Total reflection X-ray fluorescence based quantification of gold nanoparticles in cancer cells

2018 ◽  
Vol 33 (3) ◽  
pp. 395-403 ◽  
Author(s):  
Gabriella Mankovskii ◽  
Ana Pejović-Milić
Keyword(s):  
Gold Nanoparticles ◽  
Sample Preparation ◽  
Cancer Cells ◽  
Sample Volume ◽  
Total Reflection ◽  
X Ray ◽  
Minimal Sample

A small (5 μl) sample volume and minimal sample preparation steps are required to accurately quantify AuNP uptake in cancer cells.

scholarly journals Quantification of gold nanoparticles in biological samples with total reflection x-ray fluoresence

2021 ◽  
Author(s):  
Gabriella Mankovskii
Keyword(s):  
Gold Nanoparticles ◽  
Sample Preparation ◽  
Internal Standard ◽  
Cancer Cell Line ◽  
Organic Matrix ◽  
Total Reflection ◽  
Nanoparticle Uptake ◽  
Preparation Methods ◽  
X Ray ◽  
Inductively Coupled

The applications of gold nanoparticles (AuNPs) in biomedicine span the imaging, diagnosis and therapy domains. Current methods of nanoparticle quantification are based on inductively coupled plasmas (ICP) methods, which are heavily influenced by sample preparation. This becomes challenging when quantifying trace-level amount of AuNPs in the presence of organic matrix. In this thesis, total reflection X-ray fluorescence (TXRF) spectrometry is proposed for quantifying cellular uptake of AuNPs in MDA-MB-231 breast cancer cell line suspensions. The most suitable internal standard and fitting approach that yield 90-110% recovery rate along with different sample preparation methods were investigated. Reference material AuNPs were used to validate the quantification capabilities of the newly developed method. Direct comparison of TXRF and ICP-AES showed ability of TXRF to accurately quantify nanoparticle uptake while further demonstrating the importance of sample preparation for ICP-AES. These results suggest that TXRF has potential for quantifying nanoparticle uptake and their kinetics.

scholarly journals Quantification of gold nanoparticles in biological samples with total reflection x-ray fluoresence

2021 ◽  
Author(s):  
Gabriella Mankovskii
Keyword(s):  
Gold Nanoparticles ◽  
Sample Preparation ◽  
Internal Standard ◽  
Cancer Cell Line ◽  
Organic Matrix ◽  
Total Reflection ◽  
Nanoparticle Uptake ◽  
Preparation Methods ◽  
X Ray ◽  
Inductively Coupled

The applications of gold nanoparticles (AuNPs) in biomedicine span the imaging, diagnosis and therapy domains. Current methods of nanoparticle quantification are based on inductively coupled plasmas (ICP) methods, which are heavily influenced by sample preparation. This becomes challenging when quantifying trace-level amount of AuNPs in the presence of organic matrix. In this thesis, total reflection X-ray fluorescence (TXRF) spectrometry is proposed for quantifying cellular uptake of AuNPs in MDA-MB-231 breast cancer cell line suspensions. The most suitable internal standard and fitting approach that yield 90-110% recovery rate along with different sample preparation methods were investigated. Reference material AuNPs were used to validate the quantification capabilities of the newly developed method. Direct comparison of TXRF and ICP-AES showed ability of TXRF to accurately quantify nanoparticle uptake while further demonstrating the importance of sample preparation for ICP-AES. These results suggest that TXRF has potential for quantifying nanoparticle uptake and their kinetics.

Release kinetics of gold nanoparticles from collagen microcapsules by total reflection X-ray fluorescence

2013 ◽  
Vol 417 ◽  
pp. 83-88 ◽  
Author(s):  
Svetlana Erokhina ◽  
Oleg Konovalov ◽  
Paolo Bianchini ◽  
Alberto Diaspro ◽  
Carmelina Ruggiero ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Release Kinetics ◽  
Total Reflection ◽  
X Ray ◽  
Kinetics Of

A green analytical method for the determination of Cu, Fe, Mn, and Zn in wheat flour using total reflection X-ray fluorescence

2018 ◽  
Vol 33 (7) ◽  
pp. 1264-1268 ◽  
Author(s):  
Ignacio Machado ◽  
Sebastián Mondutey ◽  
Natalia Pastorino ◽  
Valeria Arce ◽  
Mariela Pistón
Keyword(s):  
Sample Preparation ◽  
Wheat Flour ◽  
Analytical Method ◽  
Environmentally Friendly ◽  
Total Reflection ◽  
X Ray ◽  
Subsequent Determination ◽  
Environmentally Friendly Method

A simple, fast and environmentally friendly method for sample preparation and subsequent determination of minerals in wheat flour using TXRF.

MO-FG-BRA-02: Modulation of Clinical Orthovoltage X-Ray Spectrum Further Enhances Radiosensitization of Cancer Cells Targeted with Gold Nanoparticles

2015 ◽  
Vol 42 (6Part29) ◽  
pp. 3564-3565
Author(s):  
T Wolfe ◽  
F Reynoso ◽  
J Cho ◽  
C Quini ◽  
M Cortez ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cancer Cells ◽  
X Ray
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