A comparative review of computational methods for pathway perturbation analysis: dynamical and topological perspectives

2017 ◽  
Vol 13 (9) ◽  
pp. 1692-1704 ◽  
Author(s):  
Q. Vanhaelen ◽  
A. M. Aliper ◽  
A. Zhavoronkov
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Signaling Pathway ◽  
Computational Methods ◽  
Perturbation Analysis ◽  
Large Scale ◽  
Great Promise ◽  
Environmental Perturbations ◽  
Comparative Review ◽  
Pathway Perturbation

Stem cells offer great promise within the field of regenerative medicine but despite encouraging results, the large scale use of stem cells for therapeutic applications still faces challenges when it comes to controlling signaling pathway responses with respect to environmental perturbations.

scholarly journals Changing Expression Profiles and Inclination to Competing Endogenous RNA Networks on MAPK Signaling Pathways of Human Adipose-Derived Stem Cells in a Direct Current Electric Field

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Mingzhu Jin ◽  
Yujing Zhang ◽  
Yuanyuan Bian ◽  
Ruiqun Qi ◽  
Xinghua Gao
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Signaling Pathway ◽  
Mapk Signaling ◽  
Mapk Signaling Pathway ◽  
Keratinocyte Differentiation ◽  
Adipose Derived Stem Cells ◽  
Competing Endogenous Rna ◽  
Direct Current Electric Field ◽  
Current Electric Field

Adipose-derived stem cells (ADSCs) are an abundant cell source and provide an easy way to harvest mesenchymal stem cells, which are the focus of considerable attention in regenerative medicine. Electric fields (EF) play roles in many biological events and have been reported to promote cell proliferation, migration, and differentiation. In this study, ADSCs were treated with a direct current electric field (DCEF) of either 0 (control group) or 300 mV/mm (EF group) for six hours. RNA screening and analysis revealed that 66, 164, 26, and 1310 circRNAs, lncRNAs, miRNAs, and mRNAs, respectively, were differentially expressed in the DCEF-treated ADSCs compared with untreated ADSCs. Differentially expressed mRNAs were enriched in the MAPK signaling pathway, TNF signaling pathway, and some other pathways. ANXA1, ERRFI1, JAG1, EPHA2, PRR9, and H2AFY2 were related to the keratinocyte differentiation process. Competing endogenous RNA (ceRNA) networks were constructed on the basis of genes in the MAPK signaling pathway. Twenty-one RNAs in the above networks were randomly chosen, and their expression was validated using qRT-PCR, which showed the same expression trends as the RNA sequencing analysis. The MAPK signaling pathway is of great importance in the ADSC processes that occur in a DCEF, including keratinocyte differentiation. Several ceRNAs may participate in the regulation of MAPK signaling. This study may give new insight into the proliferation, migration, and differentiation of ADSCs, which will be valuable for tissue engineering and regenerative medicine.

scholarly journals Designing stem cell niches for differentiation and self-renewal

2018 ◽  
Vol 15 (145) ◽  
pp. 20180388 ◽  
Author(s):  
Hannah Donnelly ◽  
Manuel Salmeron-Sanchez ◽  
Matthew J. Dalby
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Regulatory Networks ◽  
Regulatory Mechanisms ◽  
Great Promise ◽  
Regenerative Capacity ◽  
Cell Niche ◽  
Stem Cell Niches

Mesenchymal stem cells, characterized by their ability to differentiate into skeletal tissues and self-renew, hold great promise for both regenerative medicine and novel therapeutic discovery. However, their regenerative capacity is retained only when in contact with their specialized microenvironment, termed the stem cell niche . Niches provide structural and functional cues that are both biochemical and biophysical, stem cells integrate this complex array of signals with intrinsic regulatory networks to meet physiological demands. Although, some of these regulatory mechanisms remain poorly understood or difficult to harness with traditional culture systems. Biomaterial strategies are being developed that aim to recapitulate stem cell niches, by engineering microenvironments with physiological-like niche properties that aim to elucidate stem cell-regulatory mechanisms, and to harness their regenerative capacity in vitro . In the future, engineered niches will prove important tools for both regenerative medicine and therapeutic discoveries.

Large-Scale Production of Human Adipose Tissue from Stem Cells: A New Tool for Regenerative Medicine and Tissue Banking

2008 ◽  
pp. 110306231138043
Author(s):  
Francesco D'andrea ◽  
Francesco De Francesco ◽  
Giuseppe A. Ferraro ◽  
Vincenzo Desiderio ◽  
Virginia Tirino ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Large Scale ◽  
Tissue Banking ◽  
Human Adipose Tissue ◽  
Scale Production ◽  
Large Scale Production

scholarly journals Safety of Mesenchymal Stem Cells for Clinical Application

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Youwei Wang ◽  
Zhi-bo Han ◽  
Yong-ping Song ◽  
Zhong Chao Han
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Regenerative Medicine ◽  
Therapeutic Agents ◽  
Great Promise ◽  
Safety Issues ◽  
Human Use

Mesenchymal stem cells (MSCs) hold great promise as therapeutic agents in regenerative medicine and autoimmune diseases, based on their differentiation abilities and immunosuppressive properties. However, the therapeutic applications raise a series of questions about the safety of culture-expanded MSCs for human use. This paper summarized recent findings about safety issues of MSCs, in particular their genetic stability in long-termin vitroexpansion, their cryopreservation, banking, and the role of serum in the preparation of MSCs.

scholarly journals Stem Cells: Prospects and Potential Applications in Tanzania: A review

