Amplification-free detection of DNA in a paper-based microfluidic device using electroosmotically balanced isotachophoresis

Lab on a Chip ◽  
2018 ◽  
Vol 18 (6) ◽  
pp. 861-868 ◽  
Author(s):  
Tally Rosenfeld ◽  
Moran Bercovici
Keyword(s):  
Microfluidic Device ◽  
Electroosmotic Flow ◽  
Analytical Device

Microfluidic paper-based analytical device that utilizes the native high electroosmotic flow in nitrocellulose to achieve stationary isotachophoresis focusing.

scholarly journals Electroosmotic flow driven microfluidic device for bacteria isolation using magnetic microbeads

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Samuel Miller ◽  
Alison A. Weiss ◽  
William R. Heineman ◽  
Rupak K. Banerjee
Keyword(s):  
Microfluidic Device ◽  
Electroosmotic Flow ◽  
Point Of Care ◽  
Food Poisoning ◽  
Fluorescent Intensity ◽  
Magnetic Microbeads ◽  
E Coli ◽  
Detection Assay ◽  
Flow Capture ◽  
Improved Performance

Abstract The presence of bacterial pathogens in water can lead to severe complications such as infection and food poisoning. This research proposes a point-of-care electroosmotic flow driven microfluidic device for rapid isolation and detection of E. coli in buffered solution (phosphate buffered saline solution). Fluorescent E. coli bound to magnetic microbeads were driven through the microfluidic device using both constant forward flow and periodic flow switching at concentrations ranging from 2 × 105 to 4 × 107 bacteria/mL. A calibration curve of fluorescent intensity as a function of bacteria concentration was created using both constant and switching flow, showing an increase in captured fluorescent pixel count as concentration increases. In addition, the use of the flow switching resulted in a significant increase in the capture efficiency of E. coli, with capture efficiencies up to 83% ± 8% as compared to the constant flow capture efficiencies (up to 39% ± 11%), with a sample size of 3 µL. These results demonstrate the improved performance associated with the use of the electroosmotic flow switching system in a point-of-care bacterial detection assay.

Determination of nitrite ions in environment analysis with a paper-based microfluidic device

2018 ◽  
Vol 47 (41) ◽  
pp. 14799-14807 ◽  
Author(s):  
Yu-Ci Liu ◽  
Chia-Hui Hsu ◽  
Bing-Jyun Lu ◽  
Peng-Yi Lin ◽  
Mei-Lin Ho
Keyword(s):  
Quantitative Determination ◽  
Microfluidic Device ◽  
Environmental Analysis ◽  
Environment Analysis ◽  
Silver Ink ◽  
Analytical Device

A new microfluidic paper-based analytical device, a (Ag-μPAD)-based chemiresistor composed of silver ink, has been developed for the selective, sensitive, and quantitative determination of nitrite ions in environmental analysis.

Novel Microfluidic Device Integrated with a Fluidic-Capacitor to Mimic Heart Beating for Generation of Functional Liver Organoids

2019 ◽  
Vol 139 (7) ◽  
pp. 209-216
Author(s):  
Jiaxu Wu ◽  
Yoshikazu Hirai ◽  
Ken-ichiro Kamei ◽  
Toshiyuki Tsuchiya ◽  
Osamu Tabata
Keyword(s):  
Microfluidic Device ◽  
Heart Beating ◽  
Liver Organoids

Pneumatically-driven Microfluidic Device for Evaluating Active Transport by Kinesin Motor Protein

2016 ◽  
Vol 136 (9) ◽  
pp. 384-389
Author(s):  
Kazuya Fujimoto ◽  
Hirofumi Shintaku ◽  
Hidetoshi Kotera ◽  
Ryuji Yokokawa
Keyword(s):  
Active Transport ◽  
Microfluidic Device ◽  
Motor Protein ◽  
Kinesin Motor

A microfluidic device with integrated impedance detection for λ-DNA

2003 ◽  
Vol 773 ◽  
Author(s):  
Myung-Il Park ◽  
Jonging Hong ◽  
Dae Sung Yoon ◽  
Chong-Ook Park ◽  
Geunbae Im
Keyword(s):  
Microfluidic Device ◽  
Electrochemical Impedance ◽  
Direct Detection ◽  
Full Potential ◽  
Label Free ◽  
Buffer Solution ◽  
Detection Systems ◽  
Significant Difference ◽  
Detection Of Biomolecules ◽  
Impedance Magnitude

AbstractThe large optical detection systems that are typically utilized at present may not be able to reach their full potential as portable analysis tools. Accurate, early, and fast diagnosis for many diseases requires the direct detection of biomolecules such as DNA, proteins, and cells. In this research, a glass microchip with integrated microelectrodes has been fabricated, and the performance of electrochemical impedance detection was investigated for the biomolecules. We have used label-free λ-DNA as a sample biomolecule. By changing the distance between microelectrodes, the significant difference between DW and the TE buffer solution is obtained from the impedance-frequency measurements. In addition, the comparison for the impedance magnitude of DW, the TE buffer, and λ-DNA at the same distance was analyzed.

A Microfluidic Device for Studying Mass Transfer Effects in Biomolecular Analysis

2002 ◽  
Author(s):  
Min Yue ◽  
Katherine Dunphy ◽  
Jerry Jenkins ◽  
Christopher Dames ◽  
Guanghua Wu ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Microfluidic Device ◽  
Transfer Effects ◽  
Biomolecular Analysis
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