scholarly journals Two-color vibrational imaging of glucose metabolism using stimulated Raman scattering

2018 ◽  
Vol 54 (2) ◽  
pp. 152-155 ◽  
Author(s):  
Rong Long ◽  
Luyuan Zhang ◽  
Lingyan Shi ◽  
Yihui Shen ◽  
Fanghao Hu ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Raman Scattering ◽  
Glucose Uptake ◽  
Imaging Technique ◽  
Stimulated Raman Scattering ◽  
Living Cells ◽  
Stimulated Raman

A two-color vibrational imaging technique for simultaneously mapping glucose uptake and incorporation activity inside living cells is reported.

scholarly journals Vibrational Imaging of Glucose Uptake in Live Cells and Tissues by Stimulated Raman Scattering Microscopy

2015 ◽  
Vol 108 (2) ◽  
pp. 480a
Author(s):  
Fanghao Hu ◽  
Zhixing Chen ◽  
Luyuan Zhang ◽  
Yihui Shen ◽  
Lu Wei ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Glucose Uptake ◽  
Stimulated Raman Scattering ◽  
Live Cells ◽  
Stimulated Raman

scholarly journals Vibrational Imaging of Glucose Uptake Activity in Live Cells and Tissues by Stimulated Raman Scattering

2015 ◽  
Vol 54 (34) ◽  
pp. 9821-9825 ◽  
Author(s):  
Fanghao Hu ◽  
Zhixing Chen ◽  
Luyuan Zhang ◽  
Yihui Shen ◽  
Lu Wei ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Glucose Uptake ◽  
Stimulated Raman Scattering ◽  
Live Cells ◽  
Uptake Activity ◽  
Stimulated Raman

scholarly journals Stimulated Raman scattering microscopy for rapid brain tumor histology

2017 ◽  
Vol 10 (05) ◽  
pp. 1730010 ◽  
Author(s):  
Yifan Yang ◽  
Lingchao Chen ◽  
Minbiao Ji
Keyword(s):  
Brain Tumor ◽  
Brain Tumors ◽  
Raman Scattering ◽  
Tumor Imaging ◽  
Imaging Technique ◽  
Stimulated Raman Scattering ◽  
Label Free ◽  
Basic Principles ◽  
Normal Tissues ◽  
Stimulated Raman

Rapid histology of brain tissues with sufficient diagnostic information has the great potential to aid neurosurgeons during operations. Stimulated Raman Scattering (SRS) microscopy is an emerging label-free imaging technique, with the intrinsic chemical resolutions to delineate brain tumors from normal tissues without the need of time-consuming tissue processing. Growing number of studies have shown SRS as a “virtual histology” tool for rapid diagnosis of various types of brain tumors. In this review, we focus on the basic principles and current developments of SRS microscopy, as well as its applications for brain tumor imaging.

scholarly journals Intraoperative assessment of skull base tumors using stimulated Raman scattering microscopy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kseniya S. Shin ◽  
Andrew T. Francis ◽  
Andrew H. Hill ◽  
Mint Laohajaratsang ◽  
Patrick J. Cimino ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Skull Base ◽  
Imaging Technique ◽  
Stimulated Raman Scattering ◽  
Tumor Resection ◽  
Chemical Information ◽  
Label Free ◽  
Skull Base Tumors ◽  
Fresh Tissue ◽  
Stimulated Raman

AbstractIntraoperative consultations, used to guide tumor resection, can present histopathological findings that are challenging to interpret due to artefacts from tissue cryosectioning and conventional staining. Stimulated Raman histology (SRH), a label-free imaging technique for unprocessed biospecimens, has demonstrated promise in a limited subset of tumors. Here, we target unexplored skull base tumors using a fast simultaneous two-channel stimulated Raman scattering (SRS) imaging technique and a new pseudo-hematoxylin and eosin (H&E) recoloring methodology. To quantitatively evaluate the efficacy of our approach, we use modularized assessment of diagnostic accuracy beyond cancer/non-cancer determination and neuropathologist confidence for SRH images contrasted to H&E-stained frozen and formalin-fixed paraffin-embedded (FFPE) tissue sections. Our results reveal that SRH is effective for establishing a diagnosis using fresh tissue in most cases with 87% accuracy relative to H&E-stained FFPE sections. Further analysis of discrepant case interpretation suggests that pseudo-H&E recoloring underutilizes the rich chemical information offered by SRS imaging, and an improved diagnosis can be achieved if full SRS information is used. In summary, our findings show that pseudo-H&E recolored SRS images in combination with lipid and protein chemical information can maximize the use of SRS during intraoperative pathologic consultation with implications for tissue preservation and augmented diagnostic utility.

scholarly journals A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

The Analyst ◽  
2018 ◽  
Vol 143 (20) ◽  
pp. 4844-4848 ◽  
Author(s):  
Chen Zeng ◽  
Fanghao Hu ◽  
Rong Long ◽  
Wei Min
Keyword(s):  
Hydrogen Sulfide ◽  
Raman Scattering ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Stimulated Raman Scattering ◽  
Living Cells ◽  
Live Cell ◽  
Raman Probe ◽  
Stimulated Raman

We develop a ratiometric Raman probe for visualizing hydrogen sulfide in living cells as the first alkyne-based sensor for SRS microscopy.

scholarly journals Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy

2017 ◽  
Vol 8 (8) ◽  
pp. 5606-5615 ◽  
Author(s):  
William J. Tipping ◽  
Martin Lee ◽  
Alan Serrels ◽  
Valerie G. Brunton ◽  
Alison N. Hulme
Keyword(s):  
Raman Scattering ◽  
Therapeutic Response ◽  
Stimulated Raman Scattering ◽  
Living Cells ◽  
Drug Uptake ◽  
Drug Concentrations ◽  
Stimulated Raman

Stimulated Raman scattering (SRS) microscopy in tandem with bioorthogonal Raman labelling enables intracellular drug concentrations, distribution and therapeutic response to be measured in living cells.

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