An automated and portable microfluidic chemiluminescence immunoassay for quantitative detection of biomarkers

Lab on a Chip ◽  
2017 ◽  
Vol 17 (13) ◽  
pp. 2225-2234 ◽  
Author(s):  
Binfeng Hu ◽  
Juanjuan Li ◽  
Lei Mou ◽  
Yong Liu ◽  
Jinqi Deng ◽  
...  
Keyword(s):  
Quantitative Detection ◽  
Serum Samples ◽  
Chemiluminescence Immunoassay ◽  
Detection Of Biomarkers

An automated and portable microfluidic chemiluminescence immunoassay is developed for quantitative detection of biomarkers in serum samples.

scholarly journals An automated microfluidic chemiluminescence immunoassay platform for quantitative detection of biomarkers

2018 ◽  
Vol 20 (4) ◽  
Author(s):  
Xiaoping Min ◽  
Da Fu ◽  
Jianzhong Zhang ◽  
Juntian Zeng ◽  
Zhenyu Weng ◽  
...  
Keyword(s):  
Quantitative Detection ◽  
Chemiluminescence Immunoassay ◽  
Detection Of Biomarkers

scholarly journals Diagnostic Significance of Serum Galectin-3 in Hospitalized Patients with COVID-19—A Preliminary Study

Biomolecules ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1136
Author(s):  
Beata Kuśnierz-Cabala ◽  
Barbara Maziarz ◽  
Paulina Dumnicka ◽  
Marcin Dembiński ◽  
Maria Kapusta ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Hospital Stay ◽  
Tissue Injury ◽  
Immunological Response ◽  
Organ Injury ◽  
Quantitative Detection ◽  
Serum Samples ◽  
Diagnostic Significance ◽  
Galectin 3 ◽  
Preliminary Study

Severe coronavirus disease 2019 (COVID-19) is associated with hyperinflammation leading to organ injury, including respiratory failure. Galectin-3 was implicated in innate immunological response to infections and in chronic fibrosis. The aim of our preliminary study was the assessment of the diagnostic utility of serum galectin-3 in patients with COVID-19. The prospective observational study included adult patients admitted with active COVID-19 and treated in tertiary hospital between June and July 2020. The diagnosis was confirmed by the quantitative detection of nucleic acid of severe acute respiratory syndrome coronavirus 2 in nasopharyngeal swabs. Galectin-3 was measured by enzyme immunoassay in serum samples obtained during the first five days of hospital stay. We included 70 patients aged 25 to 73 years; 90% had at least one comorbidity. During the hospital stay, 32.9% were diagnosed with COVID-19 pneumonia and 12.9% required treatment in the intensive care unit (ICU). Serum galectin-3 was significantly increased in patients who developed pneumonia, particularly those who required ICU admission. Positive correlations were found between galectin-3 and inflammatory markers (interleukin-6, C-reactive protein, ferritin, pentraxin-3), a marker of endothelial injury (soluble fms-like tyrosine kinase-1), and a range of tissue injury markers. Serum galectin-3 enabled the diagnosis of pneumonia with moderate diagnostic accuracy and the need for ICU treatment with high diagnostic accuracy. Our findings strengthen the hypothesis that galectin-3 may be involved in severe COVID-19. Further studies are planned to confirm the preliminary results and to verify possible associations of galectin-3 with long-term consequences of COVID-19, including pulmonary fibrosis.

scholarly journals Development of a Smartphone-Based Nanozyme-Linked Immunosorbent Assay for Quantitative Detection of SARS-CoV-2 Nucleocapsid Phosphoprotein in Blood

2021 ◽  
Vol 12 ◽  
Author(s):  
Bochao Liu ◽  
Ze Wu ◽  
Chaolan Liang ◽  
Jinhui Lu ◽  
Jinfeng Li ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Cross Reactivity ◽  
Quantitative Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Primary Screening ◽  
Global Pandemic ◽  
Novel Coronavirus ◽  
Acid Test ◽  
Control Samples

Since December 2019, a novel coronavirus (SARS-CoV-2) has resulted in a global pandemic of coronavirus disease (COVID-19). Although viral nucleic acid test (NAT) has been applied predominantly to detect SARS-CoV-2 RNA for confirmation diagnosis of COVID-19, an urgent need for alternative, rapid, and sensitive immunoassays is required for primary screening of virus. In this study, we developed a smartphone-based nanozyme-linked immunosorbent assay (SP-NLISA) for detecting the specific nucleocapsid phosphoprotein (NP) of SARS-CoV-2 in 37 serum samples from 20 COVID-19 patients who were diagnosed by NAT previously. By using SP-NLISA, 28/37 (75.7%) serum samples were detected for NP antigens and no cross-reactivity with blood donors’ control samples collected from different areas of China. In a control assay using the conventional enzyme-linked immunosorbent assay (ELISA), only 7/37 (18.91%) serum samples were detected for NP antigens and no cross-reactivity with control samples. SP-NLISA could be used for rapid detection of SARS-CoV-2 NP antigen in primary screening of SARS-CoV-2 infected individuals.

