scholarly journals Emission enhancement of GFP chromophore in aggregated state via combination of self-restricted effect and supramolecular host–guest complexation

RSC Advances ◽  
2017 ◽  
Vol 7 (29) ◽  
pp. 17980-17987 ◽  
Author(s):  
Shanshan Ge ◽  
Hongping Deng ◽  
Yue Su ◽  
Xinyuan Zhu
Keyword(s):  
Conformational Motion ◽  
Emission Enhancement ◽  
Gfp Chromophore

The emission response of GFP chromophore in aggregated state is greatly enhanced more than 100-fold due to the inhibition of conformational motion and the reduction of strong π–π interaction.

Emission enhancement and application of synthetic green fluorescent protein chromophore analogs

2017 ◽  
Vol 1 (4) ◽  
pp. 619-629 ◽  
Author(s):  
Hongping Deng ◽  
Xinyuan Zhu
Keyword(s):  
Green Fluorescent Protein ◽  
Chemical Modification ◽  
Fluorescent Protein ◽  
Conformational Motion ◽  
Emission Enhancement ◽  
Green Fluorescent

Emission enhancement and application of GFPc analogs are achieved via chemical modification or physical encapsulation due to conformational motion inhibition.

Size-dependent emission of a dipole coupled to a metal nanoparticle

MRS Advances ◽  
2020 ◽  
Vol 5 (62) ◽  
pp. 3315-3325
Author(s):  
Viktoriia Savchuk ◽  
Arthur R. Knize ◽  
Pavlo Pinchuk ◽  
Anatoliy O. Pinchuk
Keyword(s):  
Numerical Analysis ◽  
Quantum Yield ◽  
Electric Dipole ◽  
Incident Radiation ◽  
Metal Nanoparticle ◽  
Plasmonic Nanoparticle ◽  
Size Dependent ◽  
Emission Enhancement ◽  
Electric Dipoles

AbstractWe present a systematic numerical analysis of the quantum yield of an electric dipole coupled to a plasmonic nanoparticle. We observe that the yield is highly dependent on the distance between the electric dipole and the nanoparticle, the size and permittivity of the nanoparticle, and the wavelength of the incident radiation. Our results indicate that enhancement of the quantum yield is only possible for electric dipoles coupled to a nanoparticle with a radius of 20 nm or larger. As the size of the nanoparticle is increased, emission enhancement occurs at wavelengths dependent on the coupling distance.

Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins

2019 ◽  
Author(s):  
Jeffrey Chang ◽  
Matthew Romei ◽  
Steven Boxer
Keyword(s):  
Rational Design ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Super Resolution ◽  
Unit Cell Size ◽  
Chlorine Substituent ◽  
Gfp Chromophore ◽  
The One ◽  
Green Fluorescent ◽  
Super Resolution Microscopy

<p>Double-bond photoisomerization in molecules such as the green fluorescent protein (GFP) chromophore can occur either via a volume-demanding one-bond-flip pathway or via a volume-conserving hula-twist pathway. Understanding the factors that determine the pathway of photoisomerization would inform the rational design of photoswitchable GFPs as improved tools for super-resolution microscopy. In this communication, we reveal the photoisomerization pathway of a photoswitchable GFP, rsEGFP2, by solving crystal structures of <i>cis</i> and <i>trans</i> rsEGFP2 containing a monochlorinated chromophore. The position of the chlorine substituent in the <i>trans</i> state breaks the symmetry of the phenolate ring of the chromophore and allows us to distinguish the two pathways. Surprisingly, we find that the pathway depends on the arrangement of protein monomers within the crystal lattice: in a looser packing, the one-bond-flip occurs, whereas in a tighter packing (7% smaller unit cell size), the hula-twist occurs.</p><p> </p><p> </p><p> </p><p> </p><p> </p><p> </p> <p> </p>

scholarly journals Pyrene-Fused Hexaarylbenzene Luminogens: Synthesis, Characterization, and Aggregation-Induced Emission Enhancement

2021 ◽  
pp. 109452
Author(s):  
Chuan-Zeng Wang ◽  
Ze-Dong Yu ◽  
Wen-Xuan Zhao ◽  
Kai Yang ◽  
Yuki Noda ◽  
...  
Keyword(s):  
Aggregation Induced Emission ◽  
Emission Enhancement

Dual-Self-Restricted GFP Chromophore Analogues with Significantly Enhanced Emission

2020 ◽  
Vol 124 (5) ◽  
pp. 871-880 ◽  
Author(s):  
Hongping Deng ◽  
Chunyang Yu ◽  
Deyue Yan ◽  
Xinyuan Zhu
Keyword(s):  
Gfp Chromophore

scholarly journals Multifold Emission Enhancement in Nanoimprinted Hybrid Perovskite Metasurfaces

ACS Photonics ◽  
2017 ◽  
Vol 4 (4) ◽  
pp. 728-735 ◽  
Author(s):  
Sergey V. Makarov ◽  
Valentin Milichko ◽  
Elena V. Ushakova ◽  
Mikhail Omelyanovich ◽  
Andrea Cerdan Pasaran ◽  
...  
Keyword(s):  
Hybrid Perovskite ◽  
Emission Enhancement

The Case of the Missing Ring:  Radical Cleavage of a Carbon−Carbon Bond and Implications for GFP Chromophore Biosynthesis

2007 ◽  
Vol 129 (11) ◽  
pp. 3118-3126 ◽  
Author(s):  
David P. Barondeau ◽  
Carey J. Kassmann ◽  
John A. Tainer ◽  
Elizabeth D. Getzoff
Keyword(s):  
Carbon Bond ◽  
Carbon Carbon Bond ◽  
Gfp Chromophore ◽  
Radical Cleavage ◽  
Missing Ring
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