Direct surface-enhanced Raman scattering (SERS) spectroscopy of nucleic acids: from fundamental studies to real-life applications

2018 ◽  
Vol 47 (13) ◽  
pp. 4909-4923 ◽  
Author(s):  
Eduardo Garcia-Rico ◽  
Ramon A. Alvarez-Puebla ◽  
Luca Guerrini
Keyword(s):  
Raman Scattering ◽  
Nucleic Acids ◽  
Real Life ◽  
Surface Enhanced Raman Scattering ◽  
Cutting Edge ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

In this tutorial review, we summarize and discuss the most recent cutting-edge research in the field of direct surface-enhanced Raman scattering (SERS) analysis of nucleic acids.

scholarly journals 60-nt DNA Direct Detection without Pretreatment by Surface-Enhanced Raman Scattering with Polycationic Modified Ag Microcrystal Derived from AgCl Cube

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6790
Author(s):  
Jikai Mao ◽  
Lvtao Huang ◽  
Li Fan ◽  
Fang Chen ◽  
Jingan Lou ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Direct Detection ◽  
Pure Water ◽  
Real Life ◽  
Surface Enhanced Raman Scattering ◽  
Hereditary Diseases ◽  
Label Free Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Direct detection of long-strand DNA by surface-enhanced Raman scattering (SERS) is a valuable method for diagnosis of hereditary diseases, but it is currently limited to less than 25-nt DNA strand in pure water, which makes this approach unsuitable for many real-life applications. Here, we report a 60-nt DNA label-free detection strategy without pretreatment by SERS with polyquaternium-modified Ag microcrystals derived from an AgCl cube. Through the reduction-induced decomposition, the size of the about 3 × 3 × 3 μm3 AgCl cube is reduced to Ag, and the surface is distributed with the uniform size of 63 nm silver nanoparticles, providing a large area of a robust and highly electromagnetic enhancement region. The modified polycationic molecule enhances the non-specific electrostatic interaction with the phosphate group, thereby anchoring DNA strands firmly to the SERS enhanced region intactly. As a result, the single-base recognition ability of this strategy reaches 60-nt and is successfully applied to detect thalassemia-related mutation genes.

Nanostructure-based surface-enhanced Raman scattering biosensors for nucleic acids and proteins

2016 ◽  
Vol 4 (10) ◽  
pp. 1757-1769 ◽  
Author(s):  
Jie Chao ◽  
Wenfang Cao ◽  
Shao Su ◽  
Lixing Weng ◽  
Shiping Song ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Raman Scattering ◽  
Nucleic Acids ◽  
Protein Detection ◽  
Surface Enhanced Raman Scattering ◽  
Label Free ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Nanostructure-based SERS platforms have been developed for nucleic acid and protein detection ranging from label-free, labeled and multiplex analyses.

scholarly journals Ultrasensitive detection of genomic DNA from apple leaf tissues, using surface-enhanced Raman scattering

2011 ◽  
Vol 25 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Cristina M. Muntean ◽  
Nicolae Leopold ◽  
Adela Halmagyi ◽  
Sergiu Valimareanu
Keyword(s):  
Raman Scattering ◽  
Nucleic Acids ◽  
Structural Changes ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced Raman Spectroscopy ◽  
Ultrasensitive Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Genomic Dnas

Ultrasensitive detection of nucleic acids is demonstrated through exploiting the effect of surface-enhanced Raman scattering (SERS). In this work the SERS spectra of eight genomic DNAs from leaves of apple trees grown in the field (Malus domesticaBorkh., Fam.Rosaceae, cultivars. Florina, Idared, Rebra, Goldrush, Romus 3, Romus 4 and the rootstocks M9 and M26) have been analyzed in the wavenumber range 200–1800 cm–1. SERS signatures, spectroscopic band assignments and structural interpretations of these plant genomic DNAs are reported. SERS spectra of nucleic acids are compared here with caution, because these signals are time-dependent and are strongly influenced by DNA amount in the measured sample volume. Similarities of the SERS spectra of genomic DNAs extracted from apple leaves of Goldrush, Rebra and Florina cultivars, respectively, have been detected. Besides, the SERS spectra, corresponding to DNA from M9, Romus 4 and M26, leaves, respectively, show similar features and well resolved bands. Based on this work, specific plant DNA-ligand interactions or DNA structural changes induced by plant stress conditions associated with their natural environment, might be further investigated using surface-enhanced Raman spectroscopy.

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