Near infrared two photon imaging using a bright cationic Yb(iii) bioprobe spontaneously internalized into live cells

2017 ◽  
Vol 53 (44) ◽  
pp. 6005-6008 ◽  
Author(s):  
Anh Thy Bui ◽  
Maryline Beyler ◽  
Alexei Grichine ◽  
Alain Duperray ◽  
Jean-Christophe Mulatier ◽  
...  
Keyword(s):  
Near Infrared ◽  
Macrocyclic Ligand ◽  
Live Cells ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

An Yb(iii) complex based on a dimethyl cyclen macrocyclic ligand is used as two-photon NIR bioprobe.

scholarly journals Amino-Functionalized Nitrogen-Doped Graphene-Quantum-Dot-Based Nanomaterials with Nitrogen and Amino-Functionalized Group Content Dependence for Highly Efficient Two-Photon Bioimaging

2020 ◽  
Vol 21 (8) ◽  
pp. 2939 ◽  
Author(s):  
Wen-Shuo Kuo ◽  
Chia-Yuan Chang ◽  
Keng-Shiang Huang ◽  
Jui-Chang Liu ◽  
Yu-Ting Shao ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Near Infrared ◽  
Graphene Quantum Dots ◽  
Lower Amount ◽  
Nitrogen Doped ◽  
Two Photon ◽  
Nitrogen Doped Graphene ◽  
Doped Graphene ◽  
Photon Imaging ◽  
Two Photon Imaging

We fabricated nanomaterials comprising amino-functionalized and nitrogen-doped graphene quantum dots (amino-N-GQDs) and investigated their photostability and intrinsic luminescence in the near-infrared spectrum to determine their suitability as contrast agents in two-photon imaging (TPI). We observed that amino-N-GQDs with a higher amount of bonded nitrogen and amino-functionalized groups (6.2%) exhibited superior two-photon properties to those with a lower amount of such nitrogen and groups (4.9%). These materials were conjugated with polymers containing sulfur (polystyrene sulfonate, PSS) and nitrogen atoms (polyethylenimine, PEI), forming amino-N-GQD–PSS–PEI specimens (amino-N-GQD-polymers). The polymers exhibited a high quantum yield, remarkable stability, and notable two-photon properties and generated no reactive oxygen species, rendering them excellent two-photon contrast agents for bioimaging. An antiepidermal growth factor receptor (AbEGFR) was used for labeling to increase specificity. Two-photon imaging (TPI) of amino-N-GQD (6.2%)-polymer-AbEGFR-treated A431 cancer cells revealed remarkable brightness, intensity, and signal-to-noise ratios for each observation at a two-photon excitation power of 16.9 nJ pixel−1 under 30 scans and a three-dimensional (3D) depth of 105 µm, indicating that amino-N-GQD (6.2%)-polymer-AbEGFR-treated cells can achieve two-photon luminescence with 71 times less power required for two-photon autofluorescence (1322.8 nJ pixel−1 with 500 scans) of similar intensity. This economy can minimize photodamage to cells, rendering amino-N-GQD-polymers suitable for noninvasive 3D bioimaging.

Multicolor, One- and Two-Photon Imaging of Enzymatic Activities in Live Cells with Fluorescently Quenched Activity-Based Probes (qABPs)

2011 ◽  
Vol 133 (31) ◽  
pp. 12009-12020 ◽  
Author(s):  
Mingyu Hu ◽  
Lin Li ◽  
Hao Wu ◽  
Ying Su ◽  
Peng-Yu Yang ◽  
...  
Keyword(s):  
Enzymatic Activities ◽  
Live Cells ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

Two-photon imaging of formaldehyde in live cells and animals utilizing a lysosome-targetable and acidic pH-activatable fluorescent probe

2017 ◽  
Vol 53 (48) ◽  
pp. 6520-6523 ◽  
Author(s):  
Xilei Xie ◽  
Fuyan Tang ◽  
Xiaoyan Shangguan ◽  
Shiyi Che ◽  
Jinye Niu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Systems ◽  
Live Cells ◽  
Acidic Ph ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

A two-photon fluorescent probe presented lysosomal targetability and an acidic pH-activatable response toward formaldehyde, and was applied to visualize formaldehyde in living systems.

Novel nonplanar and rigid fluorophores with intensive emission in water and the application in two-photon imaging of live cells

RSC Advances ◽  
2016 ◽  
Vol 6 (75) ◽  
pp. 71624-71627 ◽  
Author(s):  
Jingwen Zhang ◽  
Chao Wang ◽  
Lei Zhang ◽  
Huijing Wu ◽  
Yi Xiao ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Extinction Coefficient ◽  
Molar Extinction Coefficient ◽  
Live Cells ◽  
Two Photon ◽  
Yield Value ◽  
Photon Imaging ◽  
Two Photon Imaging

Novel nonplanar and rigid fluorophores have been synthesized, by fusing a twisted heterocycle onto a naphthalimide skeleton, and exhibited excellent higher quantum yield value (Φ = 0.60–0.66) and molar extinction coefficient in water.

scholarly journals Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e51980 ◽  
Author(s):  
Kaspar Podgorski ◽  
Ewald Terpetschnig ◽  
Oleksii P. Klochko ◽  
Olena M. Obukhova ◽  
Kurt Haas
Keyword(s):  
Near Infrared ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

In Vivo Near-Infrared Two-Photon Imaging of Amyloid Plaques in Deep Brain of Alzheimer’s Disease Mouse Model

2018 ◽  
Vol 9 (12) ◽  
pp. 3128-3136 ◽  
Author(s):  
Congping Chen ◽  
Zhuoyi Liang ◽  
Biao Zhou ◽  
Xuesong Li ◽  
Caleb Lui ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
Near Infrared ◽  
Amyloid Plaques ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Deep Brain
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