scholarly journals Real-time quantification of endothelial response to shear stress and vascular modulators

2017 ◽  
Vol 9 (4) ◽  
pp. 362-374 ◽  
Author(s):  
Jackson G. DeStefano ◽  
Ashley Williams ◽  
Alexa Wnorowski ◽  
Nahom Yimam ◽  
Peter C. Searson ◽  
...  
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Real Time ◽  
Cell Morphology ◽  
Applied Shear Stress ◽  
Endothelial Response

Real-time quantification of endothelial cell morphology and activity under applied shear stress.

Effects of Steady Spatial Wall Shear Stress Gradients on Endothelial Cell Morphology in Three-Dimensional Models

2007 ◽  
Author(s):  
Leonie Rouleau ◽  
Monica Farcas ◽  
Jean-Claude Tardif ◽  
Rosaire Mongrain ◽  
Richard Leask
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Endothelial Cell ◽  
Wall Shear Stress ◽  
Cell Morphology ◽  
Flow Direction ◽  
Stress Gradient ◽  
Spatial Gradient ◽  
Stress Gradients ◽  
Wall Shear

Endothelial cell (EC) dysfunction has been linked to atherosclerosis through their response to hemodynamic forces. Flow in stenotic vessels creates complex spatial gradients in wall shear stress. In vitro studies examining the effect of shear stress on endothelial cells have used unrealistic and simplified models, which cannot reproduce physiological conditions. The objective of this study was to expose endothelial cells to the complex shear shear pattern created by an asymmetric stenosis. Endothelial cells were grown and exposed for different times to physiological steady flow in straight dynamic controls and in idealized asymmetric stenosis models. Cells subjected to 1D flow aligned with flow direction and had a spindle-like shape when compared to static controls. Endothelial cell morphology was noticeable different in the regions with a spatial gradient in wall shear stress, being more randomly oriented and of cobblestone shape. This occurred despite the presence of an increased magnitude in shear stress. No other study to date has described this morphology in the presence of a positive wall shear stress gradient or gradient of significant shear magnitude. This technique provides a more realistic model to study endothelial cell response to spatial and temporal shear stress gradients that are present in vivo and is an important advancement towards a better understanding of the mechanisms involved in coronary artery disease.

Endothelial cell morphology and response to shear stress in an asymmetric stenosis model

2006 ◽  
Vol 39 ◽  
pp. S312 ◽  
Author(s):  
L. Rouleau ◽  
M. Farcas ◽  
J.-C. Tardif ◽  
E. Thorin ◽  
R. Mongrain ◽  
...  
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Cell Morphology

Shear Stress Induces Changes in Cerebral Endothelial Cell Morphology and Cytoskeletal Anatomy

Neurosurgery ◽  
1992 ◽  
Vol 30 (5) ◽  
pp. 805 ◽  
Author(s):  
A. Reisner ◽  
P. R. Girard ◽  
D. M. Polk ◽  
J. J. Olson
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Cell Morphology ◽  
Cerebral Endothelial Cell
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