Integrated dual-modality microfluidic sensor for biomarker detection using lithographic plasmonic crystal

Lab on a Chip ◽  
2018 ◽  
Vol 18 (5) ◽  
pp. 803-817 ◽  
Author(s):  
Md. Azahar Ali ◽  
Shawana Tabassum ◽  
Qiugu Wang ◽  
Yifei Wang ◽  
Ratnesh Kumar ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Sample Volume ◽  
Periodic Array ◽  
Sensor Chip ◽  
Biomarker Detection ◽  
Limited Sample ◽  
Dual Modality ◽  
Plasmonic Crystal ◽  
Biomarker Molecules

This paper reports an integrated dual-modality microfluidic sensor chip, consisting of a patterned periodic array of nanoposts coated with gold (Au) and graphene oxide (GO), to detect target biomarker molecules in a limited sample volume.

Carboxylic group riched graphene oxide based disposable electrochemical immunosensor for cancer biomarker detection

2018 ◽  
Vol 545 ◽  
pp. 13-19 ◽  
Author(s):  
Sajid Rauf ◽  
Geetesh K. Mishra ◽  
Jahanzaib Azhar ◽  
Rupesh K. Mishra ◽  
K. Yugender Goud ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Cancer Biomarker ◽  
Electrochemical Immunosensor ◽  
Biomarker Detection ◽  
Carboxylic Group

scholarly journals Alternative Method for HDL and Exosome Isolation with Small Serum Volumes and Their Characterizations

Separations ◽  
2021 ◽  
Vol 8 (11) ◽  
pp. 204
Author(s):  
Rian Ka Praja ◽  
Wisitsak Phoksawat ◽  
Patcharaporn Tippayawat ◽  
Amonrat Jumnainsong ◽  
Chanvit Leelayuwat
Keyword(s):  
High Density Lipoprotein ◽  
Protein Pattern ◽  
Density Lipoprotein ◽  
Sample Volume ◽  
Molecular Diagnostic ◽  
Particle Sizes ◽  
Limited Sample ◽  
Modified Method ◽  
Diagnostic Approaches ◽  
Commercial Kits

High-density lipoprotein (HDL) and exosomes are promising sources of biomarkers. However, the limited sample volume and access to the ultracentrifuge equipment are still an issue during HDL and exosome isolation. This study aimed to isolate HDL and exosomes using an ultracentrifugation-free method with various small serum volumes. HDL was isolated from 200 µL (HDL200) and 500 µL (HDL500) of sera. Three different volumes: 50 µL (Exo50), 100 µL (Exo100), and 250 µL (Exo250) were used for exosome isolation. HDL and exosomes were isolated using commercial kits with the modified method and characterized by multiple approaches. The HDL levels of HDL200 and HDL500 were not significantly different (p > 0.05), with percent recoveries of >90%. HDL200 and HDL500 had the same protein pattern with a biochemical similarity of 99.60 ± 0.10%. The particle sizes of Exo50, Exo100, and Exo250 were in the expected range. All isolated exosomes exhibited a similar protein pattern with a biochemical similarity of >99%. In conclusion, two different serum volumes (200 and 500 µL) and three different serum volumes (50, 100, and 250 µL) can be employed for HDL and exosome isolation, respectively. The possibility of HDL and exosome isolation with small volumes will accelerate biomarker discoveries with various molecular diagnostic approaches.

scholarly journals An electrochemical biosensor for prostate cancer biomarker detection using graphene oxide-gold nanostructures

2019 ◽  
Vol 19 (3) ◽  
pp. 206-216 ◽  
Author(s):  
Zahra Akbari jonous ◽  
Javad Shabani Shayeh ◽  
Fatemeh Yazdian ◽  
Amir Yadegari ◽  
Mohadeseh Hashemi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Graphene Oxide ◽  
Electrochemical Biosensor ◽  
Cancer Biomarker ◽  
Gold Nanostructures ◽  
Biomarker Detection

scholarly journals Graphene oxide–metal nanocomposites for cancer biomarker detection

