Photo-induced DNA cleavage by zinc-substituted myoglobin with a redesigned active center

2017 ◽  
Vol 4 (12) ◽  
pp. 2033-2036 ◽  
Author(s):  
Zhen-Hua Shi ◽  
Ke-Jie Du ◽  
Bo He ◽  
Shu-Qin Gao ◽  
Ge-Bo Wen ◽  
...  
Keyword(s):  
Active Center ◽  
Dna Cleavage ◽  
Zinc Protoporphyrin ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Artificial Nucleases

Artificial nucleases were constructed by the redesign of the heme center in myoglobin (Mb) and replacement of the native heme with zinc protoporphyrin (ZnPP), which exhibit tunable photo-induced DNA cleavage activity.

Promotive Effect of the Platinum Moiety on the DNA Cleavage Activity of Copper-Based Artificial Nucleases

2010 ◽  
Vol 49 (5) ◽  
pp. 2541-2549 ◽  
Author(s):  
Xindian Dong ◽  
Xiaoyong Wang ◽  
Miaoxin Lin ◽  
Hui Sun ◽  
Xiaoliang Yang ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Artificial Nucleases ◽  
Promotive Effect

Synthesis, Structure, and DNA Cleavage Activity of New Trinuclear Zn3and Zn2Cu Complexes of a Chiral Macrocycle:  Structural Correlation with the Active Center of P1 Nuclease

2002 ◽  
Vol 41 (16) ◽  
pp. 4099-4101 ◽  
Author(s):  
Srinivas R. Korupoju ◽  
Nagarathinam Mangayarkarasi ◽  
Panthapally S. Zacharias ◽  
Jun Mizuthani ◽  
Hiroshi Nishihara
Keyword(s):  
Active Center ◽  
Dna Cleavage ◽  
Cleavage Activity ◽  
Structural Correlation ◽  
Dna Cleavage Activity

DNA-cleavage activity of the iron(II) complex with optically active ligands, meta- and para-xylyl-linked N’,N’-dipyridylmethyl-cyclohexane-1,2-diamine

2021 ◽  
Vol 36 ◽  
pp. 127834
Author(s):  
Koichi Kato ◽  
Yoshimi Ichimaru ◽  
Yoshinori Okuno ◽  
Yoshihiro Yamaguchi ◽  
Wanchun Jin ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Optically Active ◽  
Cleavage Activity ◽  
Dna Cleavage Activity

scholarly journals A YoeB toxin cleaves both RNA and DNA

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Julia McGillick ◽  
Jessica R. Ames ◽  
Tamiko Murphy ◽  
Christina R. Bourne
Keyword(s):  
Dna Cleavage ◽  
Divalent Cations ◽  
Nuclease Activity ◽  
Dose Dependence ◽  
Cleavage Activity ◽  
Double Stranded Dna ◽  
Dna Cleavage Activity ◽  
Evolutionarily Conserved ◽  
Toxin Protein ◽  
Rna And Dna

AbstractType II toxin-antitoxin systems contain a toxin protein, which mediates diverse interactions within the bacterial cell when it is not bound by its cognate antitoxin protein. These toxins provide a rich source of evolutionarily-conserved tertiary folds that mediate diverse catalytic reactions. These properties make toxins of interest in biotechnology applications, and studies of the catalytic mechanisms continue to provide surprises. In the current work, our studies on a YoeB family toxin from Agrobacterium tumefaciens have revealed a conserved ribosome-independent non-specific nuclease activity. We have quantified the RNA and DNA cleavage activity, revealing they have essentially equivalent dose-dependence while differing in requirements for divalent cations and pH sensitivity. The DNA cleavage activity is as a nickase for any topology of double-stranded DNA, as well as cleaving single-stranded DNA. AtYoeB is able to bind to double-stranded DNA with mid-micromolar affinity. Comparison of the ribosome-dependent and -independent reactions demonstrates an approximate tenfold efficiency imparted by the ribosome. This demonstrates YoeB toxins can act as non-specific nucleases, cleaving both RNA and DNA, in the absence of being bound within the ribosome.

scholarly journals Correction to Copper(II) Complexes of l-Arginine as Netropsin Mimics Showing DNA Cleavage Activity in Red Light

2019 ◽  
Vol 58 (19) ◽  
pp. 13502-13503
Author(s):  
Ashis K. Patra ◽  
Tuhin Bhowmick ◽  
Sovan Roy ◽  
Suryanarayanarao Ramakumar ◽  
Akhil R. Chakravarty
Keyword(s):  
Dna Cleavage ◽  
Red Light ◽  
Cleavage Activity ◽  
Dna Cleavage Activity

Cytotoxic copper (II) mixed ligand complexes: Crystal structure and DNA cleavage activity

2011 ◽  
Vol 46 (9) ◽  
pp. 4537-4547 ◽  
Author(s):  
Verasuntharam M. Manikandamathavan ◽  
Royapuram P. Parameswari ◽  
Thomas Weyhermüller ◽  
Hannah R. Vasanthi ◽  
Balachandran Unni Nair
Keyword(s):  
Crystal Structure ◽  
Dna Cleavage ◽  
Mixed Ligand ◽  
Mixed Ligand Complexes ◽  
Cleavage Activity ◽  
Dna Cleavage Activity

Extra Copper-mediated Enhancement of the DNA Cleavage Activity Supported with Wild-type Cu, Zn Superoxide Dismutase

2008 ◽  
Vol 26 (3) ◽  
pp. 564-570 ◽  
Author(s):  
Ruo-Yu ZHOU ◽  
Wei JIANG ◽  
Li-Na ZHANG ◽  
Li WANG ◽  
Chang-Lin LIU
Keyword(s):  
Superoxide Dismutase ◽  
Dna Cleavage ◽  
Wild Type ◽  
Cleavage Activity ◽  
Dna Cleavage Activity
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