scholarly journals Rapid and ultrasensitive detection of endocrine disrupting chemicals using a nanosensor-enabled cell-based platform

2017 ◽  
Vol 53 (62) ◽  
pp. 8794-8797 ◽  
Author(s):  
Ngoc D. B. Le ◽  
Xian Wang ◽  
Yingying Geng ◽  
Rui Tang ◽  
Gulen Yesilbag Tonga ◽  
...  
Keyword(s):  
High Throughput ◽  
Endocrine Disrupting Chemicals ◽  
Ultrasensitive Detection ◽  
Highly Sensitive ◽  
Endocrine Disrupting

A high-throughput cell-based nanosensor provides highly sensitive identification of estrogenic agents.

A high throughput screening method for endocrine disrupting chemicals in tap water and milk samples based on estrogen receptor α and gold nanoparticles

2020 ◽  
Vol 12 (2) ◽  
pp. 200-204 ◽  
Author(s):  
Mengyue Liu ◽  
Shengqiang Zhang ◽  
Shuyuan Du ◽  
Shuxue Pang ◽  
Xiaoyu Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Tap Water ◽  
Endocrine Disrupting Chemicals ◽  
Screening Method ◽  
Estrogen Receptor Α ◽  
Food Samples ◽  
Screening Methods ◽  
Estrogenic Effects ◽  
Endocrine Disrupting

Increasing concern over endocrine disrupting chemicals (EDCs) in environmental and food samples has created the demand for rapid and high throughput screening methods to evaluate their estrogenic effects.

scholarly journals Rapid, High-Throughput Detection of Endocrine Disrupting Chemicals Using Autobioluminescent Cellular Bioreporters

2018 ◽  
Author(s):  
Tingting Xu ◽  
Andrew Kirkpatrick ◽  
Jody Toperzer ◽  
Marvin Steven Furches ◽  
Steven Ripp ◽  
...  
Keyword(s):  
High Throughput ◽  
Endocrine Disrupting Chemicals ◽  
Endocrine Disrupting ◽  
High Throughput Detection

scholarly journals Single-Cell Distribution Analysis of AR Levels by High-Throughput Microscopy in Cell Models: Application for Testing Endocrine-Disrupting Chemicals

2020 ◽  
Vol 25 (7) ◽  
pp. 684-694
Author(s):  
Fabio Stossi ◽  
Ragini M. Mistry ◽  
Pankaj K. Singh ◽  
Hannah L. Johnson ◽  
Maureen G. Mancini ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Lines ◽  
High Throughput ◽  
Endocrine Disrupting Chemicals ◽  
Male Reproduction ◽  
Control Analysis ◽  
Distribution Analysis ◽  
Cell Distribution ◽  
Protein Levels ◽  
Endocrine Disrupting

Cell-to-cell variation of protein expression in genetically homogeneous populations is a common biological trait often neglected during analysis of high-throughput (HT) screens and is rarely used as a metric to characterize chemicals. We have captured single-cell distributions of androgen receptor (AR) nuclear levels after perturbations as a means to evaluate assay reproducibility and characterize a small subset of chemicals. AR, a member of the nuclear receptor family of transcription factors, is the central regulator of male reproduction and is involved in many pathophysiological processes. AR protein levels and nuclear localization often increase following ligand binding, with dihydrotestosterone (DHT) being the natural agonist. HT AR immunofluorescence imaging was used in multiple cell lines to define single-cell nuclear values extracted from thousands of cells per condition treated with DHT or DMSO (control). Analysis of numerous biological replicates led to a quality control metric that takes into account the distribution of single-cell data, and how it changes upon treatments. Dose–response experiments across several cell lines showed a large range of sensitivity to DHT, prompting us to treat selected cell lines with 45 Environmental Protection Agency (EPA)-provided chemicals that include many endocrine-disrupting chemicals (EDCs); data from six of the compounds were then integrated with orthogonal assays. Our comprehensive results indicate that quantitative single-cell distribution analysis of AR protein levels is a valid method to detect the potential androgenic and antiandrogenic actions of environmentally relevant chemicals in a sensitive and reproducible manner.

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