scholarly journals Endothelial monolayer permeability under controlled oxygen tension

2017 ◽  
Vol 9 (6) ◽  
pp. 529-538 ◽  
Author(s):  
Kenichi Funamoto ◽  
Daisuke Yoshino ◽  
Kento Matsubara ◽  
Ioannis K. Zervantonakis ◽  
Kiyoe Funamoto ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Oxygen Tension ◽  
Cell Monolayer ◽  
Endothelial Permeability ◽  
Cell Junction ◽  
Endothelial Cell Monolayer ◽  
Endothelial Monolayer ◽  
Monolayer Permeability ◽  
Cell Cell

Exposure of endothelial cell monolayer to hypoxia leads to internalization of VE-cadherins, loss of cell–cell junction integrity, and increased endothelial permeability.

scholarly journals Rap1 Is Involved in Angiopoietin-1-Induced Cell-Cell Junction Stabilization and Endothelial Cell Sprouting

Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 155
Author(s):  
Vanda Gaonac’h-Lovejoy ◽  
Cécile Boscher ◽  
Chantal Delisle ◽  
Jean-Philippe Gratton
Keyword(s):  
Endothelial Cell ◽  
Endothelial Permeability ◽  
Intercellular Junctions ◽  
Small Gtpase ◽  
Endothelial Barrier ◽  
Cell Junction ◽  
Barrier Integrity ◽  
Rap1 Activation ◽  
Angiopoietin 1 ◽  
Cell Cell

Angiopoietin-1 (Ang-1) is an important proangiogenic factor also involved in the maintenance of endothelial-barrier integrity. The small GTPase Rap1 is involved in the regulation of adherens junctions through VE-cadherin-mediated adhesion, and in endothelial permeability. While many studies established that Rap1 activation is critical for endothelial cell–cell adhesions, its roles in the antipermeability effects of Ang-1 are ill-defined. Thus, we determined the contribution of Rap1 to Ang-1-stimulated angiogenic effects on endothelial cells (ECs). We found that Rap1 is activated following Ang-1 stimulation and is required for the antipermeability effects of Ang-1 on EC monolayers. Our results also revealed that Rap1 is necessary for EC sprouting stimulated by Ang-1 but had no significant effect on Ang-1-induced EC migration and adhesion. In contrast, downregulation of VE-cadherin markedly increased the adhesiveness of ECs to the substratum, which resulted in inhibition of Ang-1-stimulated migration. These results revealed that Rap1 is central to the effects of Ang-1 at intercellular junctions of ECs, whereas VE-cadherin is also involved in the adhesion of ECs to the extracellular matrix.

2D34 Investigation of Permeability of Endothelial Cell Monolayer by Using Microfluidic Device with Controllability of Oxygen Tension

2016 ◽  
Vol 2016.28 (0) ◽  
pp. _2D34-1_-_2D34-5_
Author(s):  
Kento MATSUBARA ◽  
Kenichi FUNAMOTO ◽  
Ioannis K. ZERVANTONAKIS ◽  
Kiyoe FUNAMOTO ◽  
Takuya ITO ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Oxygen Tension ◽  
Microfluidic Device ◽  
Cell Monolayer ◽  
Endothelial Cell Monolayer

Aluminum effects on brain microvessel endothelial cell monolayer permeability

1988 ◽  
Vol 45 (3) ◽  
pp. 249-257 ◽  
Author(s):  
Kenneth L. Audus ◽  
Judy A. Shinogle ◽  
François L. Guillot ◽  
Sheila R. Holthaus
Keyword(s):  
Endothelial Cell ◽  
Cell Monolayer ◽  
Endothelial Cell Monolayer ◽  
Microvessel Endothelial Cell ◽  
Monolayer Permeability ◽  
Brain Microvessel
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