are discussed.

scholarly journals Smart/stimuli-responsive hydrogels: Cutting-edge platforms for tissue engineering and other biomedical applications

2021 ◽  
pp. 100186
Author(s):  
Hussein M. El-Husseiny ◽  
Eman A. Mady ◽  
Lina Hamabe ◽  
Amira Abugomaa ◽  
Kazumi Shimada ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Biomedical Applications ◽  
Cutting Edge ◽  
Stimuli Responsive ◽  
Responsive Hydrogels

One-Pot Preparation of Stable Organoboronate Reagents for the Functionalization of Unsaturated Four- and Five-Membered Carbo­- and Heterocycles

Synthesis ◽  
2018 ◽  
Vol 50 (16) ◽  
pp. 3149-3160 ◽  
Author(s):  
Dorian Didier ◽  
Andreas Baumann ◽  
Michael Eisold ◽  
Arif Music
Keyword(s):  
Room Temperature ◽  
Functional Group ◽  
Building Blocks ◽  
Boronic Acids ◽  
Ex Situ ◽  
One Pot ◽  
Potential Applicability ◽  
Facile Preparation

Combining a facile preparation of organoboronates with their remarkable stability and functional group tolerance allows for the straightforward synthesis of four- and five-membered carbo- and hetero­cycles. While most strategies rely on the ex situ preparation of boronic acids as isolated intermediates, we demonstrate that in situ transmetalation of sensitive organometallics with boron alkoxides can lead to great stabilization of such species at room temperature. A considerable extension of the library of unsaturated strained structures is achieved through these sequences, expanding the potential applicability of such unusual building blocks.

scholarly journals Synthesis and Characterization of Oxidized Polysaccharides for In Situ Forming Hydrogels

Biomolecules ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1185
Author(s):  
Muhammad Muhammad ◽  
Christian Willems ◽  
Julio Rodríguez-Fernández ◽  
Gloria Gallego-Ferrer ◽  
Thomas Groth
Keyword(s):  
Tissue Engineering ◽  
Building Blocks ◽  
Molecular Weights ◽  
In Situ Forming ◽  
In Situ Forming Hydrogels ◽  
Imine Bond ◽  
Aldehyde Groups ◽  
Soft Gels ◽  
In Situ Gelling

Polysaccharides are widely used as building blocks of scaffolds and hydrogels in tissue engineering, which may require their chemical modification to permit crosslinking. The goal of this study was to generate a library of oxidized alginate (oALG) and oxidized hyaluronic acid (oHA) that can be used for in situ gelling hydrogels by covalent reaction between aldehyde groups of the oxidized polysaccharides (oPS) and amino groups of carboxymethyl chitosan (CMC) through imine bond formation. Here, we studied the effect of sodium periodate concentration and reaction time on aldehyde content, molecular weight of derivatives and cytotoxicity of oPS towards 3T3-L1 fibroblasts. It was found that the molecular weights of all oPs decreased with oxidation and that the degree of oxidation was generally higher in oHA than in oALG. Studies showed that only oPs with an oxidation degree above 25% were cytotoxic. Initial studies were also done on the crosslinking of oPs with CMC showing with rheometry that rather soft gels were formed from higher oxidized oPs possessing a moderate cytotoxicity. The results of this study indicate the potential of oALG and oHA for use as in situ gelling hydrogels or inks in bioprinting for application in tissue engineering and controlled release.

scholarly journals In situ miRNA delivery from a hydrogel promotes osteogenesis of encapsulated mesenchymal stromal cells

2019 ◽  
Author(s):  
James. Carthew ◽  
Surakshya. Shrestha ◽  
John. S. Forsythe ◽  
Ilze. Donderwinkel ◽  
Vinh. X. Truong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Stromal Cells ◽  
Biological Tissues ◽  
Osteogenic Potential ◽  
Great Promise ◽  
One Pot ◽  
Factors Affecting

AbstractHydrogels have many properties that emulate biological tissues and are therefore attractive candidates for use in tissue engineering. In particular the encapsulation and subsequent differentiation of mesenchymal stem/stromal cells (MSCs) is a strategy that holds great promise for the repair and regeneration of bone and cartilage. However, MSCs are well-known for their sensitivity to mechanical cues, particularly substrate stiffness, and so the inherent softness of hydrogels is poorly matched to the mechanical cues that drive efficient osteogenesis. This limits the success of bone tissue engineering using MSCs encapsulated in a hydrogel. One approach to overcome this limitation is to harness mechanotransductive signalling pathways and override the signals cells receive from their environment. Previous reports have shown that the mechanosensitive miRNAs, miR-100-5p and miR-143-3p can enhance MSC osteogenesis, but this required a complex multi-step procedure to transfect, encapsulate and differentiate the cells. In this study, we develop and characterise a facile system for in situ transfection of MSCs encapsulated within a light-crosslinkable gelatin-PEG hydrogel. Comparing the influence of different transfection agents and hydrogel compositions, we determine the factors affecting transfection agent release and MSC transfection, showing that it is possible to transfect MSCs with miRNAs in situ. We then compare the efficacy of both pretransfection and in situ transfection on the osteogenic capacity of hydrogel-encapsulated MSCs, demonstrating superior mineralisation and osteogenic gene expression for in situ transfected samples. Our platform therefore demonstrates a simple, one-pot system for delivery of pro-osteogenic miRNAs and in situ transfection that is able to enhance MSC osteogenic potential without the need of multi-step transfection procedures, thus demonstrating significant promise for bone tissue engineering.

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