Isoafricanol synthase from Streptomyces malaysiensis

2017 ◽  
Vol 15 (11) ◽  
pp. 2353-2358 ◽  
Author(s):  
Patrick Rabe ◽  
Markiyan Samborskyy ◽  
Peter F. Leadlay ◽  
Jeroen S. Dickschat
Keyword(s):  
Streptomyces Malaysiensis

A terpene cyclases from Streptomyces malaysiensis was characterised as (+)-isoafricanol synthase and its mechanism was investigated using isotopically labelled substrates.

A novel strain of Streptomyces malaysiensis isolated from Brazilian soil produces high endo- -1,4-xylanase titres

2003 ◽  
Vol 19 (9) ◽  
pp. 879-881 ◽  
Author(s):  
Rodrigo Pires do Nascimento ◽  
Susana Marques ◽  
Luís Alves ◽  
Francisco Gírio ◽  
Maria Teresa Amaral-Collaço ◽  
...  
Keyword(s):  
Streptomyces Malaysiensis

scholarly journals A Thermotolerant Xylan-Degrading Enzyme Is Produced by Streptomyces malaysiensis AMT-3 Using by-Products From the Food Industry

2020 ◽  
Vol 63 ◽  
Author(s):  
Rodrigo Pires do Nascimento ◽  
Alberto Delgado Reis ◽  
Francisco Gírio ◽  
Nei Pereira Jr ◽  
Elba Pinto da Silva Bon ◽  
...  
Keyword(s):  
Food Industry ◽  
Degrading Enzyme ◽  
Streptomyces Malaysiensis ◽  
By Products

Exemplar Abstract for Streptomyces malaysiensis Al-Tai et al. 1999.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Streptomyces Malaysiensis

scholarly journals Sulfation and amidinohydrolysis in the biosynthesis of giant linear polyenes

2017 ◽  
Vol 13 ◽  
pp. 2408-2415 ◽  
Author(s):  
Hui Hong ◽  
Markiyan Samborskyy ◽  
Katsiaryna Usachova ◽  
Katharina Schnatz ◽  
Peter F Leadlay
Keyword(s):  
Escherichia Coli ◽  
Gene Cluster ◽  
Late Stage ◽  
Rare Case ◽  
Gene Clusters ◽  
Biosynthetic Gene ◽  
Starter Unit ◽  
Linear Polyenes ◽  
Streptomyces Malaysiensis

Clethramycin from Streptomyces malaysiensis DSM4137, and mediomycins (produced together with clethramycin from Streptomyces mediocidicus), are near-identical giant linear polyenes apparently constructed from, respectively, a 4-guanidinobutanoate or 4-aminobutanoate starter unit and 27 polyketide extender units, and bearing a specific O-sulfonate modification at the C-29 hydroxy group. We show here that mediomycins are actually biosynthesised not by use of a different starter unit but by direct late-stage deamidination of (desulfo)clethramycin. A gene (slf) encoding a candidate sulfotransferase has been located in both gene clusters. Deletion of this gene in DSM4137 led to accumulation of desulfoclethramycin only, instead of a mixture of desulfoclethramycin and clethramycin. The mediomycin gene cluster does not encode an amidinohydrolase, but when three candidate amidinohydrolase genes from elsewhere in the S. mediocidicus genome were individually expressed in Escherichia coli and assayed, only one of them (medi4948), located 670 kbp away from the mediomycin gene cluster on the chromosome, catalysed the removal of the amidino group from desulfoclethramycin. Subsequent cloning of medi4948 into DSM4137 caused mediomycins A and B to accumulate at the expense of clethramycin and desulfoclethramycin, respectively, a rare case where an essential biosynthetic gene is not co-located with other pathway genes. Clearly, both desulfoclethramycin and clethramycin are substrates for this amidinohydrolase. Also, purified recombinant sulfotransferase from DSM4137, in the presence of 3'-phosphoadenosine-5'-phosphosulfate as donor, efficiently converted mediomycin B to mediomycin A in vitro. Thus, in the final steps of mediomycin A biosynthesis deamidination and sulfotransfer can take place in either order.

Total Synthesis and Structural Confirmation of Malayamycn A: A Novel Bicyclic C-Nucleoside from Streptomyces malaysiensis.

ChemInform ◽  
2004 ◽  
Vol 35 (12) ◽  
Author(s):  
Stephen Hanessian ◽  
Stephane Marcotte ◽  
Roger Machaalani ◽  
Guobin Huang
Keyword(s):  
Total Synthesis ◽  
Streptomyces Malaysiensis

Efophylins A and B, Two C2-Asymmetric Macrodiolide Immunosuppressants from Streptomyces malaysiensis

2021 ◽  
Author(s):  
Jing Xu ◽  
Xuexia Zhang ◽  
Fanglu Huang ◽  
Gang Li ◽  
Peter F. Leadlay
Keyword(s):  
Streptomyces Malaysiensis

Identification of 2-MIB and geosmin producers in Feng-Shen reservoir in south Taiwan

2006 ◽  
Vol 6 (2) ◽  
pp. 55-61 ◽  
Author(s):  
S.-C. Tung ◽  
T.-F. Lin ◽  
I.-C. Tseng ◽  
H.-M. Lin
Keyword(s):  
16S Rdna ◽  
Incubation Temperature ◽  
Rdna Sequence ◽  
Source Water ◽  
16S Rdna Sequence ◽  
Streptomyces Malaysiensis

Musty and earthy odor are present in the source water of Feng-Shen waterworks (FSW) in south Taiwan all year round. Although 2-methylisoborneol (2-MIB) and geosmin are responsible for the musty and earthy odor, respectively, the possible odor producers remained unknown. In this study, actinomycetes that produce 2-MIB and geosmin were studied. Based on the 16S rDNA sequence, three species were identified after comparison with the GenBank database at the NCBI. The three species are Streptomyces malaysiensis as a 2-MIB and geosmin producer, Streptomyces caelestis as a 2-MIB producer, and Streptomyces roseoflavus as a geosmin producer. For S. malaysiensis and S. caelestis, the production of MIB and/or geosmin was increased with incubation temperature (20–30 °C) in starch–glutamate broth medium. The optimal MIB, geosmin and biomass occurred at 30 °C. The maximal geosmin/biomass (G/B) and/or MIB/biomass (M/B) also occurred at 30 °C, and sporulating cultures contained more geosmin and/or MIB than nonsporulatig cultures.

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