Real-time monitoring of the Trojan-horse effect of silver nanoparticles by using a genetically encoded fluorescent cell sensor

Nanoscale ◽  
2018 ◽  
Vol 10 (16) ◽  
pp. 7726-7735 ◽  
Author(s):  
Fang You ◽  
Wenqin Tang ◽  
Lin-Yue Lanry Yung
Keyword(s):  
Silver Nanoparticles ◽  
Real Time ◽  
Fluorescent Protein ◽  
Trojan Horse ◽  
Real Time Monitoring ◽  
Fluorescent Cell ◽  
Protein Sensor

A genetically encoded fluorescent protein sensor enabled monitoring the “Trojan-horse” type cytotoxicity of silver nanoparticles.

Live demonstration: A smart camera for real-time monitoring of fluorescent cell biomarkers

2014 ◽  
Author(s):  
Julien Ghaye ◽  
Sinan K. Muldur ◽  
Patricia Urban ◽  
Agnieszka Kinsner-Ovaskainen ◽  
Pascal Colpo ◽  
...  
Keyword(s):  
Real Time ◽  
Smart Camera ◽  
Real Time Monitoring ◽  
Fluorescent Cell ◽  
Live Demonstration

Real-time monitoring of the dissolution of silver nanoparticles by using a solid-contact Ag+-selective electrode

2020 ◽  
Vol 1101 ◽  
pp. 50-57 ◽  
Author(s):  
Tanji Yin ◽  
Tingting Han ◽  
Changbai Li ◽  
Wei Qin ◽  
Johan Bobacka
Keyword(s):  
Silver Nanoparticles ◽  
Real Time ◽  
Real Time Monitoring ◽  
Solid Contact ◽  
Selective Electrode

Real-time monitoring of the dissolution kinetics of silver nanoparticles and nanowires in aquatic environments using an aggregation-induced emission fluorogen

2018 ◽  
Vol 54 (36) ◽  
pp. 4585-4588 ◽  
Author(s):  
Neng Yan ◽  
Sheng Xie ◽  
Ben Zhong Tang ◽  
Wen-Xiong Wang
Keyword(s):  
Silver Nanoparticles ◽  
Real Time ◽  
Dissolution Kinetics ◽  
Aquatic Environments ◽  
Real Time Monitoring ◽  
Aggregation Induced Emission ◽  
Kinetics Of

We employed a fluorogenic Ag+ sensor, tetrazole-functionalized tetraphenylethylene derivative 1 (TEZ-TPE-1), to investigate the dissolution kinetics of AgNPs and AgNWs in aquatic environments.

scholarly journals Real-Time Optical Detection of Isoleucine in Living Cells through a Genetically-Encoded Nanosensor

Sensors ◽  
2019 ◽  
Vol 20 (1) ◽  
pp. 146
Author(s):  
Shruti Singh ◽  
Maheshwar Prasad Sharma ◽  
Abdulaziz A. Alqarawi ◽  
Abeer Hashem ◽  
Elsayed Fathi Abd_Allah ◽  
...  
Keyword(s):  
Real Time ◽  
Metabolic Regulation ◽  
Metabolic Flux ◽  
Fluorescent Protein ◽  
Animal Feed ◽  
Living Cells ◽  
Yeast Cells ◽  
Human Beings ◽  
Real Time Monitoring ◽  
Enhanced Production

Isoleucine is one of the branched chain amino acids that plays a major role in the energy metabolism of human beings and animals. However, detailed investigation of specific receptors for isoleucine has not been carried out because of the non-availability of a tool that can monitor the metabolic flux of this amino acid in live cells. This study presents a novel genetically-encoded nanosensor for real-time monitoring of isoleucine in living cells. This nanosensor was developed by sandwiching a periplasmic binding protein (LivJ) of E. coli between a fluorescent protein pair, ECFP (Enhanced Cyan Fluorescent Protein), and Venus. The sensor, named GEII (Genetically Encoded Isoleucine Indicator), was pH stable, isoleucine-specific, and had a binding affinity (Kd) of 63 ± 6 μM. The GEII successfully performed real-time monitoring of isoleucine in bacterial and yeast cells, thereby, establishing its bio-compatibility in monitoring isoleucine in living cells. As a further enhancement, in silico random mutagenesis was carried out to identify a set of viable mutations, which were subsequently experimentally verified to create a library of affinity mutants with a significantly expanded operating range (96 nM–1493 μM). In addition to its applicability in understanding the underlying functions of receptors of isoleucine in metabolic regulation, the GEII can also be used for metabolic engineering of bacteria for enhanced production of isoleucine in animal feed industries.

scholarly journals Real-Time Monitoring of Chromophore Isomerization and Deprotonation during the Photoactivation of the Fluorescent Protein Dronpa

2014 ◽  
Vol 119 (6) ◽  
pp. 2404-2414 ◽  
Author(s):  
Dheerendra Yadav ◽  
Fabien Lacombat ◽  
Nadia Dozova ◽  
Fabrice Rappaport ◽  
Pascal Plaza ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Protein ◽  
Real Time Monitoring

1618: Real-Time Monitoring of Nitric Oxide and Blood Flow During Ischemia-Reperfusion in the Rat Testis

2006 ◽  
Vol 175 (4S) ◽  
pp. 521-521
Author(s):  
Motoaki Saito ◽  
Tomoharu Kono ◽  
Yukako Kinoshita ◽  
Itaru Satoh ◽  
Keisuke Satoh
Keyword(s):  
Nitric Oxide ◽  
Blood Flow ◽  
Real Time ◽  
Ischemia Reperfusion ◽  
Real Time Monitoring ◽  
Rat Testis
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