scholarly journals The composition of potentially bioactive triterpenoid glycosides in red raspberry is influenced by tissue, extraction procedure and genotype

2017 ◽  
Vol 8 (10) ◽  
pp. 3469-3479 ◽  
Author(s):  
Gordon J. McDougall ◽  
J. William Allwood ◽  
Gema Pereira-Caro ◽  
Emma M. Brown ◽  
Cheryl Latimer ◽  
...  
Keyword(s):  
Extraction Procedure ◽  
Red Raspberry ◽  
Tissue Extraction ◽  
Triterpenoid Glycosides ◽  
Different Tissues

The composition of potentially bioactive triterpenoids differs between different tissues and varieties of raspberries, but are readily available from juices and purees.

scholarly journals Extraction Methods, Characterization and Biomedical Applications of Collagen: a Review

2021 ◽  
Vol 11 (5) ◽  
pp. 13587-13613
Keyword(s):  
Tissue Engineering ◽  
Extraction Method ◽  
Biomedical Applications ◽  
Extraction Procedure ◽  
Extraction Methods ◽  
Collagen Types ◽  
Advantages And Disadvantages ◽  
Native Collagen ◽  
Isolation Methods ◽  
Different Tissues

It is difficult to develop a standard extraction method for all types of collagen from different tissues due to the extreme diversity of collagen types. Some procedures are based on the isolation of acid, pepsin, and enzymatic soluble collagen, showing certain advantages and disadvantages. Other methods were also optimized to partially purify collagen and extract it easier than the methods currently used. Indeed, this review describes some advantages and disadvantages of these isolation methods. Moreover, major biomedical applications of collagen were reported. Given the great importance of biocompatible matrices in tissue engineering, the availability of native collagen should be investigated by refining the collagen extraction procedure.

A Physiologic Regulator of Collagen-Induced Platelet Aggregation: Inhibition by Clq

1981 ◽  
Vol 45 (02) ◽  
pp. 110-115 ◽  
Author(s):  
György Csákó ◽  
Eva A Suba
Keyword(s):  
Platelet Aggregation ◽  
Human Platelet ◽  
Platelet Reactivity ◽  
High Specificity ◽  
Turbidimetric Method ◽  
Specific Manner ◽  
Aggregation Inhibition ◽  
Different Tissues

SummaryPlatelet aggregations were studied by a turbidimetric method in citrated human platelet-rich plasmas (PRP) in vitro. Human Clq inhibited the aggregations caused by collagens derived from different tissues and species. Clq was needed by weight in comparable quantities to collagen for neutralizing the aggregating effect. The dependence of the inhibitory reaction on the preincubation of platelets with Clq and the differences in the occurrence of aggregating substances in supernatants of PRP triggered with collagen in the presence or absence of Clq, confirmed that Clq exerts its effect by preventing fixation of collagen to platelets. In addition, the high specificity of the inhibitory action of Clq for collagen-induced platelet aggregation was demonstrated by results obtained for testing a variety of aggregating agents in combination with Clq and/or collagen.Since normal concentrations of Clq in the blood are in the range of inhibitory doses of Clq for collagen-induced platelet aggregations in vitro and upon activation of complement Clq is known to dissociate from Cl, it is proposed that Clq may participate in a highly specific manner in regulating platelet reactivity to collagen in vivo.

Evaluation and application of BIOPOLE, a biopsy device for in situ non-lethal tissue extraction for fishes

2018 ◽  
Vol 595 ◽  
pp. 149-156 ◽  
Author(s):  
JG Smith ◽  
D Malone ◽  
DS Baetscher ◽  
MH Carr
Keyword(s):  
Tissue Extraction ◽  
Biopsy Device

DIRECT MEASUREMENT OF ARGININE-VASOPRESSIN IN HUMAN SERUM WITHOUT EXTRACTION PROCEDURE

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S130 ◽  
Author(s):  
H. Wagner ◽  
V. Maier ◽  
H.-J. Herrmann ◽  
H. E. Franz
Keyword(s):  
Human Serum ◽  
Direct Measurement ◽  
Arginine Vasopressin ◽  
Extraction Procedure

HPLC analysis and activity to determine the effect of drying and extraction on Ficus benghalensis leaves

2020 ◽  
Vol 9 (1) ◽  
pp. 6-10
Author(s):  
Nithyakalyani K
Keyword(s):  
Extraction Method ◽  
Chemical Constituents ◽  
Extraction Procedure ◽  
Chemical Constituent ◽  
Chemical Components ◽  
Drying Method ◽  
Ultrasound Assisted Extraction ◽  
Critical Stage ◽  
Ficus Benghalensis ◽  
Assisted Extraction

Ficus benghalensis is one of those taboo plants in India, which was claimed to be possessed and have weird effects on human health. Apart from this ficus species has a great variety of chemical constituents and an abundant amount of antioxidants. Drying is the most critical stage of improving the activity or preventing the loss of chemical components from a drug. There is another stage of ensuring high chemical constituent content in the plant and that is the extraction procedure. So the point of focus in the current research is to find the effect of extraction method and drying on the anti-inflammatory potential of the plant. The result of the extraction method and drying method of the plant was investigated and found that the ultrasound-assisted extraction of the shade dried leaves was found to give the highest yield of flavonoids and activity.

Differential Expression and Assembly Mode of Glutamine Synthetase Isoen-zymes in Different Tissues and Organs of Maize

2017 ◽  
Vol 43 (9) ◽  
pp. 1410 ◽  
Author(s):  
Xiao-Chun WANG ◽  
Hao-Ran ZHANG ◽  
Yi-Hao WEI ◽  
Xi-Ting JIA ◽  
Ming-Xin GU ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Differential Expression ◽  
Different Tissues

744 PB 076 IDENTIFICATION OF BOTH ACID AND NEUTRAL INVERTASE ACTIVITY IN DEVELOPING STRAWBERRY FRUIT

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 539f-539
Author(s):  
Kirk W. Pomper ◽  
Patrick J. Breen
Keyword(s):  
Extraction Procedure ◽  
Invertase Activity ◽  
Assimilate Transport ◽  
Strawberry Fruit ◽  
Fruit Extracts ◽  
Neutral Invertase ◽  
Optimum Ph ◽  
Cold Acetone ◽  
Ripe Stage ◽  
Sugar Levels

Invertase (INV) may influence sugar levels and assimilate transport in strawberry fruit. Several groups, including our own, have only detected acid INV (optimum pH 4.6) in strawberry fruit, however, recently Hubbard et al. (Physiol. Plant. 82:191-196, 1991) reported the presence of a neutral INV (pH 7.5). Since dissimilar isolation protocols may have contributed to the different findings, we re-examined our work with developing `Brighton' strawberry using the extraction procedure of Hubbard et al. Neutral INV activity per gFW (pH 7.5-8.0) increased many fold as fruit developed from green to the red ripe stage. Acid INV activity decreased markedly from green-white to the red stage. In addition, when fruit extracts were precipitated with cold acetone, a pellet contained 60% of the acid INV activity, and a surface coagulation of protein contained 60% of the neutral INV activity. This allowed easy separation of these two enzymes. Extraction methodologies affect isolation of neutral INV activity from strawberry fruit.

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