Kinetics and heterogeneity of energy transfer from light harvesting complex II to photosystem I in the supercomplex isolated from Arabidopsis

2017 ◽  
Vol 19 (13) ◽  
pp. 9210-9222 ◽  
Author(s):  
Stefano Santabarbara ◽  
Tania Tibiletti ◽  
William Remelli ◽  
Stefano Caffarri
Keyword(s):  
Energy Transfer ◽  
Photosystem I ◽  
Light Harvesting ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

Energy transfer from the LHCII when associated with the PSI–LHCI is heterogeneous and characterised by macroscopic transfer of ∼55 ns−1 and 15 ns−1, respectively.

scholarly journals Observation of dissipative chlorophyll-to-carotenoid energy transfer in light-harvesting complex II in membrane nanodiscs

2019 ◽  
Author(s):  
Minjung Son ◽  
Alberta Pinnola ◽  
Samuel C. Gordon ◽  
Roberto Bassi ◽  
Gabriela S. Schlau-Cohen
Keyword(s):  
Energy Transfer ◽  
Conformational Changes ◽  
Light Harvesting ◽  
Electronic Spectroscopy ◽  
Local Environment ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii ◽  
Photosynthetic Antenna

<pre><p><a></a>Green plants prevent photodamage under high light conditions by dissipating excess energy as heat. Conformational changes of the photosynthetic antenna complexes activate dissipation by leveraging the sensitivity of the photophysics of the chlorophyll and carotenoids to their surrounding protein. However, the mechanisms and site of dissipation are still debated, largely due to two challenges. First, because of the ultrafast timescales and large energy gaps involved, measurements lacked the temporal or spectral requirements. Second, experiments have been performed in detergent, which can induce non-native conformations, or <i>in vivo</i>, where contributions from the multiple complexes cannot be disentangled and are further obfuscated by laser-induced artifacts. Here, we overcome both challenges by applying ultrabroadband two-dimensional electronic spectroscopy to the principal antenna complex, light-harvesting complex II, in a near-native membrane. The membrane enhances two dissipative pathways, one of which was previously uncharacterized chlorophyll-to-carotenoid energy transfer. Our results highlight the sensitivity of the photophysics to the local environment, which may be used to control the balance between light harvesting and dissipation <i>in vivo</i>.</p></pre>

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