Remarkable differences in the biochemical fate of Cd2+, Hg2+, CH3Hg+ and thimerosal in red blood cell lysate

Metallomics ◽  
2017 ◽  
Vol 9 (8) ◽  
pp. 1060-1072 ◽  
Author(s):  
Matthew A. Gibson ◽  
Sophia Sarpong-Kumankomah ◽  
Susan Nehzati ◽  
Graham N. George ◽  
Jürgen Gailer
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Lysate ◽  
Toxicological Significance

The application of a metallomics method revealed that all investigated Hg species bound to hemoglobin and that these interactions are of toxicological significance.

Validated hemoglobin-depletion approach for red blood cell lysate proteome analysis by means of 2D PAGE and Orbitrap MS

2012 ◽  
Vol 33 (16) ◽  
pp. 2537-2545 ◽  
Author(s):  
Katja Walpurgis ◽  
Maxie Kohler ◽  
Andreas Thomas ◽  
Folker Wenzel ◽  
Hans Geyer ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Proteome Analysis ◽  
2D Page ◽  
Cell Lysate ◽  
Orbitrap Ms

Enzymatic deconjugation of catecholamines in human and rat plasma and red blood cell lysate

Life Sciences ◽  
1983 ◽  
Vol 33 (10) ◽  
pp. 935-942 ◽  
Author(s):  
Seiichi Yoneda ◽  
Natalie Alexander ◽  
Nicolas D. Vlachakis
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Rat Plasma ◽  
Cell Lysate

scholarly journals Enzymatic hydrolysis of conjugated catecholamines in human and rat plasma and red blood cell lysate

1984 ◽  
Vol 36 ◽  
pp. 308
Author(s):  
Seiichi Yoneda ◽  
Hakuo Takahashi ◽  
Manabu Yoshimura ◽  
Masao Nakagawa ◽  
Natalie Alexander
Keyword(s):  
Enzymatic Hydrolysis ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Rat Plasma ◽  
Cell Lysate ◽  
Hydrolysis Of

Activity Staining in Congenital Factor XIII Deficiency

1979 ◽  
Author(s):  
P. Henriksson ◽  
I.M. Nilsson ◽  
P. Stenberg
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Factor Xiii ◽  
Platelet Rich Plasma ◽  
Staining Procedure ◽  
Factor Xiii Deficiency ◽  
Activity Staining ◽  
B Subunit ◽  
Cell Lysate ◽  
Plasma Factor

Recently, Stenberg and Stenflo [Analytical Biochemistry in press 1979) introduced an activity staining procedure for transamidating enzymes (transglutaminases; endo-γ-glutamine: ε-lysine transferases! e.g. Factor XIII). This specific technique combines agarose gel electrophoresis with activity staining based on the enzyme catalyzed incorporation of mono-dansylthiacadaverine into casein. The method permits detection of plasma Factor XIII activity down to 1% of the normal adult standard. The procedure was applied to plasma and red blood cell lysate from two patients with tentative congenital plasma Factor XIII deficiency (based on clot solubility). No activity was found in platelet poor as well as in platelet rich plasma which confirmed the diagnosis. The red blood cell lysate displayed normal transglutaminase activity which means separate genetic determinations of the plasma and red blod cell trans-amidases. By Immunoelectrophoresis, the plasma Factor XIII b-subunit was quantitated to 43 and 44 % of the concentration in normal standard plasma.

N2Laser-Induced Oxidation of Hemoproteins in Red Blood Cell Lysate

2000 ◽  
Vol 153 (4) ◽  
pp. 411-415
Author(s):  
C. Murali Krishna ◽  
Biplab Bose ◽  
P. K. Gupta
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Lysate
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