scholarly journals Establishment of a Screening Method for Epstein-Barr Virus-Associated Gastric Carcinoma by Droplet Digital PCR

2019 ◽  
Vol 7 (12) ◽  
pp. 628 ◽  
Author(s):  
Takuya Shuto ◽  
Jun Nishikawa ◽  
Kanami Shimokuri ◽  
Ayaka Yanagi ◽  
Tatsuya Takagi ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gastric Carcinoma ◽  
Epstein Barr Virus ◽  
Screening Method ◽  
Complete Agreement ◽  
Quantitative Detection ◽  
Serum Samples ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

Background: Epstein-Barr virus-associated gastric carcinoma (EBVaGC) is classified as one of the molecular subtypes of gastric cancer. We used droplet digital polymerase chain reaction (ddPCR) to enable highly sensitive and quantitative detection of EBV. Methods: EBV-DNA load was calculated based on the copy number of the BamH1-W fragment of EBV by ddPCR, and the cut-off value of EBV-DNA load was set. We conducted both ddPCR and EBER1 ISH to examine whether their results coincided in 158 gastric cancer specimens of unknown EBV status. We prepared 26 biopsy specimens and 49 serum samples including EBVaGC and assayed them by ddPCR. Results: The median values of EBV-DNA load for EBVaGC and EBV-negative control were 17.0 and 0.00308, respectively. A cut-off value of 0.032 was determined for which the sensitivity was 1. Among the 158 gastric cancer specimens, 14 lesions were judged as EBV-positive by the 0.032 cut-off value determined by ddPCR. The results of ddPCR and EBER1 ISH were in complete agreement. Even when using a biopsy specimen as a sample for ddPCR, the EBV-DNA load of all EBVaGCs was larger than the cut-off value. Conclusions: We established a new method of diagnosing EBVaGC from tissue samples by ddPCR.

scholarly journals Quantum dot nanobead-based immunochromatographic assay for the quantitative detection of the procalcitonin antigen in serum samples

2020 ◽  
Vol 159 ◽  
pp. 105533
Author(s):  
Shu Zhou ◽  
Yuanli Peng ◽  
Jing Hu ◽  
Hong Duan ◽  
Tongtong Ma ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Serum Samples

Synthesis of novel cationic carbon dots and application to quantitative detection of K+ in human serum samples

2019 ◽  
Vol 43 (46) ◽  
pp. 17937-17940 ◽  
Author(s):  
Zideng Gao ◽  
Shunyi Wang ◽  
Zijun Xu ◽  
Jin Liu ◽  
Yuanfang Huang ◽  
...  
Keyword(s):  
Human Serum ◽  
Carbon Dots ◽  
High Sensitivity ◽  
Quantitative Detection ◽  
Serum Samples ◽  
Human Serum Samples

Novel cationic carbon dots were synthesized in a simple way and applied to detect K+ in human serum samples with ultra-high sensitivity.

scholarly journals Detection of C-Reactive Protein Using Histag-HRP Functionalized Nanoconjugate with Signal Amplified Immunoassay

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1240
Author(s):  
Mohd Farhan Siddiqui ◽  
Zeeshan Ahmad Khan ◽  
Seungkyung Park
Keyword(s):  
Detection Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
C Reactive Protein ◽  
Serum Samples ◽  
Reactive Protein ◽  
Elisa Format ◽  
Early Biomarker ◽  
Cost Efficient ◽  
Detection Of Biomarkers ◽  
Gold Nanoprobe

Ultrasensitive detection of biomarkers is highly significant for disease prognosis and public health treatment. Despite wide acceptance in routine laboratory tests, the conventional enzyme-linked immunosorbent assay (ELISA) has been of limited use for early biomarker detection due to insufficient sensitivity and multiple long incubation time. Several nanoprobes have been introduced to circumvent the limitation, however, rapid, simple, and chemical-free nanoprobe synthesis and sensitive detection methods, particularly for ELISA, are still lacking. In this study, we have synthesized a gold nanoprobe, conjugated with multiple 6X-histidine (6X-his) peptide and nickel-horseradish peroxidase (Ni2+-HRP), for enhancing the colorimetric signal in ELISA. The developed nanoprobe has been tested for the detection of immunologically significant C-reactive protein (CRP) in ELISA format. The performance of designed probe is validated by testing standard and serum samples, and the detection limit of 32.0 pg/mL with R2 = 0.98 is confirmed. Furthermore, a comparative analysis of the developed nanoprobe was performed with ELISA developed on conventional guidelines, the proposed immunoassay showed an increase of 12-fold sensitivity for detecting CRP due to the high loading of 6Xhis peptide and binding of multiple Ni2+-HRP on a gold nanoparticle. Additionally, the proposed assay provides a simple, fast, and cost-efficient (not requiring multiple antibodies) detection of CRP with easy nanoprobe synthesis. Moreover, the developed Histag-HRP functionalized nanoconjugate immunoassay is flexible and can be applied to other biomarkers efficiently by using disease specific antibody.

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