scholarly journals Evaluation of spectral photon counting computed tomography K-edge imaging for determination of gold nanoparticle biodistribution in vivo

Nanoscale ◽  
2017 ◽  
Vol 9 (46) ◽  
pp. 18246-18257 ◽  
Author(s):  
Salim Si-Mohamed ◽  
David P. Cormode ◽  
Daniel Bar-Ness ◽  
Monica Sigovan ◽  
Pratap C. Naha ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Quantitative Determination ◽  
Gold Nanoparticle ◽  
Photon Counting ◽  
Non Invasive ◽  
Over Time

A new spectral photon-counting CT prototype has the potential for non-invasive quantitative determination of gold nanoparticle biodistribution in vivo over time.

Abstract 15015: Multimodal in vivo Computed Tomography and Optical Imaging for Simultaneous Functional, Metabolic and Molecular Myocardial Analysis

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Elza D van Deel ◽  
Yanto Ridwan ◽  
Sasha Belenkov ◽  
Jeroen Essers
Keyword(s):  
Myocardial Infarction ◽  
Computed Tomography ◽  
Near Infrared ◽  
Left Ventricular ◽  
Ct Imaging ◽  
Diagnostic Methods ◽  
Left Ventricular Ejection ◽  
Non Invasive ◽  
Over Time

Introduction and Hypothesis: The prevalence of myocardial infarction increases with the average age of the population and is currently the leading cause of death worldwide. In order to define anatomical changes and biomarkers related to cardiac infarction, we tested the use of combined computed tomography (CT) and near infrared fluorescent (NIRF) probes to facilitate non-invasive imaging of processes concerned with tissue degeneration/regeneration. This development of new non-invasive diagnostic methods will lead to better treatment options in the future. Methods: Mice were subjected to left anterior descending artery (LAD) ligation inducing an acute myocardial infarction and subsequently imaged in vivo using fast and low dose microCT scanning (QuantumFX, Perkin Elmer) and near infrared (NIRF) probes to monitor MMP activity (MMPsense680). Immediate contrast enhanced CT imaging using eXIA160 during its blood-pool phase allowed registration of changes in ventricular anatomy and function of important global parameters, like end-diastolic volume (EDV), end-systolic volume (ESV). These were used to calculate stroke volume (SV), and left ventricular ejection fraction (LVEF). Delayed eXIA160 contrast uptake during its myocardial phase, subsequently allowed analysis of the infarct size and infarct healing. In addition, in vivo molecular MMPsense localization was combined with micro-CT imaging for accurate 3D co-registration. Results: Changes in ventricular anatomy and myocardial viability were assessed 3 hours and 2 months post LAD occlusion in the same animal, demonstrating the feasibility of monitoring myocard viability over time. The decreased uptake of eXIA160 in the myocardium was subsequently quantitated. A concomitant increase in MMP activity, as determined by fluorescence mediated tomography using MMPsense680, could be localized to the infarcted site. Conclusions: Non-invasive imaging, using NIRF probes, enables longitudinal imaging of processes concerned with myocard infarction. Consequently, disease progression can be monitored over time and the effect of (new) pharmacotherapy can be studied.

scholarly journals Prevalence and Photobiology of Photosynthetic Dinoflagellate Endosymbionts in the Nudibranch Berghia stephanieae

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2200
Author(s):  
Ruben X. G. Silva ◽  
Paulo Cartaxana ◽  
Ricardo Calado
Keyword(s):  
Food Deprivation ◽  
Photosynthetic Efficiency ◽  
Rapid Decrease ◽  
Complete Loss ◽  
Symbiotic Association ◽  
Non Invasive ◽  
Sea Slugs ◽  
Non Destructive ◽  
Over Time

Berghia stephanieae is a stenophagous sea slug that preys upon glass anemones, such as Exaiptasia diaphana. Glass anemones host photosynthetic dinoflagellate endosymbionts that sea slugs ingest when consuming E. diaphana. However, the prevalence of these photosynthetic dinoflagellate endosymbionts in sea slugs appears to be short-lived, particularly if B.stephanieae is deprived of prey that host these microalgae (e.g., during bleaching events impacting glass anemones). In the present study, we investigated this scenario, along with food deprivation, and validated the use of a non-invasive and non-destructive approach employing chlorophyll fluorescence as a proxy to monitor the persistence of the association between sea slugs and endosymbiotic photosynthetic dinoflagellates acquired through the consumption of glass anemones. Berghia stephanieae deprived of a trophic source hosting photosynthetic dinoflagellate endosymbionts (e.g., through food deprivation or by feeding on bleached E. diaphana) showed a rapid decrease in minimum fluorescence (Fo) and photosynthetic efficiency (Fv/Fm) when compared to sea slugs fed with symbiotic anemones. A complete loss of endosymbionts was observed within 8 days, confirming that no true symbiotic association was established. The present work opens a new window of opportunity to rapidly monitor in vivo and over time the prevalence of associations between sea slugs and photosynthetic dinoflagellate endosymbionts, particularly during bleaching events that prevent sea slugs from incorporating new microalgae through trophic interactions.

Pegylated magnetic nanocarriers for doxorubicin delivery: A quantitative determination of stealthiness in vitro and in vivo

2012 ◽  
Vol 81 (3) ◽  
pp. 498-505 ◽  
Author(s):  
E. Allard-Vannier ◽  
S. Cohen-Jonathan ◽  
J. Gautier ◽  
K. Hervé-Aubert ◽  
E. Munnier ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Doxorubicin Delivery ◽  
Magnetic Nanocarriers

Metabolizable dopamine-coated gold nanoparticle aggregates: preparation, characteristics, computed tomography imaging, acute toxicity, and metabolism in vivo

2016 ◽  
Vol 4 (6) ◽  
pp. 1090-1099 ◽  
Author(s):  
Yao Yu ◽  
Youshen Wu ◽  
Jiajun Liu ◽  
Ke Li ◽  
Daocheng Wu
Keyword(s):  
Computed Tomography ◽  
Acute Toxicity ◽  
Gold Nanoparticle ◽  
Circulation Time ◽  
Computed Tomography Imaging ◽  
Lower Toxicity ◽  
Tomography Imaging ◽  
Imaging Results ◽  
Nanoparticle Aggregates

We prepared metabolizable MSA AuNPs@DPA aggregates, which exhibit improved CT imaging results, much lower toxicity and much longer circulation time in vivo.

Quantitative determination of cyanobacteria in mixed phytoplankton assemblages by an in vivo fluorimetric method

1995 ◽  
Vol 302 (1) ◽  
pp. 81-87 ◽  
Author(s):  
Tsai-yun Lee ◽  
Mikio Tsuzuki ◽  
Toshifumi Takeuchi ◽  
Kenji Yokoyama ◽  
Isao Karube
Keyword(s):  
Quantitative Determination ◽  
Phytoplankton Assemblages ◽  
Fluorimetric Method

Quantitative determination of iodine in vivo

1959 ◽  
Vol 52 (5) ◽  
pp. 337-346 ◽  
Author(s):  
Paul Edholm ◽  
Bertil Jacobson
Keyword(s):  
Quantitative Determination
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