Microsecond resolved single-molecule FRET time series measurements based on the line confocal optical system combined with hybrid photodetectors

Hiroyuki Oikawa; Takumi Takahashi; Supawich Kamonprasertsuk; Satoshi Takahashi

doi:10.1039/c7cp06268k

Microsecond resolved single-molecule FRET time series measurements based on the line confocal optical system combined with hybrid photodetectors

Physical Chemistry Chemical Physics ◽

10.1039/c7cp06268k ◽

2018 ◽

Vol 20 (5) ◽

pp. 3277-3285 ◽

Cited By ~ 2

Author(s):

Hiroyuki Oikawa ◽

Takumi Takahashi ◽

Supawich Kamonprasertsuk ◽

Satoshi Takahashi

Keyword(s):

Time Series ◽

Confocal Microscopy ◽

Single Molecule ◽

Optical System ◽

Time Resolution ◽

Observation Time ◽

Single Molecule Fret

Line confocal microscopy combined with hybrid photodetectors achieves a time resolution of 10 μs and an observation time of approximately 5 ms in single-molecule FRET time series measurements.

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Camera-based single-molecule FRET detection with improved time resolution

Physical Chemistry Chemical Physics ◽

10.1039/c5cp04137f ◽

2015 ◽

Vol 17 (41) ◽

pp. 27862-27872 ◽

Cited By ~ 28

Author(s):

Shazia Farooq ◽

Johannes Hohlbein

Keyword(s):

Probability Distribution ◽

Single Molecule ◽

Time Resolution ◽

Laser Excitation ◽

Single Molecule Detection ◽

Distribution Analysis ◽

Single Molecule Fret ◽

Alternating Laser Excitation

Here the authors report on significant improvements in time-resolution and throughput in camera-based single-molecule detection by combining stroboscopic alternating-laser excitation with dynamic probability distribution analysis.

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1J1412 Variational Bayes-Hidden Markov Model for time-stamped single-molecule FRET time series data to analyze conformational state dynamics(Measurements,The 49th Annual Meeting of the Biophysical Society of Japan)

Seibutsu Butsuri ◽

10.2142/biophys.51.s55_3 ◽

2011 ◽

Vol 51 (supplement) ◽

pp. S55

Author(s):

Kenji Okamoto ◽

Yasushi Sako

Keyword(s):

Time Series ◽

Markov Model ◽

Annual Meeting ◽

Single Molecule ◽

Time Series Data ◽

Hidden Markov ◽

Conformational State ◽

Series Data ◽

Single Molecule Fret ◽

Biophysical Society

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Faculty Opinions recommendation of Learning rates and states from biophysical time series: a Bayesian approach to model selection and single-molecule FRET data.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.2278956.1900054 ◽

2010 ◽

Author(s):

Stuart S Licht

Keyword(s):

Time Series ◽

Model Selection ◽

Single Molecule ◽

Bayesian Approach ◽

Single Molecule Fret ◽

Learning Rates

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Learning Rates and States from Biophysical Time Series: A Bayesian Approach to Model Selection and Single-Molecule FRET Data

Biophysical Journal ◽

10.1016/j.bpj.2009.09.031 ◽

2009 ◽

Vol 97 (12) ◽

pp. 3196-3205 ◽

Cited By ~ 235

Author(s):

Jonathan E. Bronson ◽

Jingyi Fei ◽

Jake M. Hofman ◽

Ruben L. Gonzalez ◽

Chris H. Wiggins

Keyword(s):

Time Series ◽

Model Selection ◽

Single Molecule ◽

Bayesian Approach ◽

Single Molecule Fret ◽

Learning Rates

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Quantitative Evaluation of Cross Correlation Between Two Finite-Length Time Series with Applications to Single-Molecule FRET

The Journal of Physical Chemistry B ◽

10.1021/jp804440y ◽

2008 ◽

Vol 112 (44) ◽

pp. 13962-13970 ◽

Cited By ~ 12

Author(s):

Jeffery A. Hanson ◽

Haw Yang

Keyword(s):

Time Series ◽

Quantitative Evaluation ◽

Finite Length ◽

Single Molecule ◽

Cross Correlation ◽

Single Molecule Fret

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Faculty Opinions recommendation of Single-molecule FRET reveals the folding dynamics of the human telomerase RNA pseudoknot domain.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.724844428.793511237 ◽

2015 ◽

Author(s):

Lea Harrington

Keyword(s):

Single Molecule ◽

Telomerase Rna ◽

Single Molecule Fret ◽

Folding Dynamics ◽

Human Telomerase ◽

Rna Pseudoknot

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Faculty Opinions recommendation of Single-molecule FRET unveils induced-fit mechanism for substrate selectivity in flap endonuclease 1.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.727337515.793539178 ◽

2017 ◽

Author(s):

Antoine van Oijen

Keyword(s):

Single Molecule ◽

Substrate Selectivity ◽

Induced Fit ◽

Flap Endonuclease 1 ◽

Single Molecule Fret

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Encoding Multiple Virtual Signals in DNA Barcodes with Single-Molecule FRET

Nano Letters ◽

10.1021/acs.nanolett.0c04502 ◽

2021 ◽

Vol 21 (4) ◽

pp. 1694-1701 ◽

Cited By ~ 1

Author(s):

Sung Hyun Kim ◽

Hyunwoo Kim ◽

Hawoong Jeong ◽

Tae-Young Yoon

Keyword(s):

Single Molecule ◽

Dna Barcodes ◽

Single Molecule Fret

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Single-Molecule FRET-Based Dynamic DNA Sensor

ACS Sensors ◽

10.1021/acssensors.1c00002 ◽

2021 ◽

Author(s):

Anoja Megalathan ◽

Kalani M. Wijesinghe ◽

Soma Dhakal

Keyword(s):

Single Molecule ◽

Dna Sensor ◽

Single Molecule Fret

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Direct observation of effect of crowding induced macromolecular hydration on molecular breathing in the stem of Fork-DNA by single-molecule FRET microspectroscopy

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2020.11.197 ◽

2021 ◽

Vol 167 ◽

pp. 559-569

Author(s):

Soma Mondal ◽

Padmaja P. Mishra

Keyword(s):

Direct Observation ◽

Single Molecule ◽

Single Molecule Fret

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