Urinary metabolomic study of the antagonistic effect of P. ginseng in rats with estrogen decline using ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

2018 ◽  
Vol 9 (3) ◽  
pp. 1444-1453 ◽  
Author(s):  
He Lin ◽  
Zhongying Liu ◽  
Zifeng Pi ◽  
Lihui Men ◽  
Weijia Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Panax Ginseng ◽  
Metabolic Pathways ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Antagonistic Effect ◽  
Flight Mass Spectrometry ◽  
Metabolomic Study

This study investigates the antagonistic effect of Panax ginseng in rats with estrogen decline by its intervention in some major endogenous metabolic pathways.

scholarly journals Metabolism studies of paeoniflorin in rat liver microsomes by ultra-performance liquid chromatography coupled with hybrid quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS)

2018 ◽  
Vol 5 (10) ◽  
pp. 180759
Author(s):  
L. J. Zhu ◽  
S. S. Sun ◽  
Y. X. Hu ◽  
Y. F. Liu
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Rat Liver ◽  
Metabolic Pathways ◽  
Liver Microsomes ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Rat Liver Microsomes ◽  
Flight Mass Spectrometry ◽  
Tof Ms

To explore metabolism mechanism of paeoniflorin in the liver and further understand intact metabolism process of paeoniflorin, a rapid, convenient and effective assay is described using ultra-performance liquid chromatography coupled with hybrid quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS). The strategy was confirmed in the following primary processes: firstly, different concentration of paeoniflorin, rat liver microsomes, coenzymes and different incubated conditions were optimized to build a biotransformation model of rat liver microsomes in vitro by high performance liquid chromatography with diode array detection (HPLC-DAD); secondly, the metabolites of paeoniflorin in rat liver microsomes were detected and screened using UPLC-Q-TOF-MS/MS by comparing the total ion chromatogram (TIC) of the experimental group with those of control groups; finally, the molecular formulae and corresponding chemical structures of paeoniflorin metabolites were identified by comparing the MS and MS/MS spectra with the self-constructed database and simulation software. Based on this analytical strategy, 20 metabolites of paeoniflorin were found and 6 metabolites (including four new compounds) were tentatively identified. It was shown that hydrolysis and oxidation were the major metabolic pathways of paeoniflorin in rat liver microsomes, and the main metabolic sites were the structures of pinane and the ester bond. These findings were significant for a better understanding of the metabolism of paeoniflorin in rat liver microsomes and the proposed metabolic pathways of paeoniflorin might provide fundamental support for the further research in the pharmacological mechanism of Paeoniae Radix Rubra (PRR).

Metabolomic study of corticosterone-induced cytotoxicity in PC12 cells by ultra performance liquid chromatography-quadrupole/time-of-flight mass spectrometry

2016 ◽  
Vol 12 (3) ◽  
pp. 902-913 ◽  
Author(s):  
Hongye Zhang ◽  
Hua Zheng ◽  
Gan Zhao ◽  
Chaoling Tang ◽  
Shiyin Lu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Pc12 Cells ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Flight Mass Spectrometry ◽  
Metabolomic Study ◽  
Cell Metabolomics ◽  
Tof Ms

UPLC-Q/TOF-MS-based cell metabolomics illustrates dysfunction of multiple pathways was involved in the corticosterone-induced cytotoxicity in PC12 cells.

Sign in / Sign up

Export Citation Format

Share Document