Preparation and characterization of universal Fe3O4@SiO2/CdTe nanocomposites for rapid and facile detection and separation of membrane proteins

2018 ◽  
Vol 42 (7) ◽  
pp. 4981-4990 ◽  
Author(s):  
Min Wang ◽  
Ke-yan Zheng ◽  
Shao-wu Lv ◽  
Hai-feng Zou ◽  
Hong-sen Liu ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Cell Membrane Proteins ◽  
Detection And Separation

The separation and enrichment of cell membrane proteins was achieved by the construction of bi-functional magnetic fluorescent nanoprobes.

scholarly journals Characterization of red cell membrane proteins as a function of red cell density:

FEBS Letters ◽  
2006 ◽  
Vol 580 (28-29) ◽  
pp. 6527-6532 ◽  
Author(s):  
Marianna Caterino ◽  
Margherita Ruoppolo ◽  
Stefania Orrù ◽  
Marcella Savoia ◽  
Silverio Perrotta ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Cell Density ◽  
Red Cell ◽  
Red Cell Membrane ◽  
Cell Membrane Proteins

scholarly journals Novel autoantibodies against muscle-cell membrane proteins in patients with myositis

1996 ◽  
Vol 39 (11) ◽  
pp. 1860-1868 ◽  
Author(s):  
Bruno Stuhlmüller ◽  
Ricardo Jerez ◽  
Gert Hausdorf ◽  
Hans-R. Barthel ◽  
Michael Meurer ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Muscle Cell ◽  
Cell Membrane Proteins ◽  
Muscle Cell Membrane

scholarly journals Identification of novel therapeutic targets for histone 3 mutated children’s brain tumour, using unique tumour cell surface proteomic signatures

2021 ◽  
Vol 23 (Supplement_4) ◽  
pp. iv9-iv9
Author(s):  
Anya Snary ◽  
Richard Grundy ◽  
Rob Layfield ◽  
Ruman Rahman ◽  
Farhana Haque
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Cell Surface ◽  
Brain Tumours ◽  
Expression Patterns ◽  
Surface Membrane ◽  
Therapeutic Targets ◽  
Molecular Signature ◽  
Histone 3 ◽  
Cell Membrane Proteins

Abstract Aims Improvements in the treatments for childhood and adolescent brain tumours, High-Grade Glioma (pHGG) and Diffuse Intrinsic Pontine Glioblastoma (DIPG), have not advanced much and they continue to carry a very poor prognosis. These brain tumours are now defined by mutations affecting histone 3 proteins, indeed 80% of DIPGs harbour histone H3.1 and H3.3 K27M somatic mutations whilst 30% of pHGGs exhibit H3.3 G34R or G34V mutations. We hypothesized that the histone 3 mutant tumours will have distinct mutation specific surfactome (cell membrane proteins) signature. Method We therefore analysed the cell surface proteomics of pHGG and DIPG, in order to identify novel targets for therapy. We have at first isolated the cell membrane fractions from a range of patient cells carrying different histone 3 mutations (G34R, G34V), relative to wild type histone 3. A comparative quantitative mass-spectrometry analyses of these cell surface membrane fractions is then performed. Results The results obtained to date demonstrated unique differential cell membrane expression patterns which correlated to specific mutation type. For example, increased expression of Ras-related proteins Rab-3, Rab-3D is detected only in histone H3.3-G34R mutated cell line in comparison. Conclusion Identification and analyses of these unique cell membrane proteins’ association with specific in H3.3 mutation in pHGG, will help to identify precise mutation specific therapeutic targets, benefiting the patients to receive therapy based on tumour’s molecular signature.

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