On-chip cell sorting by high-speed local-flow control using dual membrane pumps

Lab on a Chip ◽  
2017 ◽  
Vol 17 (16) ◽  
pp. 2760-2767 ◽  
Author(s):  
Shinya Sakuma ◽  
Yusuke Kasai ◽  
Takeshi Hayakawa ◽  
Fumihito Arai
Keyword(s):  
Cell Viability ◽  
Success Rate ◽  
High Throughput ◽  
Microfluidic Chip ◽  
Euglena Gracilis ◽  
Cell Sorting ◽  
High Speed ◽  
Local Flow ◽  
On Chip ◽  
Membrane Pumps

We achieved high-throughput on-chip sorting of large cells by using on-chip dual membrane pumps integrated with a high-rigidity microfluidic chip. In the case of Euglena gracilis sorting, the throughput was 23 kHz with 92.8% success rate, 95.8% purity, and 90.8% cell viability.

Ultra-high-speed on-chip cell sorting by using local flow control

2017 ◽  
Vol 2017 (0) ◽  
pp. 2P1-P06
Author(s):  
Yusuke KASAI ◽  
Shinya SAKUMA ◽  
Takeshi HAYAKAWA ◽  
Fumihito ARAI
Keyword(s):  
Flow Control ◽  
Cell Sorting ◽  
High Speed ◽  
Local Flow ◽  
Ultra High Speed ◽  
On Chip

scholarly journals High-throughput microfluidic cell sorting platform (MICS)

2019 ◽  
Author(s):  
David Philpott ◽  
Peter Aldridge ◽  
Barbara Mair ◽  
Randy Atwal ◽  
Sanna Masud ◽  
...  
Keyword(s):  
Protein Expression ◽  
Cell Viability ◽  
High Throughput ◽  
Cell Sorting ◽  
Mammalian Cells ◽  
Large Scale ◽  
Genetic Screens ◽  
Large Numbers ◽  
Conventional Cell ◽  
Set Up

Abstract Genome-scale functional genetic screens can be used to interrogate determinants of protein expression modulation of a target of interest. Such phenotypic screening approaches typically require sorting of large numbers of cells (>108). In conventional cell sorting techniques (i.e. fluorescence-activated cell sorting), sorting time, associated with high instrument and operating costs and loss of cell viability, are limiting to the scalability and throughput of these screens. We recently established a rapid and scalable high-throughput microfluidic cell sorting platform (MICS) using immunomagnetic nanoparticles to sort cells in parallel capable of sorting more than 108 HAP1 cells in under one hour while maintaining high levels of cell viability (Ref. 1). This protocol outlines how to set-up MICS for large-scale phenotypic screens in mammalian cells. We anticipate this platform being used for genome-wide functional genetic screens as well as other applications requiring the sorting of large numbers of cells based on protein expression.

scholarly journals Scalable, FACS-Free Genome-Wide Phenotypic Screening

2019 ◽  
Author(s):  
Barbara Mair ◽  
Peter M. Aldridge ◽  
Randy S. Atwal ◽  
Sanna N. Masud ◽  
Meng Zhang ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cell Viability ◽  
High Throughput ◽  
Cell Sorting ◽  
Phenotypic Screening ◽  
Genetic Screens ◽  
Entire Genome ◽  
Genome Wide ◽  
N Terminus ◽  
Genome Scale

ABSTRACTGenome-scale functional genetic screens can identify key regulators of a phenotype of interest, such as determinants of protein expression or modification. Here, we present a rapid, high-throughput approach to phenotypic CRISPR-Cas9 screening. To study factors that modulate the display of CD47 on the cell surface, we processed an entire genome-wide screen containing more than 108cells in under one hour and maintained high levels of cell viability using a highly scalable cell sorting technology. We robustly identified modulators of CD47 function including QPCTL, an enzyme required for formation of the pyroglutamyl modification at the N-terminus of this protein.

scholarly journals High-speed On-chip Cell Sorting

2019 ◽  
Vol 59 (5) ◽  
pp. 248-254
Author(s):  
Shinya SAKUMA ◽  
Yusuke KASAI ◽  
Fumihito ARAI
Keyword(s):  
Cell Sorting ◽  
High Speed ◽  
On Chip

scholarly journals Continuous-flow microfluidic blood cell sorting for unprocessed whole blood using surface-micromachined microfiltration membranes

Lab on a Chip ◽  
2014 ◽  
Vol 14 (14) ◽  
pp. 2565-2575 ◽  
Author(s):  
Xiang Li ◽  
Weiqiang Chen ◽  
Guangyu Liu ◽  
Wei Lu ◽  
Jianping Fu
Keyword(s):  
Blood Cell ◽  
High Throughput ◽  
Microfluidic Chip ◽  
Cell Sorting ◽  
Whole Blood ◽  
Continuous Flow ◽  
Microfiltration Membranes

A microfluidic chip for continuous-flow isolation of WBCs from unprocessed whole blood with high throughput and purity.

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