A new scaling for the rotational diffusion of molecular probes in polymer solutions

2017 ◽  
Vol 19 (48) ◽  
pp. 32687-32697 ◽  
Author(s):  
Jing Qing ◽  
Anpu Chen ◽  
Nanrong Zhao
Keyword(s):  
Diffusion Coefficient ◽  
Theoretical Study ◽  
Polymer Solutions ◽  
Rotational Diffusion ◽  
Molecular Probes ◽  
Dilute Polymer Solutions ◽  
Rotational Diffusion Coefficient

In the present work, we propose a new scaling form for the rotational diffusion coefficient of molecular probes in semi-dilute polymer solutions, based on a theoretical study.

Study on Nanoparticle Sizing Using Fluorescent Polarization Method with DNA Fluorescent Probe

2015 ◽  
Vol 9 (5) ◽  
pp. 534-540 ◽  
Author(s):  
Terutake Hayashi ◽  
◽  
Yuki Ishizaki ◽  
Masaki Michihata ◽  
Yasuhiro Takaya ◽  
...  
Keyword(s):  
Diffusion Coefficient ◽  
Fluorescent Probe ◽  
Diffusion Coefficients ◽  
Hard Spheres ◽  
Rotational Diffusion ◽  
Polarization Method ◽  
Novel Method ◽  
Rotational Diffusion Coefficients ◽  
20 Nm ◽  
Rotational Diffusion Coefficient

Fluorescent polarization methods are used to detect complementary base pairing of DNA in biological fields. These methods work by measuring the rotational diffusion coefficient of Brownian motion of the fluorescent particles in solution. The rotational diffusion coefficient corresponds to the inverse third power of diameter according to the Debye-Stokes-Einstein equation for nanoparticles as hard spheres. We develop a novel method to measure the rotational diffusion coefficient using a fluorescent probe with a DNA spacer connected to a gold nanoparticle. We studied the physical characteristics of this probe to verify the feasibility of the proposed method. The rotational diffusion coefficients of gold nanoparticles with diameters ranging between 5–20 nm were measured using this developed system. In this manuscript we describe a novel fluorescent polarization method for nanoparticle sizing using a fluorescent DNA probe.

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