A cool and high salt-tolerant ionic liquid matrix for preferential ionization of phosphopeptides by negative ion MALDI-MS

2017 ◽  
Vol 41 (20) ◽  
pp. 12241-12249 ◽  
Author(s):  
Ling Ling ◽  
Chunsheng Xiao ◽  
Liyan Jiang ◽  
Sheng Wang ◽  
Ying Li ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
High Salt ◽  
Maldi Ms ◽  
Negative Ion ◽  
Liquid Matrix ◽  
Salt Tolerant

An optimized ILM G3THAP/PA matrix significantly improved the detection of phosphopeptides by negative ion MALDI-MS compared with using 3-AQ/CHCA/ADP and DHB/PA matrices.

Structural Analysis of N-Glycans by the Glycan-Labeling Method Using 3-Aminoquinoline-Based Liquid Matrix in Negative-Ion MALDI-MS

2012 ◽  
Vol 84 (21) ◽  
pp. 9453-9461 ◽  
Author(s):  
Takashi Nishikaze ◽  
Kaoru Kaneshiro ◽  
Shin-ichirou Kawabata ◽  
Koichi Tanaka
Keyword(s):  
Structural Analysis ◽  
Maldi Ms ◽  
Negative Ion ◽  
Liquid Matrix ◽  
Labeling Method

scholarly journals The Culture of Salt-Tolerant Strains and its Degradation Performance of High-salt organic Wastewater

2021 ◽  
Vol 631 ◽  
pp. 012029
Author(s):  
Yinghan Li ◽  
Zheng Luo ◽  
Fengbo Guo ◽  
Kaizhi Zhang ◽  
Qiao Zhou ◽  
...  
Keyword(s):  
High Salt ◽  
Salt Tolerant ◽  
Organic Wastewater

scholarly journals The full-length transcriptome of Spartina alterniflora reveals the complexity of high salt tolerance in monocotyledonous halophyte

2019 ◽  
Author(s):  
Wenbin Ye ◽  
Taotao Wang ◽  
Wei Wei ◽  
Shuaitong Lou ◽  
Faxiu Lan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Salt Stress ◽  
Alternative Splicing ◽  
Salt Tolerance ◽  
Protein Kinases ◽  
Spartina Alterniflora ◽  
High Salt ◽  
Full Length ◽  
Salt Tolerant ◽  
High Salt Tolerance

ABSTRACTSpartina alterniflora (Spartina) is the only halophyte in the salt marsh. However, the molecular basis of its high salt tolerance remains elusive. In this study, we used PacBio full-length single molecule long-read sequencing and RNA-seq to elucidate the transcriptome dynamics of high salt tolerance in Spartina by salt-gradient experiments (0, 350, 500 and 800 mM NaCl). We systematically analyzed the gene expression diversity and deciphered possible roles of ion transporters, protein kinases and photosynthesis in salt tolerance. Moreover, the co-expression network analysis revealed several hub genes in salt stress regulatory networks, including protein kinases such as SaOST1, SaCIPK10 and three SaLRRs. Furthermore, high salt stress affected the gene expression of photosynthesis through down-regulation at the transcription level and alternative splicing at the post-transcriptional level. In addition, overexpression of two Spartina salt-tolerant genes SaHSP70-I and SaAF2 in Arabidopsis significantly promoted the salt tolerance of transgenic lines. Finally, we built the SAPacBio website for visualizing the full-length transcriptome sequences, transcription factors, ncRNAs, salt-tolerant genes, and alternative splicing events in Spartina. Overall, this study sheds light on the high salt tolerance mechanisms of monocotyledonous-halophyte and demonstrates the potential of Spartina genes for engineering salt-tolerant plants.

Purification and characterization of a high salt-tolerant alginate lyase fromCobetiasp. WG-007

2017 ◽  
Vol 64 (4) ◽  
pp. 519-524 ◽  
Author(s):  
Jin-Song Gong ◽  
Xu-Mei Liu ◽  
Ming-Jie Zhang ◽  
Heng Li ◽  
Yan Geng ◽  
...  
Keyword(s):  
Alginate Lyase ◽  
High Salt ◽  
Salt Tolerant ◽  
Purification And Characterization

scholarly journals Studies on genome size estimation, chromosome number, gametophyte development and plant morphology of salt-tolerant halophyte Suaeda salsa

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Yan Cheng ◽  
Pan Yang ◽  
Lihua Zhao ◽  
S. V. G. N. Priyadarshani ◽  
Qiao Zhou ◽  
...  
Keyword(s):  
Genome Size ◽  
Female Gametophyte ◽  
Plant Morphology ◽  
High Salt ◽  
Suaeda Salsa ◽  
Alkali Resistance ◽  
Future Research ◽  
Size Estimation ◽  
Salt Tolerant ◽  
Male And Female

Abstract Background Soil salinization and alkalization are among the major agricultural threats that affect crop productivity worldwide, which are increasing day by day with an alarming rate. In recent years, several halophytes have been investigated for their utilization in soil remediation and to decipher the mechanism of salt-tolerance in these high salt tolerant genetic repositories. Suaeda salsa is an annual halophytic herb in the family Amaranthaceae, displaying high salt and alkali-resistance and having nutritive value. However, the fundamental biological characteristics of this valuable plant remain to be elucidated until today. Results In this study, we observed the morphology and development of Suaeda salsa, including seed morphology, seed germination, plant morphology, and flower development. Using microscopy, we observed the male and female gametophyte developments of Suaeda salsa. Also, chromosome behaviour during the meiosis of male gametophyte was studied. Eventually, the genome size of Suaeda salsa was estimated through flow cytometry using Arabidopsis as reference. Conclusions Our findings suggest that the male and female gametophyte developments of Suaeda salsa are similar to those of the model plant Arabidopsis, and the diploid Suaeda salsa contains nine pairs of chromosomes. The findings also indicate that the haploid genome of Suaeda salsa is approximately 437.5 MB. The observations and results discussed in this study will provide an insight into future research on Suaeda salsa.

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