scholarly journals Surface-enhanced hyper Raman hyperspectral imaging and probing in animal cells

Nanoscale ◽  
2017 ◽  
Vol 9 (23) ◽  
pp. 8024-8032 ◽  
Author(s):  
Zsuzsanna Heiner ◽  
Marina Gühlke ◽  
Vesna Živanović ◽  
Fani Madzharova ◽  
Janina Kneipp
Keyword(s):  
Hyperspectral Imaging ◽  
Live Cells ◽  
Animal Cells ◽  
Two Photon ◽  
Surface Enhanced

The location of nanoprobes in endosomes of live cells is mapped by off-resonant, two-photon excited vibrational imaging.

scholarly journals Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3758
Author(s):  
Andrea Ceja-Fdez ◽  
Ramon Carriles ◽  
Ana Lilia González-Yebra ◽  
Juan Vivero-Escoto ◽  
Elder de la Rosa ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Rapid Screening ◽  
Emission Region ◽  
Sem Images ◽  
Spectral Signatures ◽  
Two Photon ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Gold Nps

In this work, gold NPs were prepared by the Turkevich method, and their interaction with HPV and cancerous cervical tissues were studied by scanning electron microscopy, energy-dispersive x-ray spectroscopy, confocal and multiphoton microscopy and SERS. The SEM images confirmed the presence and localization of the gold NPs inside of the two kinds of tissues. The light absorption of the gold NPs was at 520 nm. However, it was possible to obtain two-photon imaging (red emission region) of the gold NPs inside of the tissue, exciting the samples at 900 nm, observing the morphology of the tissues. The infrared absorption was probably due to the aggregation of gold NPs inside the tissues. Therefore, through the interaction of gold nanoparticles with the HPV and cancerous cervical tissues, a surface enhanced Raman spectroscopy (SERS) was obtained. As preliminary studies, having an average of 1000 Raman spectra per tissue, SERS signals showed changes between the HPV-infected and the carcinogenic tissues; these spectral signatures occurred mainly in the DNA bands, potentially offering a tool for the rapid screening of cancer.

A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells

2017 ◽  
Vol 53 (81) ◽  
pp. 11213-11216 ◽  
Author(s):  
Kondapa Naidu Bobba ◽  
Miae Won ◽  
Inseob Shim ◽  
Nithya Velusamy ◽  
Zhigang Yang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Live Cells ◽  
Two Photon

A two-photon fluorescent probe (Tyro-1) can validate intracellular tyrosinase activity, and H2O2induced upregulated tyrosinase in melanoma cells.

scholarly journals Two-photon activated precision molecular photosensitizer targeting mitochondria

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Inês F. A. Mariz ◽  
Sandra N. Pinto ◽  
Ana M. Santiago ◽  
José M. G. Martinho ◽  
Javier Recio ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Near Infrared ◽  
Therapeutic Potential ◽  
Blue Shift ◽  
Specific Cell ◽  
Animal Cells ◽  
Mitochondrial Potential ◽  
Live Animal ◽  
Two Photon ◽  
Mitochondria Targeting

AbstractMitochondria metabolism is an emergent target for the development of novel anticancer agents. It is amply recognized that strategies that allow for modulation of mitochondrial function in specific cell populations need to be developed for the therapeutic potential of mitochondria-targeting agents to become a reality in the clinic. In this work, we report dipolar and quadrupolar quinolizinium and benzimidazolium cations that show mitochondria targeting ability and localized light-induced mitochondria damage in live animal cells. Some of the dyes induce a very efficient disruption of mitochondrial potential and subsequent cell death under two-photon excitation in the Near-infrared (NIR) opening up possible applications of azonia/azolium aromatic heterocycles as precision photosensitizers. The dipolar compounds could be excited in the NIR due to a high two-photon brightness while exhibiting emission in the red part of the visible spectra (600–700 nm). Interaction with the mitochondria leads to an unexpected blue-shift of the emission of the far-red emitting compounds, which we assign to emission from the locally excited state. Interaction and possibly aggregation at the mitochondria prevents access to the intramolecular charge transfer state responsible for far-red emission.

Rational design of a lysosomal-targeted ratiometric two-photon fluorescent probe for imaging hydrogen polysulfides in live cells

2020 ◽  
Vol 173 ◽  
pp. 107877 ◽  
Author(s):  
Qingxin Han ◽  
Xuan Liu ◽  
Xuechuan Wang ◽  
Ruojun Yin ◽  
Huie Jiang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Rational Design ◽  
Live Cells ◽  
Two Photon

A Type of Atypical AIEgen Used for One-Photon/Two-Photon Targeted Imaging in Live Cells

2019 ◽  
Vol 3 (1) ◽  
pp. 505-511 ◽  
Author(s):  
Kai Sun ◽  
Yin-Li Zhang ◽  
Xiao-Lan Chen ◽  
Hui-Fang Su ◽  
Qiu-Chen Peng ◽  
...  
Keyword(s):  
Live Cells ◽  
Targeted Imaging ◽  
Two Photon

A two-photon ratiometric fluorescent probe for highly selective sensing of mitochondrial cysteine in live cells

The Analyst ◽  
2019 ◽  
Vol 144 (2) ◽  
pp. 439-447 ◽  
Author(s):  
Li Fan ◽  
Wenjia Zhang ◽  
Xiaodong Wang ◽  
Wenjuan Dong ◽  
Yaoli Tong ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Live Cells ◽  
Two Photon ◽  
Ratiometric Fluorescent Probe

We report a novel two-photon ratiometric fluorescent probe (DNEPI) for highly selective sensing mitochondrial Cys in live cells.

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