2020 ◽  
Vol 21 (2) ◽  
pp. 1-8
Author(s):  
Afadhali Denis Russa
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Large Scale ◽  
Adult Stem Cells ◽  
Treatment Options ◽  
Stem Cell Technology ◽  
African Countries ◽  
Cell Technology ◽  
Leading Role

Stem cell technology and its application in regenerative medicine is the future gateway for the treatment of most non-communicable diseases (NCDs). As the burden of NCDs continues to rises globally, regenerating the cells, tissues and organs will be the mainstream treatment option. The world is prepared for this intriguing but promising avenue of biomedical technology and medicine but Africa is grossly lagging far behind. African governments, universities, research and health institutions need to take a leading role in empowering and mainstreaming stem cell research.  Moreover, for Africa, there is a huge potential for translating stem cell technology into clinical treatments due to the fact that there are limited treatment options for life-threatening forms of NCDs.  Some African countries have well-developed stem cell facilities and large-scale stem cell therapy centers. The use of adult stem cells in liver failure, diabetes and cardiac infarcts has shown success in some African countries. The present work reviews the status, potential and future prospects of stem cell technology and regenerative medicine in Tanzania with particular emphasis on the adult stem cells applicability into the immediate use inpatient care.  The paper also reviews the available cell identification systems and markers and moral and ethical aspects of stem cell science necessary in the translational treatment regimens. 

scholarly journals Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications

2021 ◽  
Vol 8 (5) ◽  
pp. 68
Author(s):  
Diogo E.S. Nogueira ◽  
Joaquim M.S. Cabral ◽  
Carlos A.V. Rodrigues
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Large Scale ◽  
Adult Stem Cells ◽  
Regulatory Agencies ◽  
Human Stem Cells ◽  
Stem Cell Expansion ◽  
Single Use

Research on human stem cells, such as pluripotent stem cells and mesenchymal stromal cells, has shown much promise in their use for regenerative medicine approaches. However, their use in patients requires large-scale expansion systems while maintaining the quality of the cells. Due to their characteristics, bioreactors have been regarded as ideal platforms to harbour stem cell biomanufacturing at a large scale. Specifically, single-use bioreactors have been recommended by regulatory agencies due to reducing the risk of product contamination, and many different systems have already been developed. This review describes single-use bioreactor platforms which have been used for human stem cell expansion and differentiation, along with their comparison with reusable systems in the development of a stem cell bioprocess for clinical applications.

Rg1 promotes the proliferation and adipogenic differentiation of human adipose-derived stem cells via FXR1/Lnc-GAS5-AS1 pathway

2021 ◽  
Vol 16 ◽  
Author(s):  
Fang-Tian Xu ◽  
Yin-Li Xu ◽  
Yong-Xian Rong ◽  
Dong-Lin Huang ◽  
Zhong-Hong Lai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Signaling Pathway ◽  
Regulatory Network ◽  
Transcriptional Regulatory Network ◽  
Adipogenic Differentiation ◽  
Cell Counting ◽  
Adipose Derived Stem Cells ◽  
Alizarin Red ◽  
Medicine Research

Background: Human adipose-derived stem cells (hASCs) play an important role in regenerative medicine. Objective: Exploring the mechanism of Rg1 in the promotion of the proliferation and adipogenic differentiation of hASCs is important in regenerative medicine research. Methods: In order to observe ginsenoside Rg1 in promoting the proliferation and adipogenic differentiation of hASCs, Rg1 medium at different concentrations was established and tested using the cell counting kit-8 (CCK-8) assay, oil red O staining, alizarin red, and alcian blue. Compared to the control, differentially expressed genes (DEGs) were screened via DEG analysis, which were carried out in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. To explore the relationship among mRNA, long non-coding RNA (lncRNA) and microRNA (miRNA), we constructed a competing endogenous RNA (ceRNA) network. Results: In this study, Rg1 was observed to promote the proliferation and adipogenic differentiation of hASCs. Additionally, enriched BPs and KEGG pathways may be involved in the promotion process, where FXR1 and Lnc-GAS5-AS1 were found to be regulatory factors. The regulatory network suggested that Rg1 could regulate the adipocytokine signaling pathway and IL−17 signaling pathway via FXR1 and Lnc-GAS5-AS1, which served as the mechanism encompassing the promotion of Rg1 on the proliferation and adipogenic differentiation of hASCs. Conclusion: A comprehensive transcriptional regulatory network related to the promotion ability of Rg1 was constructed, revealing mechanisms regarding Rg1’s promotion of the proliferation and adipogenic differentiation of hASCs. The present study provides a theoretical basis in optimizing the function of hASCs.

scholarly journals Large-scale expansion and exploitation of pluripotent stem cells for regenerative medicine purposes: beyond the T flask

2012 ◽  
Vol 7 (1) ◽  
pp. 71-84 ◽  
Author(s):  
Andrew J Want ◽  
Alvin W Nienow ◽  
Christopher J Hewitt ◽  
Karen Coopman
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Pluripotent Stem Cells ◽  
Large Scale ◽  
Scale Expansion

Large-Scale Production of Human Adipose Tissue from Stem Cells: A New Tool for Regenerative Medicine and Tissue Banking

2008 ◽  
Vol 14 (3) ◽  
pp. 233-242 ◽  
Author(s):  
Francesco D'Andrea ◽  
Francesco De Francesco ◽  
Giuseppe A. Ferraro ◽  
Vincenzo Desiderio ◽  
Virginia Tirino ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Large Scale ◽  
Tissue Banking ◽  
Human Adipose Tissue ◽  
Scale Production ◽  
Large Scale Production
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