Probe-location dependent resonance energy transfer at lipid/water interfaces: comparison between the gel- and fluid-phase of lipid bilayer

2017 ◽  
Vol 19 (38) ◽  
pp. 25870-25885 ◽  
Author(s):  
Moirangthem Kiran Singh ◽  
Mohammad Firoz Khan ◽  
Him Shweta ◽  
Sobhan Sen
Keyword(s):  
Energy Transfer ◽  
Lipid Bilayer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluid Phase ◽  
Lipid Fluidity ◽  
Probe Location ◽  
Donor Acceptor ◽  
Dielectric Environment

Effect of dielectric environment and lipid fluidity/rigidity in multi-chromophoric FRET from a series of donors to acceptors at lipid/water interfaces are monitored by tailored donor–acceptor pairs.

Quantum Dot Bioconjugates as Energy Donors in Fluorescence Resonance Energy Transfer Assays

2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Genetically Engineered ◽  
Molar Ratio ◽  
Binding Assays ◽  
Specific Sequence ◽  
Donor Acceptor

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.

Controlling fluorescence resonance energy transfer of donor–acceptor dyes by Diels–Alder dynamic covalent bonds

2021 ◽  
Vol 57 (26) ◽  
pp. 3275-3278
Author(s):  
Yanhui Cui ◽  
Fen Li ◽  
Xin Zhang
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Aromatic Amine ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Diels Alder ◽  
Covalent Bonds ◽  
Donor Acceptor ◽  
Fluorescence Resonance ◽  
Mutual Conversion

Two new dyes, consisting of an aromatic amine donor and dansyl acceptor connected by Diels–Alder bonds, display a switchable energy transfer. Dynamic covalent properties enable the mutual conversion of the two dyes by maleimide exchanges.

scholarly journals Ureasil organic–inorganic hybrids as photoactive waveguides for conjugated polyelectrolyte luminescent solar concentrators

2017 ◽  
Vol 1 (11) ◽  
pp. 2271-2282 ◽  
Author(s):  
Ilaria Meazzini ◽  
Camille Blayo ◽  
Jochen Arlt ◽  
Ana-Teresa Marques ◽  
Ullrich Scherf ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Solar Concentrators ◽  
Conjugated Polyelectrolyte ◽  
Luminescent Solar Concentrators ◽  
Donor Acceptor

We test the potential of resonance energy transfer to enhance the performance of conjugated copolyelectrolyte donor–acceptor luminescent solar concentrators immobilised within a photoactive organic–inorganic ureasil waveguide.

Synthesis of Lipid-Quantum Dots and the Fluorescence Resonance Energy Transfer of Quantum Dots in Lipid Bilayer

2011 ◽  
Vol 399-401 ◽  
pp. 646-649
Author(s):  
Chao Ye ◽  
Chen Gui Li ◽  
Yun Qing Wang ◽  
Jie Yu ◽  
Fang Zhou ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Lipid Bilayer ◽  
Fluorescence Resonance Energy Transfer ◽  
Phase Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Excitation Wavelength ◽  
Quenching Efficiency ◽  
Fluorescence Resonance

Hydrophilic CdTe and NIR CdHgTe QDs were successfully transferred from aqueous solution to nonpolar chloroform solvent via efficient phase transfer reagent to synthesize lipid-QDs. The liposomes showed bright fluorescence under the fluorescence microscope. It was confirmed that fluorescence resonance energy transfer (FRET) occurred between two types of QDs encapsulated in lipid bilayer with an excitation wavelength of 370nm, and the FRET efficiencies were satisfied (quenching efficiency was 51% and enhance efficiency was 40%), which laid a foundation for the research of drug release from liposomes.

scholarly journals Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer

2004 ◽  
Vol 381 (1) ◽  
pp. 307-312 ◽  
Author(s):  
Satoshi KARASAWA ◽  
Toshio ARAKI ◽  
Takeharu NAGAI ◽  
Hideaki MIZUNO ◽  
Atsushi MIYAWAKI
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Fluorescent Proteins ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Acceptor Pair ◽  
Donor And Acceptor ◽  
Donor Acceptor ◽  
Donor Acceptor Pair ◽  
Fluorescence Resonance

GFP (green fluorescent protein)-based FRET (fluorescence resonance energy transfer) technology has facilitated the exploration of the spatio-temporal patterns of cellular signalling. While most studies have used cyan- and yellow-emitting FPs (fluorescent proteins) as FRET donors and acceptors respectively, this pair of proteins suffers from problems of pH-sensitivity and bleeding between channels. In the present paper, we demonstrate the use of an alternative additional donor/acceptor pair. We have cloned two genes encoding FPs from stony corals. We isolated a cyan-emitting FP from Acropara sp., whose tentacles exhibit cyan coloration. Similar to GFP from Renilla reniformis, the cyan FP forms a tight dimeric complex. We also discovered an orange-emitting FP from Fungia concinna. As the orange FP exists in a complex oligomeric structure, we converted this protein into a monomeric form through the introduction of three amino acid substitutions, recently reported to be effective for converting DsRed into a monomer (Clontech). We used the cyan FP and monomeric orange FP as a donor/acceptor pair to monitor the activity of caspase 3 during apoptosis. Due to the close spectral overlap of the donor emission and acceptor absorption (a large Förster distance), substantial pH-resistance of the donor fluorescence quantum yield and the acceptor absorbance, as well as good separation of the donor and acceptor signals, the new pair can be used for more effective quantitative FRET imaging.

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