RSC Advances ◽  
2017 ◽  
Vol 7 (57) ◽  
pp. 35982-35991 ◽  
Author(s):  
Md. Azahar Ali ◽  
Chandan Singh ◽  
Saurabh Srivastava ◽  
Prasad Admane ◽  
Ved V. Agrawal ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Graphene Oxide ◽  
Black Pepper ◽  
Cancer Biomarker ◽  
Biomarker Detection ◽  
Reduced Graphene ◽  
Metal Nanocomposites ◽  
Universal Protocol ◽  
Graphene Oxide Sheets

We report a universal protocol for the in situ bioinspired green synthesis of metal nanoparticles on simultaneously reduced graphene oxide sheets using a black pepper extract for quantification of breast cancer biomarker.

scholarly journals Microfluidic Overhauser DNP Chip for Signal-enhanced Compact NMR

2020 ◽  
Author(s):  
Sebastian Kiss ◽  
Neil MacKinnon ◽  
Jan Korvink
Keyword(s):  
Point Of Care ◽  
Power Level ◽  
Sample Volume ◽  
Spectroscopic Technique ◽  
Small Sample ◽  
Biomarker Detection ◽  
Low Field ◽  
Equilibrium Level ◽  
Microwave Power Level

Abstract Nuclear magnetic resonance at low field strength is an insensitive spectroscopic technique, precluding portable applications with small sample volumes, such as needed for biomarker detection in body fluids. Here we report a compact double resonant chip stack system that implements in situ dynamic nuclear polarisation of a 130 nL sample volume, achieving a signal enhancement of 54 w.r.t. the thermal equilibrium level at a microwave power level of 0.5W. This work overcomes instrumental barriers to the use of NMR detection for point-of-care applications.

scholarly journals Time to Detection of Growth for Mycobacterium tuberculosis in a Low Incidence Area

2021 ◽  
Vol 11 ◽  
Author(s):  
Rechana Vongthilath-Moeung ◽  
Antoine Poncet ◽  
Gesuele Renzi ◽  
Jacques Schrenzel ◽  
Jean-Paul Janssens
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Lung Disease ◽  
Clinical Management ◽  
Sample Volume ◽  
University Hospital ◽  
Thoracic Disease ◽  
Limited Sample ◽  
Time To Detection ◽  
Low Incidence ◽  
Culture Positive

BackgroundDiagnosis of Mycobacterium tuberculosis (MTB) infection can be confirmed by Xpert assays within hours. However, when sample size does not allow performing both culture and Xpert, or if Xpert is negative, then formal diagnosis of MTB relies on culture and time to detection of growth (TDG) becomes critical for clinical management.ObjectivesTo determine TDG in Xpert negative samples, or in samples in which Xpert could not be performed, in a low-incidence area for MTB.MethodsRetrospective analysis (2015-2020) of a database including all cultures for mycobacteria in a University Hospital covering approximately 500’000 inhabitants. Analysis was restricted to culture positive (C+) samples for MTB for which 1/Xpert was negative or could not be performed because of limited sample volume, and 2/collected from subjects treated less than 24 hours. TDG was analyzed according to microscopy, origin of sample (pulmonary or not) and presence of cavitation.ResultsAmong 837 C+ samples for MTB, 236 samples (80% of respiratory origin) from 147 patients fulfilled study criteria; 78 samples (49 patients, 33%) were acid-fast bacilli (AFB) positive. Median (IQR) TDG was 25 (17; 40) days for all samples. TDG exceeded 28 days in 43% of samples and was significantly shorter in AFB+ vs AFB- samples, and samples from cavitary vs non cavitary or extra-thoracic disease.ConclusionsIn Xpert negative samples, or samples for which Xpert could not be performed, TDG exceeded 4 weeks in 43% of samples. AFB+ and samples from cavitary lung disease had a significantly shorter TDG.

scholarly journals Bioinspired Synergy Sensor Chip of Photonic Crystals-Graphene Oxide for Multiamines Recognition

2018 ◽  
Vol 90 (11) ◽  
pp. 6371-6375 ◽  
Author(s):  
Wanjie Ren ◽  
Meng Qin ◽  
Xiaotian Hu ◽  
Fengyu Li ◽  
Yuanfeng Wang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Photonic Crystals ◽  
Sensor Chip
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