Synthetic cells produce a quorum sensing chemical signal perceived byPseudomonas aeruginosa

2018 ◽  
Vol 54 (17) ◽  
pp. 2090-2093 ◽  
Author(s):  
Giordano Rampioni ◽  
Francesca D’Angelo ◽  
Marco Messina ◽  
Alessandro Zennaro ◽  
Yutetsu Kuruma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Quorum Sensing ◽  
Chemical Signal ◽  
Giant Vesicles ◽  
Enzymatic Production ◽  
Bacterial Quorum Sensing ◽  
Synthetic Cells ◽  
Bacterial Quorum

A minimal gene expression machinery is encapsulated inside giant vesicles, leading to the enzymatic production of a bacterial quorum sensing signal.

scholarly journals Use of Bacterial Quorum-Sensing Components to Regulate Gene Expression in Plants

2006 ◽  
Vol 140 (4) ◽  
pp. 1205-1212 ◽  
Author(s):  
Young-Sook You ◽  
Heather Marella ◽  
Rodolfo Zentella ◽  
Yiyong Zhou ◽  
Tim Ulmasov ◽  
...  
Keyword(s):  
Gene Expression ◽  
Quorum Sensing ◽  
Regulate Gene Expression ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Regulate Gene

scholarly journals Implications of Rewiring Bacterial Quorum Sensing

2007 ◽  
Vol 74 (2) ◽  
pp. 437-445 ◽  
Author(s):  
Eric L. Haseltine ◽  
Frances H. Arnold
Keyword(s):  
Gene Expression ◽  
Quorum Sensing ◽  
Network Architecture ◽  
Vibrio Fischeri ◽  
Cell Communication ◽  
Biologically Relevant ◽  
Synthetic Gene Circuits ◽  
Bacterial Quorum Sensing ◽  
Forward Engineering ◽  
Bacterial Quorum

ABSTRACT Bacteria employ quorum sensing, a form of cell-cell communication, to sense changes in population density and regulate gene expression accordingly. This work investigated the rewiring of one quorum-sensing module, the lux circuit from the marine bacterium Vibrio fischeri. Steady-state experiments demonstrate that rewiring the network architecture of this module can yield graded, threshold, and bistable gene expression as predicted by a mathematical model. The experiments also show that the native lux operon is most consistent with a threshold, as opposed to a bistable, response. Each of the rewired networks yielded functional population sensors at biologically relevant conditions, suggesting that this operon is particularly robust. These findings (i) permit prediction of the behaviors of quorum-sensing operons in bacterial pathogens and (ii) facilitate forward engineering of synthetic gene circuits.

scholarly journals The Vitamin Riboflavin and Its Derivative Lumichrome Activate the LasR Bacterial Quorum-Sensing Receptor

2008 ◽  
Vol 21 (9) ◽  
pp. 1184-1192 ◽  
Author(s):  
Sathish Rajamani ◽  
Wolfgang D. Bauer ◽  
Jayne B. Robinson ◽  
John M. Farrow ◽  
Everett C. Pesci ◽  
...  
Keyword(s):  
Gene Expression ◽  
Quorum Sensing ◽  
Binding Pocket ◽  
Homoserine Lactone ◽  
Docking Studies ◽  
Intercellular Signaling ◽  
Signaling Mechanism ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Regulated Gene Expression

Many bacteria use quorum sensing (QS) as an intercellular signaling mechanism to regulate gene expression in local populations. Plant and algal hosts, in turn, secrete compounds that mimic bacterial QS signals, allowing these hosts to manipulate QS-regulated gene expression in bacteria. Lumichrome, a derivative of the vitamin riboflavin, was purified and chemically identified from culture filtrates of the alga Chlamydomonas as a QS signal-mimic compound capable of stimulating the Pseudomonas aeruginosa LasR QS receptor. LasR normally recognizes the N-acyl homoserine lactone (AHL) signal, N-3-oxo-dodecanoyl homoserine lactone. Authentic lumichrome and riboflavin stimulated the LasR receptor in bioassays and lumichrome activated LasR in gel shift experiments. Amino acid substitutions in LasR residues required for AHL binding altered responses to both AHLs and lumichrome or riboflavin. These results and docking studies indicate that the AHL binding pocket of LasR recognizes both AHLs and the structurally dissimilar lumichrome or riboflavin. Bacteria, plants, and algae commonly secrete riboflavin or lumichrome, raising the possibility that these compounds could serve as either QS signals or as interkingdom signal mimics capable of manipulating QS in bacteria with a LasR-like receptor.

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