Isotachophoresis applied to biomolecular reactions

Lab on a Chip ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 11-26 ◽  
Author(s):  
C. Eid ◽  
J. G. Santiago
Keyword(s):  
Nucleic Acids ◽  
Chemical Reactions ◽  
Live Cells ◽  
Biomolecular Reactions

This review discusses research developments and applications of isotachophoresis (ITP) to the initiation, control, and acceleration of chemical reactions, emphasizing reactions involving biomolecular reactants such as nucleic acids, proteins, and live cells.

scholarly journals Visible Light Photochemical Reactions for Nucleic Acid-Based Technologies

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 556
Author(s):  
Bonwoo Koo ◽  
Haneul Yoo ◽  
Ho Jeong Choi ◽  
Min Kim ◽  
Cheoljae Kim ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Visible Light ◽  
Chemical Reactions ◽  
Chemical Biology ◽  
Photochemical Reactions ◽  
Reaction Conditions ◽  
Promising Tool ◽  
Recent Developments ◽  
Visible Light Driven

The expanding scope of chemical reactions applied to nucleic acids has diversified the design of nucleic acid-based technologies that are essential to medicinal chemistry and chemical biology. Among chemical reactions, visible light photochemical reaction is considered a promising tool that can be used for the manipulations of nucleic acids owing to its advantages, such as mild reaction conditions and ease of the reaction process. Of late, inspired by the development of visible light-absorbing molecules and photocatalysts, visible light-driven photochemical reactions have been used to conduct various molecular manipulations, such as the cleavage or ligation of nucleic acids and other molecules as well as the synthesis of functional molecules. In this review, we describe the recent developments (from 2010) in visible light photochemical reactions involving nucleic acids and their applications in the design of nucleic acid-based technologies including DNA photocleaving, DNA photoligation, nucleic acid sensors, the release of functional molecules, and DNA-encoded libraries.

Analyzing the structure of macromolecules in their native cellular environment using hydroxyl radical footprinting

The Analyst ◽  
2018 ◽  
Vol 143 (4) ◽  
pp. 798-807 ◽  
Author(s):  
Emily E. Chea ◽  
Lisa M. Jones
Keyword(s):  
Nucleic Acids ◽  
Hydroxyl Radical ◽  
Live Cells ◽  
Cellular Environment ◽  
Hydroxyl Radical Footprinting

Hydroxyl radical footprinting (HRF) has been successfully used to study the structure of both nucleic acids and proteins in live cells.

scholarly journals Enantiomer excesses of rare and common sugar derivatives in carbonaceous meteorites

2016 ◽  
Vol 113 (24) ◽  
pp. E3322-E3331 ◽  
Author(s):  
George Cooper ◽  
Andro C. Rios
Keyword(s):  
Nucleic Acids ◽  
Organic Compounds ◽  
Chemical Reactions ◽  
Prebiotic Chemistry ◽  
Physical Processes ◽  
Sugar Alcohols ◽  
Racemic Mixtures ◽  
Biological Polymers ◽  
Sugar Derivatives ◽  
Enantiomer Composition

Biological polymers such as nucleic acids and proteins are constructed of only one—the d or l—of the two possible nonsuperimposable mirror images (enantiomers) of selected organic compounds. However, before the advent of life, it is generally assumed that chemical reactions produced 50:50 (racemic) mixtures of enantiomers, as evidenced by common abiotic laboratory syntheses. Carbonaceous meteorites contain clues to prebiotic chemistry because they preserve a record of some of the Solar System’s earliest (∼4.5 Gy) chemical and physical processes. In multiple carbonaceous meteorites, we show that both rare and common sugar monoacids (aldonic acids) contain significant excesses of the d enantiomer, whereas other (comparable) sugar acids and sugar alcohols are racemic. Although the proposed origins of such excesses are still tentative, the findings imply that meteoritic compounds and/or the processes that operated on meteoritic precursors may have played an ancient role in the enantiomer composition of life’s carbohydrate-related biopolymers.

scholarly journals Determining the Quantitative Dynamics of Nucleic Acids in Live Cells through Rics and iMSD Approaches

2014 ◽  
Vol 106 (2) ◽  
pp. 67a
Author(s):  
Stephen P. Mieruszynski ◽  
Michelle A. Digman ◽  
Enrico Gratton ◽  
Mark Jones
Keyword(s):  
Nucleic Acids ◽  
Live Cells

scholarly journals Chemical Synthesis of Stimuli-Responsive Guide RNA for Conditional Control of CRISPR-Cas9 Gene Editing

2021 ◽  
Author(s):  
Chunmei Gu ◽  
Lu Xiao ◽  
Jiachen Shang ◽  
Xiao Xu ◽  
Luo He ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Nucleic Acids ◽  
Gene Editing ◽  
Live Cells ◽  
Stimuli Responsive ◽  
Guide Rna ◽  
Conditional Control ◽  
Target Effects

CRISPR-Cas9 promotes changes in identity or abundance of nucleic acids in live cells and is a programmable modality of broad biotechnological and therapeutic interest. To reduce off-target effects, tools for...

scholarly journals Mapping life's reactions: A brief history of metabolic pathways and their regulation

2009 ◽  
Vol 31 (3) ◽  
pp. 4-7
Author(s):  
Philip Newsholme
Keyword(s):  
Nucleic Acids ◽  
Chemical Reactions ◽  
Chemical Reaction ◽  
Metabolic Pathway ◽  
Metabolic Pathways ◽  
Living Cell ◽  
Complete Synthesis ◽  
History Of ◽  
A Chain ◽  
Almost All

It is now recognized that almost all chemical reactions that take place in a living cell require an enzyme to catalyse the reaction. An enzyme catalyses one (or, in rare cases, more than one closely related) chemical reaction. The complete synthesis or degradation of complex biological substances such as glycogen, nucleic acids, proteins and lipids requires a series of linked sequences of reactions. A chain of such reactions is referred to as a ‘metabolic pathway’.

A Monomethine Cyanine Dye Cyan 40 for Two-photon-excited Fluorescence Detection of Nucleic Acids and Their Visualization in Live Cells¶

2007 ◽  
Vol 77 (2) ◽  
pp. 138-145 ◽  
Author(s):  
Tymish Y. Ohulchanskyy ◽  
Haridas E. Pudavar ◽  
Sergiy M. Yarmoluk ◽  
Valeriy M. Yashchuk ◽  
Earl J. Bergey ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Fluorescence Detection ◽  
Cyanine Dye ◽  
Live Cells ◽  
Two Photon
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