Fabrication of imidazolium-functionalized magnetic composite microspheres for selective recognition and separation of heme proteins

2017 ◽  
Vol 41 (13) ◽  
pp. 5651-5659 ◽  
Author(s):  
Binghai Wang ◽  
Qian Shao ◽  
Yingtong Fang ◽  
Juanqiang Wang ◽  
Xingjun Xi ◽  
...  

Imidazolium-functionalized magnetic composite microspheres were successfully synthesized, which exhibited high hemoglobin binding capacity (6321 mg g−1) and excellent selectivity.

2017 ◽  
Vol 41 (22) ◽  
pp. 13673-13680 ◽  
Author(s):  
Quan Li ◽  
Binghai Wang ◽  
Juanqiang Wang ◽  
Xingjun Xi ◽  
Qiao Chu ◽  
...  

Thiazolium-functionalized magnetic microspheres, which exhibited an excellent selectivity and a high binding capacity for haemoglobin (5030 mg g−1), were successfully synthesized.


2007 ◽  
Vol 311 (1) ◽  
pp. 145-149 ◽  
Author(s):  
Ben H. Erné ◽  
Maria Claesson ◽  
Stefano Sacanna ◽  
Mark Klokkenburg ◽  
Emile Bakelaar ◽  
...  

1965 ◽  
Vol 11 (5) ◽  
pp. 581-588 ◽  
Author(s):  
C R Valeri ◽  
J C Bond ◽  
K Fowler ◽  
J Sobucki

Abstract A method is described for the quantitation of serum hemoglobin-binding capacity (haptoglobin level) using cellulose acetate membrane electrophoresis. Electrophoresis is carried out for 90 mm. Eight membranes are dried, stained, and scanned within 45 mm. The reproducibility of this technic is within ± 10%.


1979 ◽  
Vol 25 (12) ◽  
pp. 2007-2010 ◽  
Author(s):  
F Van Lente ◽  
A Marchand ◽  
R S Galen

Abstract Serum haptoglobin has been advocated as an indicator of intravascular hemolysis. We have evaluated a nephelometric determination of serum haptoglobin. The assay is sensitive and exhibits within-run precision in the range of 2.5-7.4% coefficient of variation (CV) and between-run precision of 7.0% (CV). In addition, when haptoglobin values determined with the nephelometric assay were compared with hemoglobin-binding capacity determined by electrophoresis, the correlation coefficient was 0.968. The assay is essentially independent of phenotype and free of significant interference by hemolysis. The clinical correlation of haptoglobin values obtained for 100 selected patients with the nephelometric technique correlated well, if less than 250 mg/L, with the presence of hemolytic disease.


2014 ◽  
Vol 926-930 ◽  
pp. 145-149
Author(s):  
Ping Lan ◽  
Yu Xian Feng ◽  
Ri Mei He ◽  
Lei Lei Qiao ◽  
Li Hong Lan ◽  
...  

The magnetic composite microspheres of cassava starch have been prepared by magnetization of one-step oxidation, using cassava starch as natural macromolecule material. The condition effect on magnetic content was evaluated by single factor experiments, such as the mass concentration of starch, reaction temperature, reaction time, stirring speed, initial pH and ferrous solution volume. Preparation conditions were optimized by these evaluation experiments. The prepared magnetic composite microspheres were characterized by infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM), and other means.


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